Evidence for Rod‐Shaped DNA‐Stabilized Silver Nanocluster Emitters

Schultz, Danielle M.; Gardner, Kira; Oemrawsingh, S. S. R.; Markešević, Nemanja; Olsson, Kevin; Debord, Mark; Bouwmeester, Dirk; Gwinn, E. G.

doi:10.1002/adma.201204624

Cited by 181 publications

(435 citation statements)

References 57 publications

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“…Since it is more difficult to produce single crystals with DNA, mass spectrometry is a more widely used tool. In a recent report, Gwinn and co-workers screened 80 DNA sequences and used multiple stages of HPLC to purify products that are stable for the HPLC process [79]. With extensive mass spectrometry and simulation work, the authors concluded that DNA stabilized AgNCs contain roughly the same amounts of neutral silver atom and silver cation.…”

Section: Characterization Of Ncsmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

195

141

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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Section: Characterization Of Ncsmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

195

141

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“…The spectra showed the distinct Gaussian shape associated with AgNC emission. [ 14,25,27,31 ] Each spectrum was fi tted to a Gaussian function to determine the emission maximum. A histogram of the average emission maxima of all the single molecules is shown in Figure 2 d along with the emission spectrum of the bulk sample.…”

Section: Single Molecule Measurements: Emission Spectra Versus Decay mentioning

confidence: 99%

“…In this case, we focus on the nearinfrared emitters using 633 nm as the excitation wavelength. Although a number of single molecule studies have been performed on AgNC to investigate specifi c photophysical parameters, [ 5,6,8,10,14,25,[27][28][29][30][31][32][33] we simultaneously measure several photophysical properties for each individual cluster, including decay times, emission spectra, and antibunching. This allows us to compare and correlate these photophysical properties within a sample to better understand the photophysical behavior and heterogeneity of these complex as-synthesized AgNC samples.…”

Section: Introductionmentioning

confidence: 99%

Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

Hooley

Paolucci

Liao

et al. 2015

Advanced Optical Materials

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In this paper, single molecule spectroscopy is used to probe the photophysical heterogeneity of near‐infrared emitting silver nanoclusters stabilized in single stranded DNA containing 24 cytosines (C24‐AgNCs). Focusing on this subset of emitters allows us to address and correlate their photophysical parameters. The results suggest that for these near‐infrared emitting C24‐AgNCs, the broad range of observed photophysical properties is due to the spectral heterogeneity of two species, one of which has a broad range of emission maxima and decay times. This conclusion is drawn by analyzing the results of a large number of single C24‐AgNCs where, for the first time, emission maxima, fluorescence decay times, fluorescence intensity, blinking, and photon antibunching are determined simultaneously. The single molecule measurements also reveal that some of the C24‐AgNCs can change their spectral properties during the measurement, while photon antibunching measurements verified that all the analyzed C24‐AgNCs behave as single emitters. While the overall spectral heterogeneity can be related to the immobilization in the polymer film and/or intrinsic heterogeneity of the C24‐AgNCs emitters, the exact origin of the dynamical changes has not been fully determined. However, changes in the AgNCs geometry, local environment, or changes in the DNA conformation are possible explanations.

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“…In addition, different DNA sequences produce various emission colors [1][2][3][4][5][6], allowing multiplexed detection. The emission color is likely to relate to the arrangement of silver species and their interaction with the surrounding nucleotides [6].…”

Section: Introductionmentioning

confidence: 99%

Towards understanding of poly-guanine activated fluorescent silver nanoclusters

et al. 2014

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It has been recently reported that the fluorescence of some DNA templated silver nanoclusters (AgNCs) can be significantly enhanced upon hybridizing with a partially complementary DNA containing a Grich overhang near the AgNCs. This discovery has found a number of analytical applications but many fundamental questions remain to be answered. In this work, the photostability of these activated AgNCs is reported. After adding the G-rich DNA activator, the fluorescence intensity peaks in ~1 h and then starts to decay, where the decaying rate is much faster with light exposure. The lost fluorescence is recovered by adding NaBH4, suggesting that the bleaching is an oxidative process.Once activated, the G-rich activator can be removed while the AgNCs still maintain most of their fluorescence intensity. UV-vis spectroscopy suggests that new AgNC species are generated upon hybridization with the activator. The base sequence and length of the template DNA have also been varied, leading to different emission colors and color change after hybridization. G-rich aptamers can also serve as activators. Our results indicate that activation of the fluorescence by G-rich DNA could be a convenient method for biosensor development since the unstable NaBH4 is not required for the activation step.

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Evidence for Rod‐Shaped DNA‐Stabilized Silver Nanocluster Emitters

Cited by 181 publications

References 57 publications

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

Towards understanding of poly-guanine activated fluorescent silver nanoclusters

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