Evidence for serotonin synthesis-dependent regulation of in vitro neuronal firing rates in the midbrain raphe complex

Evans, Andrew K.; Reinders, Niels R.; Ashford, Katie A.; Christie, Isabel N.; Wakerley, J. B.; Lowry, Christopher A.

doi:10.1016/j.ejphar.2008.06.014

Cited by 38 publications

(35 citation statements)

References 74 publications

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“…At least 10 min between each treatment allowed for complete firing rate recovery, and the order of the SSRIs were counterbalanced. All solutions contained 3 μM phenylephrine hydrochloride and 40 μM L-tryptophan to maintain the spontaneous firing rate in the absence of noradrenergic tone and tryptophan availability (55). Data were analyzed for each drug by using a two-way RMANOVA.…”

Section: Methodsmentioning

confidence: 99%

Transgenic elimination of high-affinity antidepressant and cocaine sensitivity in the presynaptic serotonin transporter

Thompson¹,

Jessen²,

Henry³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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The authors note that, due to a printer's error, on page 3787, right column, first paragraph, lines 8-20, "Although significant evidence supports a primary role for the 5-HT transporter in the reinforcing properties of psychostimulants (35-37), SERT blockade also appears to contribute to reinforcement (38-40).Indeed, SERT appears to be primarily responsible for the sustained reinforcing properties of cocaine in the 5-HT transporter-KO mouse (22,23,(39)(40)(41). The significant compensatory alterations evident in 5-HT transporter-KO mice encouraged Chen and colleagues (42, 43) to develop a mouse bearing knock-in mutations in 5-HT transporter that, in vitro, reduced cocaine potency. Studies with these mice have yielded convincing evidence that 5-HT transporter is a key determinant of many synaptic and behavioral actions of cocaine" should instead appear as "Although significant evidence supports a primary role for the DA transporter in the reinforcing properties of psychostimulants (35-37), SERT blockade also appears to contribute to reinforcement (38-40). Indeed, SERT appears to be primarily responsible for the sustained reinforcing properties of cocaine in the DA transporter-KO mouse (22,23,(39)(40)(41). The significant compensatory alterations evident in DA transporter-KO mice encouraged Chen and colleagues (42,43) to develop a mouse bearing knock-in mutations in the DA transporter that, in vitro, reduced cocaine potency. Studies with these mice have yielded convincing evidence that the DA transporter is a key determinant of many synaptic and behavioral actions of cocaine." www.pnas.org/cgi

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Section: Methodsmentioning

confidence: 99%

Transgenic elimination of high-affinity antidepressant and cocaine sensitivity in the presynaptic serotonin transporter

Thompson¹,

Jessen²,

Henry³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…44 To investigate the effects of genotype and sex on 5-HT metabolism within these anxiety-related circuits, the midline DRN, dorsal part (DRD) and DRN, caudal part (DRC) and bilateral BNST, central amygdaloid (CeA), BLA, CA1 region of the ventral hippocampus (CA1v), lateral entorhinal cortex (LEnt) and subiculum (S) were selected for microdissection (see Figure 5, Supplementary Figure S3 and Table 1) and identified by comparisons with a standard mouse brain stereotaxic atlas. 45 Microdissected tissue punches in acetate buffer were thawed at 4 1C and centrifuged (13 000 r.p.m.…”

Section: Animalsmentioning

confidence: 99%

“…Chromatographic separation and electrochemical detection were accomplished using a previously described method. 44 …”

Section: Animalsmentioning

confidence: 99%

Urocortin-1 and -2 double-deficient mice show robust anxiolytic phenotype and modified serotonergic activity in anxiety circuits

et al. 2009

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The urocortin (Ucn) family of neuropeptides is suggested to be involved in homeostatic coping mechanisms of the central stress response through the activation of corticotropin-releasing factor receptor type 2 (CRFR2). The neuropeptides, Ucn1 and Ucn2, serve as endogenous ligands for the CRFR2, which is highly expressed by the dorsal raphe serotonergic neurons and is suggested to be involved in regulating major component of the central stress response. Here, we describe genetically modified mice in which both Ucn1 and Ucn2 are developmentally deleted. The double knockout mice showed a robust anxiolytic phenotype and altered hypothalamic-pituitary-adrenal axis activity compared with wild-type mice. The significant reduction in anxiety-like behavior observed in these mice was further enhanced after exposure to acute stress, and was correlated with the levels of serotonin and 5-hydroxyindoleacetic acid measured in brain regions associated with anxiety circuits. Thus, we propose that the Ucn/ CRFR2 serotonergic system has an important role in regulating homeostatic equilibrium under challenge conditions.

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“…5-HT 1A autoreceptors inhibit tonic firing activity of serotonergic neurons (Hajos et al, 2001;Evans et al, 2008) and hence decrease 5-HT release into the synaptic cleft (Parsons et al, 2001;Bundgaard et al, 2006). Additional studies reported inverse relationships between serotonin synthesis and heteroreceptor binding (Frey et al, 2008) and behavioral responses (Cowen et al, 1996;Heslop and Curzon, 1999).…”

Section: Association Of 5-ht 1a Bp Nd Between Autoreceptors and Hetermentioning

confidence: 99%

“…In the human raphe nuclei, the vast majority (80 -100%) of 5-HT 1A receptors are located on cell bodies of serotonin neurons (Hornung, 2010) and have an autoinhibitory function (Fink and Gothert, 2007). Thus, activation of 5-HT 1A autoreceptors decreases the rate of serotonergic cell firing (Hajos et al, 2001;Evans et al, 2008), leading to a reduced 5-HT release (Parsons et al, 2001;Bundgaard et al, 2006). In contrast, outside the raphe region 5-HT 1A receptors are mainly situated on non-5-HT-containing neurons in projection areas [i.e., as heteroreceptors (Mengod et al, 2010)], inhibiting postsynaptic cell firing (Fink and Gothert, 2007).…”

Section: Introductionmentioning

confidence: 99%

Escitalopram Enhances the Association of Serotonin-1A Autoreceptors to Heteroreceptors in Anxiety Disorders

Hahn¹,

Lanzenberger²,

Wadsak³

et al. 2010

J. Neurosci.

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Evidence for serotonin synthesis-dependent regulation of in vitro neuronal firing rates in the midbrain raphe complex

Cited by 38 publications

References 74 publications

Transgenic elimination of high-affinity antidepressant and cocaine sensitivity in the presynaptic serotonin transporter

Transgenic elimination of high-affinity antidepressant and cocaine sensitivity in the presynaptic serotonin transporter

Urocortin-1 and -2 double-deficient mice show robust anxiolytic phenotype and modified serotonergic activity in anxiety circuits

Escitalopram Enhances the Association of Serotonin-1A Autoreceptors to Heteroreceptors in Anxiety Disorders

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