Evidence for Ternary Complex Formation by Histone H1, DNA, and Liposomes

Kõiv, Anu; Palvimo, Jorma J.; Kinnunen, Paavo K.J.

doi:10.1021/bi00025a007

Cited by 51 publications

(45 citation statements)

References 62 publications

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“…Complexes composed exclusively of neutral (zwitterionic) lipids offer an alternative to CLs, in that they exhibit lower inherent cytotoxicity, 17,18 much longer circulation lifetimes, and very different clearance profiles. 19 Within this frame, some of the present authors have recently studied ternary complexes formed by the self-assembled association of neutral liposomes (L's), DNA, and bivalent metal cations in water solutions.…”

Section: Introductionmentioning

confidence: 99%

Structure and Phase Behavior of Self-Assembled DPPC−DNA−Metal Cation Complexes

et al. 2006

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Multilamellar liposomes of dipalmitoylphosphatidylcholine (DPPC) in solution with DNA and bivalent metal cations (Ca2+, Mn2+, Mg2+) self-assemble into a ternary DPPC-DNA-Me2+ complex. The supramolecular structure of the complex consists of an ordered multilamellar assembly where hydrated DNA helices are sandwiched between the lipid bilayers and the metal cations bind the phosphate groups of DNA to the lipid polar heads. In the range of explored incubation times, the complex coexists with the uncomplexed DPPC over the whole temperature range investigated (20-55 degrees C). Accordingly, two distinct coexisting lamellar phases are observed, one corresponding to the ternary complex and the other to the uncomplexed lipid. The structure and thermotropic phase behavior of both of these have been investigated by means of synchrotron X-ray diffraction, and the relevant structural data are deduced from experimental electron density profiles. While the uncomplexed lipid exhibits the same phase behavior as pure DPPC, that is, L beta'-P beta'-L alpha, the thermotropic behavior of the bound lipid in the complex is partially altered. This is manifested as an increase in the main transition temperature and the disappearance of the ripple phase leading to the single -phase transition. The role of the different metal cations in promoting and stabilizing the DNA condensation into the ternary complex is also discussed.

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Section: Introductionmentioning

confidence: 99%

Structure and Phase Behavior of Self-Assembled DPPC−DNA−Metal Cation Complexes

et al. 2006

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“…In animals, lipids in the PI pathway have been shown to interact with DNA and may regulate DNA replication (reviewed in Koiv et al, 1995; Kuvichkin, 2002). Studies have shown PtdInsP to decrease α, δ, and ε DNA polymerase activity (Shoji-Kawaguchi et al, 1995).…”

Section: The Pi Pathway and Dna Synthesismentioning

confidence: 99%

A Role for Phosphoinositides in Regulating Plant Nuclear Functions

Dieck¹,

Boss²,

Perera³

2012

Front. Plant Sci.

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Nuclear localized inositol phospholipids and inositol phosphates are important for regulating many essential processes in animal and yeast cells such as DNA replication, recombination, RNA processing, mRNA export and cell cycle progression. An overview of the current literature indicates the presence of a plant nuclear phosphoinositide (PI) pathway. Inositol phospholipids, inositol phosphates, and enzymes of the PI pathway have been identified in plant nuclei and are implicated in DNA replication, chromatin remodeling, stress responses and hormone signaling. In this review, the potential functions of the nuclear PI pathway in plants are discussed within the context of the animal and yeast literature. It is anticipated that future research will help shed light on the functional significance of the nuclear PI pathway in plants.

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“…In particular, the presence of histones and other positively charged proteins in chromatin raises the possibility that these proteins interact with negatively charged lipids in the nuclear membrane. In fact, several authors (10)(11)(12) have studied the interaction of histones with phosphatidylserine and other negatively charged lipids that exist in very minor amounts in the NE. In a previous study (13), we found that a variety of histone and histone combinations (H1, H2AH2B, H3H4, and octamers) caused a dose-dependent aggregation of phospholipid vesicles composed mainly of phosphatidylcholine (PC) to which 5-20 mol % phosphoinositides was added.…”

Section: Introductionmentioning

confidence: 99%

Histones Cause Aggregation and Fusion of Lipid Vesicles Containing Phosphatidylinositol-4-Phosphate

Lete

Sot

Gil

et al. 2015

Biophysical Journal

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In a previous article, we demonstrated that histones (H1 or histone octamers) interact with negatively charged bilayers and induce extensive aggregation of vesicles containing phosphatidylinositol-4-phosphate (PIP) and, to a lesser extent, vesicles containing phosphatidylinositol (PI). Here, we found that vesicles containing PIP, but not those containing PI, can undergo fusion induced by histones. Fusion was demonstrated through the observation of intervesicular mixing of total lipids and inner monolayer lipids, and by ultrastructural and confocal microscopy studies. Moreover, in both PI- and PIP-containing vesicles, histones caused permeabilization and release of vesicular aqueous contents, but the leakage mechanism was different (all-or-none for PI and graded release for PIP vesicles). These results indicate that histones could play a role in the remodeling of the nuclear envelope that takes place during the mitotic cycle.

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Evidence for Ternary Complex Formation by Histone H1, DNA, and Liposomes

Cited by 51 publications

References 62 publications

Structure and Phase Behavior of Self-Assembled DPPC−DNA−Metal Cation Complexes

Structure and Phase Behavior of Self-Assembled DPPC−DNA−Metal Cation Complexes

A Role for Phosphoinositides in Regulating Plant Nuclear Functions

Histones Cause Aggregation and Fusion of Lipid Vesicles Containing Phosphatidylinositol-4-Phosphate

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