Evidence for the Existence of Puumula-Related Virus among Clethrionomys rufocanus in Hokkaido, Japan

Kariwa, Hiroaki; Yoshizumi, Shima; Arikawa, Jiro; Yoshimatsu, Kumiko; Takahashi, Ken­ichi; Takashima, Ikuo; Hashimoto, Nobuo

doi:10.4269/ajtmh.1995.53.222

Cited by 60 publications

(35 citation statements)

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“…The SPs were captured by anti-TBEV E antibodies coated on 96-well plates, and rodent serum that bound to the SPs was detected by alkaline phosphatase-conjugated anti-mouse IgG antibodies. The positive sera by ELISA were confirmed by neutralization test using the Oshima 5-10 strain of TBEV, which was isolated in Hokkaido in 1995, as described previously [7].…”

Section: )mentioning

confidence: 99%

Epizootiological Study of Tick-Borne Encephalitis Virus Infection in Japan

Yoshii

Mottate

Omori-Urabe

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. Rodent species that are potential hosts for TBEV are widely distributed in various regions in Japan. In this study, we carried out large-scale epizootiological surveys in rodents from various areas of Japan. A total of 931 rodent and insectivore sera were collected from field surveys. Rodents seropositive for TBEV were found in Shimane Prefecture in Honshu and in several areas of Hokkaido Prefecture. These results emphasize the need for further epizootiological and epidemiological research of TBEV and preventive measures for emerging tick-borne encephalitis in Japan.

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Section: )mentioning

confidence: 99%

Epizootiological Study of Tick-Borne Encephalitis Virus Infection in Japan

Yoshii

Mottate

Omori-Urabe

et al. 2011

The Journal of Veterinary Medical Science

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“…In addition, infected Rattus norvegicus and C. rufocanus have been found in Hokkaido [12,13]. Further, surveys in Sakhaline, Russia, also revealed the presence of a PUU-like virus in C. rufocanus (Unpublished data).…”

Section: Discussionmentioning

confidence: 92%

“…Although C. glareolus is considered the primary host species for PUU virus, the agent has also been isolated from C. rufocanus [29]. Recent studies in Hokkaido, Japan revealed the presence of PUUlike viruses in C. rufocanus [12,13]. Although human infection has not been reported in Japan, antibodies in human sera have been found in the general public and in high-risk groups that have close contacts with rodents [3].…”

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confidence: 99%

Development of an Efficient Method for Recovery of Puumala and Puumala-Related Viruses by Inoculation of Mongolian Gerbils

Lokugamage

Kariwa

Lokugamage

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Puumala (PUU) virus and PUU-related viruses are difficult to isolate in cell culture. To determine whether animal inoculation would be a better alternative for virus recovery, the Sotkamo strain of PUU virus was inoculated into several animal species. Newborn Mongolian gerbils (MGs), mice, and rats were infected with the Sotkamo strain by intracerebral (ic), intraperitoneal (ip), and subcutaneous (sc) inoculation. Antibodies to PUU appeared in MGs at 30 days post-infection (dpi), and in mice and rats at 15 dpi. Interestingly, virus appeared at 7 dpi in lung and brain of MGs inoculated via ic and ip routes. Virus was detected in all tested tissues of MGs at 15 dpi, with a peak level of 1.36 × 10 5 focus forming units (FFU)/g in brain tissue. The virus titer declined with the onset of the antibody response and became undetectable by 75 dpi, when the antibody titer reached the maximum level. The appearance of the virus in mice and rats was delayed as compared to MGs, and the virus titer was apparently lower, at approximately 4 to 8 × 10 3 FFU/g, at 15 dpi. In addition, lung homogenates of antibody-positive Clethrionomys (C.) rufocanus (captured in Tobetsu, Hokkaido, Japan) were inoculated into MGs by the ic route. PUU-related viral RNA was detected at 16 dpi in the brains of MG inoculated with the lung homogenate, and antibodies were detected at 45 dpi. These findings indicate that newborn MG inoculation is an efficient method to recover PUU and PUU-related viruses. KEY WORDS: Clethrionomys rufocanus, Hantavirus, Mongolian gerbil, Puumala virus, Rodent.J. Vet. Med. Sci. 65(11): 1189-1194, 2003 Hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in Europe and Asia, and hantavirus pulmonary syndrome (HPS) in the Americas [6,16,22,25,26]. It is believed that there is a close relationship between hantaviruses and the specific rodent species in which they establish inapparent and persistant infections [23]. Hantaan (HTN), Seoul (SEO), and Dobrava (DOB) viruses cause a severe form of HFRS and are carried by Murinae rodents [1,2,17,18]. Arvicolinae rodents are considered to be the reservoir hosts of Puumala (PUU), Tula (TUL), Topografov (TOP) and Khabarovsk (KBR) viruses [15,23]. Sigmodontine rodents are the most common rodent species in the Americas and are the reservoir animals for Sin Nombre (SN), Andes (AND), Bayou (BAY), Black Creek Canal (BCC), and New York (NY) viruses [7,10,11,19,20,24,27]. Primary hosts shed viruses into urine, saliva, and feces, and virus transmission to humans occurs via aerosolized rodent excreta [21,28] or via contaminated saliva in animal bites [8].Members of the genus Hantavirus of the family Bunyaviridae are negative strand RNA viruses with tripartite genomes. The segments have been designated, based on their sizes, as large (L), medium (M), and small (S). The L segment encodes a viral RNA polymerase, while M and S encode two surface glycoproteins G1 and G2, and a nucleocapsid protein (NP), respectively [9].PUU virus is the causative agent of a mild form of hemorrhagi...

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“…Therefore, studies on the transmission and maintenance of viruses in the rodent population are important for understanding the evolution of hantaviruses. Our repeated surveys in Kamiiso and Tobetsu revealed that SEOV and HOKV have been maintained stably for years in R. norvegicus and M. rufocanus, respectively (22,26). In both SEOV and HOKV, transmission appears to occur mainly by horizontal infection.…”

Section: Discussionmentioning

confidence: 96%

“…Although HOKV is widely distributed in Hokkaido (26,35), little is understood about the mode of infection of HOKV in M. rufocanus. Recently, Iwasa et al (20) reported that horizontal infection may be the main means of maintaining HOKV in the M. rufocanus population, as shown by microsatellite analysis.…”

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Mode of Infection of Hokkaido Virus (Genus Hantavirus) among Grey Red‐Backed Voles, Myodes rufocanus, in Hokkaido, Japan

Daud

Kariwa

Tanikawa

et al. 2007

Microbiology and Immunology

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Hokkaido virus (HOKV) is a member of the genus Hantavirus, in the family Bunyaviridae. To investigate HOKV infection in the host Myodes rufocanus, the grey red‐backed vole, 199 animals were captured at Tobetsu (October 2004 and July 2005) and Nakagawa (October 2004) in Hokkaido, Japan, for detection of antibody, antigen, and viral RNA. In the surveys in Tobetsu (2004) and Nakagawa (2004), seropositive animals were detected at a frequency of 6.0% (5/84) and 10.4% (5/48), respectively. No seropositive animals were detected in Tobetsu in 2005. Seroprevalence in males in Tobetsu and Nakagawa in 2004 was 25% (1/4) and 45.5% (5/11), respectively, which was higher than in females, at 5.0% (4/80) and 0% (0/37), respectively (P<0.01). These results suggest that male animals play an important role in the maintenance of HOKV in M. rufocanus. Two females were seronegative but viral RNA‐positive, indicating that these animals had acute infections before antibody was produced. Another five infected animals in Nakagawa were all male and had high levels of antibodies and viral RNA, suggesting that they had persistent infections. Viral RNA copies in organs of infected animals in Nakagawa were quantified by real‐time polymerase chain reaction. Two acutely infected animals had ≥10 times the number of RNA copies in their lungs compared to those of persistently infected animals. In most cases, lungs or spleen had the highest RNA copy number, regardless of infection status.

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Evidence for the Existence of Puumula-Related Virus among Clethrionomys rufocanus in Hokkaido, Japan

Cited by 60 publications

References 0 publications

Epizootiological Study of Tick-Borne Encephalitis Virus Infection in Japan

Epizootiological Study of Tick-Borne Encephalitis Virus Infection in Japan

Development of an Efficient Method for Recovery of Puumala and Puumala-Related Viruses by Inoculation of Mongolian Gerbils

Mode of Infection of Hokkaido Virus (Genus Hantavirus) among Grey Red‐Backed Voles, Myodes rufocanus, in Hokkaido, Japan

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