Evidence for the existence of a sulfonylurea-receptor-like protein in plants: Modulation of stomatal movements and guard cell potassium channels by sulfonylureas and potassium channel openers

Leonhardt, Nathalie; Marín, Elena; Vavasseur, Alain; Forestier, Cyrille Le

doi:10.1073/pnas.94.25.14156

Cited by 63 publications

(56 citation statements)

References 40 publications

(36 reference statements)

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“…In addition to their well-known role in detoxi®cation, pharmacological studies indicate that, some plant ABC transporters, like their animal counterparts, might catalyse or be involved in regulating ion¯uxes (Leonhardt et al, 1997(Leonhardt et al, , 1999. Using reverse genetics, we recently isolated a T-DNA insertion mutant for the Arabidopsis ABC-type transporter AtMRP5 (mrp5-1).…”

Section: Resultsmentioning

confidence: 99%

“…The AtMRP5 protein exhibits glutathion and glucuronide-conjugate transport activity when expressed in the yeast, Saccharomyces cerevisiae (Gaedeke et al, 2001). We have previously demonstrated that glibenclamide regulates plant ionic channels and induces stomatal opening in the dark in several plant species, including Vicia faba, Commelina communis (a monocotyledonous species) and Arabidopsis (Gaedeke et al, 2001;Leonhardt et al, 1997Leonhardt et al, , 1999Leonhardt et al, , 2001, providing strong evidence for a role of ABC transporters in guard cell signal transduction. When the AtMRP5 gene was disrupted by a T-DNA insertion, stomatal opening was no longer triggered by an application of glibenclamide (Gaedeke et al, 2001), indicating that AtMRP5 may function as a receptor for glibenclamide in guard cells, further strengthening the view that ABC transporters are instrumental for stomatal movements.…”

Section: Introductionmentioning

confidence: 98%

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The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signalling and water use

et al. 2003

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SummaryCarbon dioxide uptake and water release through stomata, controlling the opening and closure of stomatal pore size in the leaf surface, is critical for optimal plant performance. Stomatal movements are regulated by multiple signalling pathways involving guard cell ion channels. Using reverse genetics, we recently isolated a T-DNA insertion mutant for the Arabidopsis ABC-transporter AtMRP5 (mrp5-1). Guard cells from mrp5-1 mutant plants were found to be insensitive to the sulfonylurea compound glibenclamide, which in the wild type induces stomatal opening in the dark. Here, we report that the knockout in AtMRP5 affects several signalling pathways controlling stomatal movements. Stomatal apertures of mrp5-1 and wild-type Ws-2 were identical in the dark. In contrast, opening of stomata of mrp5-1 plants was reduced in the light. In the light, stomatal closure of mrp5-1 was insensitive to external calcium and abscisic acid, a phytohormone responsible for stomatal closure during drought stress. In contrast to Ws-2, the phytohormone auxin could not stimulate stomatal opening in the mutant in darkness. All stomatal phenotypes were complemented in transgenic mrp5-1 plants transformed with a cauli¯ower mosaic virus (CaMV) 35S-AtMRP5 construct. Both whole-plant and single-leaf gas exchange measurements demonstrated a reduced transpiration rate of mrp5-1 in the light. Excised leaves of mutant plants exhibited reduced water loss, and water uptake was strongly decreased at the whole-plant level. Finally, if plants were not watered, mrp5-1 plants survived much longer due to reduced water use. Analysis of CO 2 uptake and transpiration showed that mrp5-1 plants have increased water use ef®ciency. Mutant plants overexpressing AtMRP5 under the control of the CaMV 35S promoter again exhibited wild-type characteristics. These results demonstrate that multidrug resistance-associated proteins (MRPs) are important components of guard cell functioning.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signalling and water use

et al. 2003

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“…This suggestion was supported by pharmacological experiments that showed that sulfonylurea induces opening of stomata in a different species (6,19). To determine in which membrane we should focus our electrophysiological analyses we investigated the location of AtMRP5.…”

Section: Discussionmentioning

confidence: 96%

“…In addition, both of these membrane proteins are receptors for sulfonylureas (15,16) and are blocked by glibenclamide in numerous tissues (17,18). Leonhardt et al (6,19) showed that treatment with the sulfonylurea glibenclamide and other pharmacological compounds that interact with SURs and CFTR, modulate stomatal movements, and affect outward K ϩ and S-type anion channel properties. Subsequently, it was shown that glibenclamide-induced stomatal opening is impaired in Arabidopsis plants carrying a T-DNA insertion in the AtMRP5 gene, which encodes a multidrug resistance-associated protein.…”

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confidence: 99%

The ATP Binding Cassette Transporter AtMRP5 Modulates Anion and Calcium Channel Activities in Arabidopsis Guard Cells

Suh

Wang

Frelet

et al. 2007

Journal of Biological Chemistry

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106

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“…This stipulation is particularly interesting, given that highly K + -specific component mechanisms have been identified for both stomatal opening and closing, and large rates of ion flow must be established to facilitate prompt, and meaningful, stomatal response (see Fig. 2; Humble and Raschke 1971;Leonhardt et al 1997;Schroeder et al 1984Schroeder et al , 1987Schroeder 1988). Indeed, while some electrophysiological studies have shown that high Na + flux activity, in a domain similar to what is normally seen for K + , can be observed in guard cells (Zhao et al 2011;cf.…”

Section: Sodium As a Nutrientmentioning

confidence: 99%

Sodium as nutrient and toxicant

et al. 2013

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Background Sodium (Na + ) is one of the most intensely researched ions in plant biology and has attained a reputation for its toxic qualities. Following the principle of Theophrastus Bombastus von Hohenheim (Paracelsus), Na + is, however, beneficial to many species at lower levels of supply, and in some, such as certain C4 species, indeed essential. Scope Here, we review the ion's divergent roles as a nutrient and toxicant, focusing on growth responses, membrane transport, stomatal function, and paradigms of ion accumulation and sequestration. We examine connections between the nutritional and toxic roles throughout, and place special emphasis on the relationship of Na + to plant potassium (K + ) relations and homeostasis. Conclusions Our review investigates intriguing connections and disconnections between Na + nutrition and toxicity, and concludes that several leading paradigms in the field, such as on the roles of Na + influx and tissue accumulation or the cytosolic K + /Na + ratio in the development of toxicity, are currently insufficiently substantiated and require a new, critical approach.

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Evidence for the existence of a sulfonylurea-receptor-like protein in plants: Modulation of stomatal movements and guard cell potassium channels by sulfonylureas and potassium channel openers

Cited by 63 publications

References 40 publications

The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signalling and water use

The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signalling and water use

The ATP Binding Cassette Transporter AtMRP5 Modulates Anion and Calcium Channel Activities in Arabidopsis Guard Cells

Sodium as nutrient and toxicant

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