Evidence for the Expression of Alcohol Dehydrogenase Class I Gene in Rat Uterus and Its Up-regulation by Progesterone

Ohno, Tomoko; Hiroi, Hisahiko; Momoeda, Mikio; Hosokawa, Yumi; Tsutsumi, Ryo; Koizumi, Minako; Nakazawa, Fumiko; Yano, Tetsu; Tsutsumi, Osamu; Taketani, Yuji

doi:10.1507/endocrj.k07-082

Cited by 6 publications

(4 citation statements)

References 57 publications

(57 reference statements)

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“…Ohno et al have revealed the expression of ADH class I gene in rat endometrial stromal cells 7. Several reports have shown that the expression of the ADH class I gene is regulated by sex hormones.…”

Section: Discussionmentioning

confidence: 99%

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The activity of class I, II, III, and IV alcohol dehyrogenase isoenzymes and aldehyde dehydrogenase in endometrial cancer

Orywal

Jelski

Zdrodowski

et al. 2010

Clinical Laboratory Analysis

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Section: Discussionmentioning

confidence: 99%

“…ADH and ALDH exist in multiple molecular forms that have been grouped into several classes 6. Recent data show that the uterus contains ADH class I, III, and IV isoenzymes 7. These enzymes also play a significant role in the metabolism of many biological substances.…”

Section: Introductionmentioning

confidence: 99%

The activity of class I, II, III, and IV alcohol dehyrogenase isoenzymes and aldehyde dehydrogenase in endometrial cancer

Orywal

Jelski

Zdrodowski

et al. 2010

Clinical Laboratory Analysis

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show abstract

“…The oxytocin receptor (Oxtr), receptor for the hormone and neurotransmitter oxytocin, has been found expressed in both myometrium and endometrium of uterus [43], [44]. ADH1 is specifically expressed in endometrial stromal cells, up-regulated by progesterone and may regulate retinoic acid synthesis [45].…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Profiling of Mouse Uterus at Pre-Implantation Stage under VEGF Repression

Lü

Qin

et al. 2013

PLoS ONE

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Uterus development during pre-implantation stage affects implantation process and embryo growth. Aberrant uterus development is associated with many human reproductive diseases. Among the factors regulating uterus development, vascular remodeling promoters are critical for uterus function and fertility. Vascular endothelial growth factor (VEGF), as one of the major members, has been found to be important in endothelial cell growth and blood vessel development, as well as in non-endothelial cells. VEGF mediation in reproduction has been broadly studied, but VEGF-induced transcriptional machinery during implantation window has not been systematically studied. In this study, a genetically repressed VEGF mouse model was used to analyze uterus transcriptome at gestation 2.5 (G2.5) by Solexa/Illumina’s digital gene expression (DGE) system. A number of 831 uterus-specific and 2398 VEGF-regulated genes were identified. Gene ontology (GO) analysis indicated that genes actively involved in uterus development were members of collagen biosynthesis, cell proliferation and cell apoptosis. Uterus-specific genes were enriched in activities of phosphatidyl inositol phosphate kinase, histone H3-K36 demethylation and protein acetylation. Among VEGF-regulated genes, up-regulated were associated with RNA polymerase III activity while down-regulated were strongly related with muscle development. Comparable numbers of antisense transcripts were identified. Expression levels of the antisense transcripts were found tightly correlated with their sense expression levels, an indication of possibly non-specific transcripts generated around the active promoters and enhancers. The antisense transcripts with exceptionally high or low expression levels and the antisense transcripts under VEGF regulation were also identified. These transcripts may be important candidates in regulation of uterus development. This study provides a global survey on genes and antisense transcripts regulated by VEGF in the pre-implantation stage. Results will contribute to further study the candidate genes and pathways in regulating implantation process and related diseases.

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“…To isolate the genes regulated by P 4 in the rat uterus, we previously performed suppressive subtractive hybridization (Ohno et al 2008), and 14-3-3t was identified as one of the genes that were upregulated in the rat uterus after P 4 treatment. The 14-3-3 proteins form a family of highly conserved molecules that are expressed in a wide range of organisms and tissues (Obsilová et al 2008).…”

Section: Introductionmentioning

confidence: 99%

The progesterone-responsive gene 14-3-3τ enhances the transcriptional activity of progesterone receptor in uterine cells

Ito

Urano²,

Hiroi³

et al. 2012

Journal of Molecular Endocrinology

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Members of the 14-3-3 family are intracellular dimeric phosphoserine-binding proteins that can associate with and modulate the activities of many proteins. In our efforts to isolate the genes regulated by progesterone (P 4 ) using suppressive subtractive hybridization, we previously found that 14-3-3t is one of the genes upregulated by P 4 . In this study, we demonstrated by quantitative RT-PCR (qRT-PCR), western blot analyses, and immunohistochemistry that 14-3-3t mRNA and protein levels were increased in the rat uterus after P 4 treatment. Furthermore, qRT-PCR indicated that P 4 increased 14-3-3t mRNA levels in human endometrial epithelial cells and endometrial stromal cells (ESCs). Western blot and qRT-PCR analyses revealed that in vitro decidualization using cAMP and medroxyprogesterone 17-acetate increased levels of 14-3-3t mRNA and protein in ESCs. We have shown by qRT-PCR and western blot analyses that P 4 increased the mRNA and protein levels of 14-3-3t in Ishikawa cells that stably express P 4 receptor-B (PR-B). Immunocytochemistry revealed that 14-3-3t colocalizes with PR and translocates from the cytoplasm to the nucleus in response to P 4 . Moreover, by luciferase reporter assay, we demonstrated that 14-3-3t enhances the transcriptional activity of PR-B. Taken together, we propose that 14-3-3t is a P 4 -responsive gene in uterine cells that modulates P 4 signaling.

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Evidence for the Expression of Alcohol Dehydrogenase Class I Gene in Rat Uterus and Its Up-regulation by Progesterone

Cited by 6 publications

References 57 publications

The activity of class I, II, III, and IV alcohol dehyrogenase isoenzymes and aldehyde dehydrogenase in endometrial cancer

The activity of class I, II, III, and IV alcohol dehyrogenase isoenzymes and aldehyde dehydrogenase in endometrial cancer

Transcriptional Profiling of Mouse Uterus at Pre-Implantation Stage under VEGF Repression

The progesterone-responsive gene 14-3-3τ enhances the transcriptional activity of progesterone receptor in uterine cells

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