Evidence for the association of synaptotagmin with glutathione S-transferases: implications for a novel function in human breast cancer

Sreenath, A.; Kumar, K. Ravi; Reddy, Gorla V.; Sreedevi, B.; Praveen, D.; Mónika, Szilveszter; Selvaraj, S.; Reddy, M. Gopal; Reddanna, Pallu

doi:10.1016/j.clinbiochem.2005.01.009

Cited by 17 publications

(18 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is reasonable to speculate that a carcinogen metabolizing enzymes with lower activity could be associated with an elevated risk of developing cancer. However, to date, the po- tential association between genetic polymorphisms of GST-pi and breast carcinoma is still somewhat controversial and may vary from population to population [15][16][17][18][25][26][27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of the glutathione S-transferase P1 gene (GST-pi) in breast carcinoma

Khabaz¹

2014

pjp

View full text Add to dashboard Cite

Breast carcinoma is the most common cancer and cause of death among females worldwide, including Jordan. The risk factors for breast carcinoma are linked to DNA mutation and failure of DNA repair or detoxification systems. Identification of susceptibility factors that predispose individuals to breast carcinoma if they are exposed to particular environmental agents might give further insight into the etiology of this malignancy. The glutathione S-transferase (GST) enzyme family detoxifies carcinogenic compounds. Several genes that code for these enzymes are polymorphic, with particular genotypes previously shown to confer an increased carcinoma risk. The present study investigates GST-pi polymorphism in 100-tissue samples previously diagnosed as breast carcinoma, and in 48 non-cancer age-matched breast tissues, using the restriction fragment length polymorphism (RFLP) method for the polymerase chain reaction (PCR) product. Among breast cancer cases, 58%, 40% and 2% were homozygous (Ile/Ile), heterozygous (Ile/Val) and homozygous (Val/Val) respectively. In the control group, 58%, 37.5% and 4.2% were homozygous (Ile/Ile), heterozygous (Ile/Val), and homozygous (Val/Val) respectively. Our results did not support the involvement of GST-pi gene polymorphism in susceptibility to breast carcinoma in the tested North Jordanian female population.

show abstract

Section: Discussionmentioning

confidence: 99%

Polymorphism of the glutathione S-transferase P1 gene (GST-pi) in breast carcinoma

Khabaz¹

2014

pjp

View full text Add to dashboard Cite

show abstract

“…The differences in GSTA and GSTM expressions between normal and breast tumor tissue were not statistically significant (p> 0.05). Sreenath et al [19] found also a significant elevation in GSTP levels in breast cancer tissues with no appreciable changes in GSTA and GSTM compared to normal breast tissue using western blot analysis.…”

Section: Discussionmentioning

confidence: 99%

“…In most of the samples, the GST activities were higher in the tumor than those in the normal cytosolic fractions against both CDNB and EPNP. A number of studies showed that the GST activity toward several substrates including CDNB, DCNB, EAA, ENPP, MS in tumor tissue were significantly higher than those in normal breast tissue [7][8][9]19]. Kelley et al [20] reported that the average level of GSTs was substantially elevated in the cancer tissues than the levels in normal breast tissue from the same patient.…”

Section: Discussionmentioning

confidence: 99%

GST isoenzymes in matched normal and neoplastic breast tissue

Oğuztüzün

Abu-Hijleh²,

Çoban³

et al. 2011

neo

View full text Add to dashboard Cite

The potential to metabolize endogenous and exogenous substances may influence breast cancer development and tumor growth. Therefore we investigated GST activity and the protein expression of glutathione S-transferases (GSTs) isoenzymes known to be involved in the metabolism of endogenous and exogenous carcinogens in breast cancer tissue to obtain new information on their possible role in tumor progression.The interindividual variation in the conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) and of 1,2-epoxy-3-(p-nitrophenoxy) propane (EPNP) with glutathione (GSH) by cytosolic glutathione S-transferases (GSTs) were investigated in human breast matched normal and tumor samples. The GSTA, GSTM, GSTP and GSTT isoenzymes from the crude extracts of matched breast normal and tumor tissues in terms of their immunological properties using western blotting were compared.In most of the samples, the GST activities were higher in the tumor than in the normal cytosolic fractions against both CDNB and EPNP. In the western blotting analysis, it was proved statistically that in normal and tumor epithelial cells, there was difference between GST pi and theta isoenzymes expressions (p<0.05), but no difference between the staining scores of GST mu and alpha isoenzymes (p>0.05). In normal epithelium there was a stronger GST theta expression than in invasive tumor tissues (p=0.013). However, the stronger GST pi expression was observed in tumor epithelium than in normal epithelium in human breast cancers (p=0.000). We found the GSTP protein level and GST activities were higher in the breast tumor than in the normal cytosolic fractions against both CDNB and EPNP, thus implicating a certain biological importance.

show abstract

“…However, to date the results are still somewhat controversial and may vary from population to population. (Gilbert et al, 1993;Silvestrini et al, 1997;Buser et al, 1997;Helzlsouer et al, 1998;Millikan et al, 2000;Mitrunen et al, 2001;Gudmundsdottir et al, 2001;Huang et al, 2003;Egan et al, 2004;Sreenath et al, 2005;Moureau-Zabotto et al, 2006;Unlu et al, 2008;Saxena et al, 2009;Arun et al, 2010;Pongtheerat et al, 2011;Zhang et al, 2011;Ramalhinho et al, 2011;Aguiar et al, 2012;Ramalhinho et al, 2012;Saxena et al, 2013;Khabaz, 2014;Rodríguez et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies investigated the possible link between GSTP1 polymorphisms and breast cancer risk, and have shown conflicting results (Gilbert et al, 1993;Silvestrini et al, 1997;Buser et al, 1997;Helzlsouer et al, 1998;Millikan et al, 2000;Mitrunen et al, 2001;Gudmundsdottir et al, 2001;Huang et al, 2003;Egan et al, 2004;Sreenath et al, 2005;Moureau-Zabotto et al, 2006;Unlu et al, 2008;Saxena et al, 2009;Arun et al, 2010;Pongtheerat et al, 2011;Zhang et al, 2011;Ramalhinho et al, 2011;Aguiar et al, 2012;Ramalhinho et al, 2012;Khabaz, 2014;Rodríguez et al, 2014). Many previous studies revealed GSTP1 polymorphism has no correlation with clinical and pathologic tumor characteristics (Buser et al, 1997;Huang et al, 2003) only a few studies showed an inverse correlation with ER (Gilbert et al, 1993;Silvestrini et al, 1997;Moureau-Zabotto et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Association between GSTP1 Genotypes and Hormone Receptor Phenotype in Invasive Ductal Carcinomas of Breast

Khabaz

Gari²,

Al-Maghrabi³

et al. 2015

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Evidence for the association of synaptotagmin with glutathione S-transferases: implications for a novel function in human breast cancer

Cited by 17 publications

References 32 publications

Polymorphism of the glutathione S-transferase P1 gene (GST-pi) in breast carcinoma

Polymorphism of the glutathione S-transferase P1 gene (GST-pi) in breast carcinoma

GST isoenzymes in matched normal and neoplastic breast tissue

Association between GSTP1 Genotypes and Hormone Receptor Phenotype in Invasive Ductal Carcinomas of Breast

Contact Info

Product

Resources

About