Evidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro

Gianfranceschi, G.; Amici, Domenico; Guglielmi, L.

doi:10.1016/0005-2787(75)90121-5

Cited by 38 publications

(7 citation statements)

References 19 publications

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“…Co.) as in ref. 11 (1,2). Uptake of labeled uridine after 1-to 2-min pulses was comparable in the presence and in the absence of the factor, the addition of which, therefore, did not impair uptake of the label.…”

Section: Methodsmentioning

confidence: 67%

“…Its biological activity cannot be attributed to the presence of a nuclease or of a histone fragment. Control of chromatin and DNA transcription is apparently mediated by an ionic interaction of the peptide with the deoxyribosephosphate backbone of DNA (3) and takes place at the level of the initiation reaction (1,2). Accordingly, the factor does not inhibit the transcription of endogenous residual RNA polymerase bound to chromatin preparations (2).…”

mentioning

confidence: 99%

“…This factor causes a strong inhibition of DNA-directed (1) or chromatin-directed (2) RNA polymerase reactions in vitro. Its biological activity cannot be attributed to the presence of a nuclease or of a histone fragment.…”

mentioning

confidence: 99%

“…We have previously isolated, from aqueous extracts of calf thymus, an active factor, which has been partially purified by DEAE-cellulose and Dowex 50WX2 chromatography and characterized as a peptide of less than 5000 molecular weight (1). This factor causes a strong inhibition of DNA-directed (1) or chromatin-directed (2) RNA polymerase reactions in vitro.…”

mentioning

confidence: 99%

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Low-molecular-weight peptide inhibits RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells.

Amici¹,

Rossi²,

Cioé³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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The RNA synthesis of human leukemic leukocytes and phytohemagglutinin-stimulated lymphocytes is markedly reduced by administration of a low-molecular-weight nonhistone peptide factor from calf thymus. Treatment with the factor strongly inhibits hemoglobin production and globin mRNA transcription in dimethyl sulfoxide-stimulated Friend cells without appreciably modifying the rate of cell growth. Evidence for specificity of these effects is provided by the lack of action of the factor on both growth rate and RNA synthesis of a number of nondifferentiating cell lines from various animal species. After removal of the compound, both human lymphocytes and Friend cells can be stimjK'a&ed by phytohemagglutinin and by dimethyl sulfoxide, respectively, ruling out any toxic effect.We have previously isolated, from aqueous extracts of calf thymus, an active factor, which has been partially purified by DEAE-cellulose and Dowex 50WX2 chromatography and characterized as a peptide of less than 5000 molecular weight (1). This factor causes a strong inhibition of DNA-directed (1) or chromatin-directed (2) RNA polymerase reactions in vitro.

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Section: Methodsmentioning

confidence: 67%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Low-molecular-weight peptide inhibits RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells.

Amici¹,

Rossi²,

Cioé³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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“…Other liver age-related modifications, such as the increased volume and number of tetraploid nuclei, can be corrected by grafting a neonatal thymus into old mice (Pieri et al 1980;Pieri et al 1982). Results similar to those obtained by grafting a neonatal thymus have been achieved using mice treated with the calf thymus extract (TME) (Rossolini et al 1992;Basso et al 1993) Old rats treated with TME, prepared and characterized on the basis of non-immunological test, are capable of recovering altered glycogen and lipid levels, RNA/DNA ratio and transcriptional activity of RNA polymerase DNA-dependent in the liver (Gianfranceschi et al 1974;Gianfranceschi et al 1975;Amici et al 1983).…”

Section: Introductionmentioning

confidence: 92%

Aging Reversibility: from Thymus Graft to Vegetable Extract Treatment – Application to Cure an Age-associated Pathology

et al. 2005

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Neonatal thymus graft and thymus calf extract (TME) in vivo treatment exert similar corrective actions on different mouse age-related alterations. The aim of the present paper is to investigate whether a vegetal extract, wheat sprout extract (WESPRE), could mimic the thymus action on recovering age-related alterations and if this extract can cure an age-associated pathology, the cataract in dogs. Present experiments were carried out by using WESPRE and TME in vivo in old mice to check their ability to recover the altered DNA synthesis in hepatocyte primary cultures. Old mice treated with WESPRE and TME showed a recovery of hepatocyte DNA synthesis levels when compared with the old untreated ones. The increase of DNA and protein contents observed in aged animals is reduced by WESPRE treatments to levels observed in young mice hepatocytes. We measured also WESPRE phosphorylation activity by endogenous kinase: it was from 10 to 40 times higher with respect to wheat seeds. Old dogs were orally treated for a month and the lens opacity analysed before and after the treatment. Results showed a reduction from 25 to 40% of lens opacity. The efficacy of wheat sprouts in the recovery of age-related alterations and in treating age-associated pathologies could be due to the contemporary presence of small regulatory acid peptides, a remarkable level of highly energetic phosphoric radicals and antioxidant molecules, peculiarities that may be, to some extent, related to the aging process regulation.

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Proteins from the Prokaryotic Nucleoid: Biochemical and 1H NMR Studies on Three Bacterial Histone-Like Proteins

Lammi

Paci

Pon

et al. 1984

Advances in Experimental Medicine and Biology

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Evidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro

Cited by 38 publications

References 19 publications

Low-molecular-weight peptide inhibits RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells.

Low-molecular-weight peptide inhibits RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells.

Aging Reversibility: from Thymus Graft to Vegetable Extract Treatment – Application to Cure an Age-associated Pathology

Proteins from the Prokaryotic Nucleoid: Biochemical and 1H NMR Studies on Three Bacterial Histone-Like Proteins

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