Evidence for somatic translocation during potato dihaploid induction

Wilkinson, M. J.; Bennett, Simon T.; Clulow, Simon; Allainguillaume, Joël; Harding, Keith; Bennett, M. D.

doi:10.1038/hdy.1995.21

Cited by 52 publications

(40 citation statements)

References 29 publications

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“…Therefore, in present study, the chromosome translocation should occur in few somatic cells at low frequency. Somatic translocation has observed in dihaploid potato, this translocation had occurred during tissue culture (Wilkinson et al 1995). In Lolium multiflorum 9 Festuca arundinacea hybrids, somatic recombination were found in some unexpected diploid hybrids and this recombination was possibly caused by the combination treatments of colchicines, growth hormones and high sucrose concentration during embryo rescue (Pašakinskiene et al 1997).…”

Section: Discussionmentioning

confidence: 97%

Unequal chromosome division and inter-genomic translocation occurred in somatic cells of wheat–rye allopolyploid

Tang

Yan

et al. 2011

J Plant Res

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Newly synthesized wheat-rye allopolyploids were investigated by genomic in situ hybridization, over the first, second, third and fourth allopolyploid generations. Inter and intra chromosome connections were observed in 12 root-tip cells of CA4.4.7 (S(2) generation), and translocations between wheat and rye chromosomes were also detected in five root-tip cells. In root-tip cells of CA4.4.7.5 and CA4.4.7.2.2 (S(3) and S(4) generation), the chromosome connections occurred again, a dissociative small rye segment was detected in seven cells of CA4.4.7.5. In plants MSV6.1 and MSV6.5 (S(1) generation), almost half of the root-tip cells contained 13 rye chromosomes and the rest held 12 rye chromosomes, and all the cells of the two plants contained 42 wheat chromosomes. Five pairing configurations of rye chromosomes, including 5 II + 3 I, 6 II + 1 I, 6 II, 5 II + 2 I and 4 II + 4 I, were observed in pollen mother cells of the two plants. The two plants' progeny, including S(2), S(3), and S(4) generation plants, contained 42 wheat chromosomes and 12 rye chromosomes. Therefore, the inter chromosome translocation and unequal chromosome division could occur in somatic cells of wide hybrids. The unequal chromosome division in somatic cell could induce chromosome elimination at the early stages of allopolyploidization.

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Section: Discussionmentioning

confidence: 97%

Unequal chromosome division and inter-genomic translocation occurred in somatic cells of wheat–rye allopolyploid

Tang

Yan

et al. 2011

J Plant Res

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“…DNA introgression from the paternal parent cannot be explained by the hypothesis that a single sperm participated consequently in the single fertilization, indicating that the sperm cell from paternal parent may participate in fusion with the egg cell, and the chromosomes from paternal parent were eliminated in the subsequent divisions for some reason. Chromosome elimination and genetic introgression have been well documented in interspeciWc crossing of plants (Wilkinson et al 1995;Panakinskienñ et al 1997;Du et al 2008). There is some evidence of chromosome elimination in maternal haploid induction in maize.…”

Section: Discussionmentioning

confidence: 98%

Morphological and molecular evidences for DNA introgression in haploid induction via a high oil inducer CAUHOI in maize

Jin

et al. 2009

Planta

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The phenomenon of maternal haploid induction in maize was first described many years ago, but the underlying mechanism is still unclear. In this study, the Stock-6-derived, haploid-inducing line CAUHOI with high kernel oil content (KOC), was used as the pollinator to produce maternal haploids from the maize hybrid ZD958 with low KOC. CAUHOI is homozygous for the dominant marker gene R1-nj. Haploids were identified by morphological and cytological investigations. The frequency of haploid induction from this cross was 2.21%. Unexpectedly, many haploid kernels had weakly pigmented purple color on the embryo, and some haploid kernels had high KOC. Simple sequence repeat (SSR) analysis showed that 43.18% of the haploids carried segments from CAUHOI, and a small proportion (average 1.84%) of the genome of CAUHOI was introgressed into haploids. Haploid kernels with high KOC had a higher frequency of segment introgression from CAUHOI (2.92%) than that in haploid kernels with low KOC (1.79%), showing that the marker gene R1-nj and high-oil genes from CAUHOI were expressed during the development of some haploid embryos, and confirmed that the DNA introgression from the inducer parent occurred during maternal haploid induction. Together, these results suggested that the chromosome elimination was probably responsible for haploid induction in maize, and late somatic elimination might occur. Several possible mechanisms underlying haploid formation are discussed.

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“…Pasakinskiene et al (1997) reported that somatic recombination occurred in amphidiploids treated with colchicine during the diploidization process in Lolium multiflorum × Festuca arundinacea. Wilkinson et al (1995) observed that somatic translocation was not rare during dihaploid induction of potato. A part of the C genome unilaterally translocated to the other genomes without a loss of the C genome chromosomes in synthetic polyploid oats.…”

Section: Intergenomic Translocations Of C 1 Plantsmentioning

confidence: 99%

Production of Synthetic Polyploid Oats and Detection of C Genome Rearrangements by GISH and FISH

Ueno

Morikawa

2007

Breed. Sci.

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The genus Avena L. (Poaceae) comprises a polyploid series of diploid, tetraploid and hexaploid species with a basic chromosome number of seven. Four genomes (A, B, C and D) have been identified based on comparative karyotyping (Rajhathy and Thomas 1974). The genomic in situ hybridization (GISH) technique using A. eriantha's (CC) total DNA probe has been found to be very useful for distinguishing the C genome from the A/B/D genomes (Chen and Armstrong 1994). Hayasaki et al. (2000) reported that intergenomic translocations between the C and the other genomes were more frequent in hexaploid (AACCDD) than in tetraploid (AACC) species of oats. Fluorescence in situ hybridization (FISH) and RFLP of 18S-5.8S-26S rDNA enabled to clearly detect intraspecific chromosome variations in a wild tetraploid oat and rDNA was considered to be a suitable chromosome landmark (Hayasaki et al. 2001). reported that the presence and absence of intergenomic translocations between 7C and 17A/D chromosomes in hexaploid species determined A. sativa and A. byzantina, respectively. Therefore, it is possible to consider that the genomic constitution of polyploid oats had been changed through evolutionary polyploidization and hybridization. Especially, the C genome chromosomes of polyploid oats have probably tended to be transferred to the chromosomes of other genomes.Intergenomic translocations have been induced by chemical mutagens and radiation, and have played an important role in the genetics and breeding of several crop species (Lai and Srinivasachar 1979). In addition, colchicine has been extensively used for doubling the number of chromosomes of interspecific hybrids to obtain amphiploids. Consequently, we attempted to produce synthetic hexaploid and octoploid oats by doubling the number of chromosomes of interspecific hybrids, and to trace the behavior of the C genome chromosomes through artificial polyploidization.In the present paper, we reported the identification of C genome rearrangements in synthetic oats by GISH. FISH of 18S-5.8S-26S rDNA used as a probe was applied for detecting the satellite (SAT) chromosome carrying an intergenomic translocation segment. Evolutionary occurrence of intergenomic translocations in allopolyploid formation was also briefly discussed.Four combinations of interspecific crosses were made between A. strigosa (2n = 2x = 14; AsAs) or A. barbata (2n = 4x = 28; AABB) as a female parent and A. maroccana or A. murphyi (2n = 4x = 28; AACC) as a male parent. The reverse crosses were difficult to make due to the small size of the anthers and the occurrence of anthesis at midnight in the former species. The two F 1 hybrids using A. maroccana were abbreviated as F 1 str-mar and F 1 bar-mar, and the other two using A. murphyi were abbreviated as F 1 str-mur and F 1 bar-mur. These F 1 plants were treated with a 0.1% colchicine solution including 1% DMSO (dimethyl sulfoxide) and a few drops of Tween 20. The treatment was made by submerging the crown of the F 1 plants for 24 hrs to obtain chromosome doubling. After...

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Evidence for somatic translocation during potato dihaploid induction

Cited by 52 publications

References 29 publications

Unequal chromosome division and inter-genomic translocation occurred in somatic cells of wheat–rye allopolyploid

Unequal chromosome division and inter-genomic translocation occurred in somatic cells of wheat–rye allopolyploid

Morphological and molecular evidences for DNA introgression in haploid induction via a high oil inducer CAUHOI in maize

Production of Synthetic Polyploid Oats and Detection of C Genome Rearrangements by GISH and FISH

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