Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

Nascimento, Francisco X.; Vicente, Cláudia S. L.; Barbosa, Pedro; Espada, Margarida; Glick, Bernard R.; Mota, Manuel; Oliveira, Solange

doi:10.1007/s10526-012-9500-0

Cited by 55 publications

(37 citation statements)

References 40 publications

(48 reference statements)

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“…The PWN was reproduced in Erlenmeyer flasks as described by Nascimento et al . (). After extraction, nematodes were washed with 3% hydrogen peroxide for 10 min (Han et al ., ) followed by washes in sterilized 0.03 M MgSO 4 to remove the hydrogen peroxide residue.…”

Section: Methodsmentioning

confidence: 99%

Non‐specific transient mutualism between the plant parasitic nematode, Bursaphelenchus xylophilus, and the opportunistic bacterium Serratia quinivorans BXF1, a plant‐growth promoting pine endophyte with antagonistic effects

Nascimento

Espada

Barbosa

et al. 2016

Environmental Microbiology

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The aim of this study is to understand the biological role of Serratia quinivorans BXF1, a bacterium commonly found associated with Bursaphelenchus xylophilus, the plant parasitic nematode responsible for pine wilt disease. Therefore, we studied strain BXF1 effect in pine wilt disease. We found that strain BXF1 promoted in vitro nematode reproduction. Moreover, the presence of bacteria led to the absence of nematode chitinase gene (Bxcht-1) expression, suggesting an effect for bacterial chitinase in nematode reproduction. Nevertheless, strain BXF1 was unable to colonize the nematode interior, bind to its cuticle with high affinity or protect the nematode from xenobiotic stress. Interestingly, strain BXF1 was able to promote tomato and pine plant-growth, as well as to colonize its interior, thus, acting like a plant-growth promoting endophyte. Consequently, strain BXF1 failed to induce wilting symptoms when inoculated in pine shoot artificial incisions. This bacterium also presented strong antagonistic activities against fungi and bacteria isolated from Pinus pinaster. Our results suggest that B. xylophilus does not possess a strict symbiotic community capable of inducing pine wilt disease symptoms as previously hypothesized. We show that bacteria like BXF1, which possess plant-growth promoting and antagonistic effects, may be opportunistically associated with B. xylophilus, possibly acquired from the bacterial endophytic community of the host pine.

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Section: Methodsmentioning

confidence: 99%

Non‐specific transient mutualism between the plant parasitic nematode, Bursaphelenchus xylophilus, and the opportunistic bacterium Serratia quinivorans BXF1, a plant‐growth promoting pine endophyte with antagonistic effects

Nascimento

Espada

Barbosa

et al. 2016

Environmental Microbiology

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“…Using ACC deaminase-producing bacteria in association with plants subjected to different kinds of biotic and abiotic stresses resulted in enhanced plant tolerance [18]–[25]. The use of ACC deaminase-producing bacteria in association with plants for purposes of soil decontamination is also documented [26]–[28].…”

Section: Introductionmentioning

confidence: 99%

New Insights into 1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase Phylogeny, Evolution and Ecological Significance

et al. 2014

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The main objective of this work is the study of the phylogeny, evolution and ecological importance of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, the activity of which represents one of the most important and studied mechanisms used by plant growth–promoting microorganisms. The ACC deaminase gene and its regulatory elements presence in completely sequenced organisms was verified by multiple searches in diverse databases, and based on the data obtained a comprehensive analysis was conducted. Strain habitat, origin and ACC deaminase activity were taken into account when analyzing the results. In order to unveil ACC deaminase origin, evolution and relationships with other closely related pyridoxal phosphate (PLP) dependent enzymes a phylogenetic analysis was also performed. The data obtained show that ACC deaminase is mostly prevalent in some Bacteria, Fungi and members of Stramenopiles. Contrary to previous reports, we show that ACC deaminase genes are predominantly vertically inherited in various bacterial and fungal classes. Still, results suggest a considerable degree of horizontal gene transfer events, including interkingdom transfer events. A model for ACC deaminase origin and evolution is also proposed. This study also confirms the previous reports suggesting that the Lrp-like regulatory protein AcdR is a common mechanism regulating ACC deaminase expression in Proteobacteria, however, we also show that other regulatory mechanisms may be present in some Proteobacteria and other bacterial phyla. In this study we provide a more complete view of the role for ACC deaminase than was previously available. The results show that ACC deaminase may not only be related to plant growth promotion abilities, but may also play multiple roles in microorganism's developmental processes. Hence, exploring the origin and functioning of this enzyme may be the key in a variety of important agricultural and biotechnological applications.

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“…This was consistent with the previous findings of Nascimento et al . () about the roles of P. putida UW4 in promoting pine tree seedling growth and reduction of PWD symptoms caused by the PWN. In the latter study, mechanisms of biocontrol were attributed to reduction of deleterious ethylene levels by 1‐aminocyclopropane‐1‐carboxylate (ACC) deaminase, produced by P. putida , and the direct plant growth promotion that potentially boosted plant defence.…”

Section: Discussionmentioning

confidence: 99%

Induction of resistance against pine wilt disease caused by Bursaphelenchus xylophilus using selected pine endophytic bacteria

et al. 2018

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Pine wilt disease (PWD) is the most destructive disease threatening pine worldwide. The disease is mainly caused by the pine wood nematode, Bursaphelenchus xylophilus, which is vectored by pine sawyer longhorn‐beetles, Monochamus spp. This study aimed to select resistance‐inducing pine endophytic bacteria for management of PWD. To set up a defence‐related genes expression pattern for screening, four chemical inducers (salicylic acid, γ‐aminobutyric acid (GABA), β‐aminobutyric acid and α‐aminobutyric acid) were tested in vitro on pine calli and in vivo on pine seedlings. Treatment with GABA had the greatest reduction in PWD severity on pine seedlings. The pattern of defence‐related gene expression in calli treated with GABA was used to select potential resistance‐inducing bacterial strains. In addition, 92 bacterial strains were isolated from pine tree needles and stems and were tested for expression of defence‐related genes in pine calli in vitro. Among the tested strains, 13 showed a similar pattern to GABA treatment in at least four tested defence‐related genes and were selected for the seedling assay. From the seedling assay, three bacterial strains (16YSM‐E48, 16YSM‐P180 and 16YSM‐P39) showed significant reduction in PWD severity compared to the untreated control. Moreover, among the selected strains, cell‐free culture supernatant of strain 16YSM‐P180 significantly reduced PWD severity in inoculated pine seedlings. The selected strains were identified based on the 16S rRNA sequence as Pseudomonas putida 16YSM‐E48, Curtobacterium pusillum 16YSM‐P180 and Stenotrophomonas rhizophila 16YSM‐P39. These selected strains are suggested as potential alternatives for management of PWD by induction of systemic resistance.

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Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

Cited by 55 publications

References 40 publications

Non‐specific transient mutualism between the plant parasitic nematode, Bursaphelenchus xylophilus, and the opportunistic bacterium Serratia quinivorans BXF1, a plant‐growth promoting pine endophyte with antagonistic effects

Non‐specific transient mutualism between the plant parasitic nematode, Bursaphelenchus xylophilus, and the opportunistic bacterium Serratia quinivorans BXF1, a plant‐growth promoting pine endophyte with antagonistic effects

New Insights into 1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase Phylogeny, Evolution and Ecological Significance

Induction of resistance against pine wilt disease caused by Bursaphelenchus xylophilus using selected pine endophytic bacteria

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