Evidence for Regulative Transactions between Cytoplasmic and Plastidial Translation by Euglena gracilis.

Brandt, Peter

doi:10.1016/s0044-328x(79)80113-0

Cited by 14 publications

(3 citation statements)

References 17 publications

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“…It has been shown, in vitro, by Brandt and Wiessner (1977) that chloroplast mRNA polymerase IV is completely inhibited at elevated temperatures. Furthermore, in vivo, several changes occur when E. oracilis is exposed to 34-35 ~ C, including a reduced incorporation of [14C]leucine into chloroplast proteins (Brandt 1981) and changes in the chloroplast protein pattern (Ortiz and Wilson 1988;Ortiz 1990). Under the same conditions we also observed a drastic decrease in chloroplast transcription, as measured by 32p incorporation into chloroplast RNAs (data not shown).…”

Section: Discussionsupporting

confidence: 52%

“…Several reports (Deng and Gruissem 1987;Mullet and Klein 1987) have shown that the transcriptional control of plastid genes during chloroplast development in higher plants might not be as important as previously believed, and that the stability of transcripts may play an appreciable role. To investigate the stability of the chloroplast transcripts we have used an elevated temperature (34-35 ~ C) which inhibits the chloroplast RNA polymerase (Brandt 1981(Brandt , 1988 thus providing an indirect but efficient way to follow chloroplast transcript stability. Etiolated cells were harvested and resuspended in resting medium for 12 h in darkness.…”

Section: Resultsmentioning

confidence: 99%

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Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Weiss

Houlné

Schantz

1992

Planta

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The expression of three chloroplastic genes, psbA (the gene for the reaction center D1 protein of PSII), psbC (the gene for the P700 apoprotein of PSI) and psaB (the gene for the intermediate antema of PSII), and a nuclear gene, cab (the gene for the chlorophyll a/bbinding protein), has been investigated during chloroplast development in Euglena gracilis Klebs. The polysomal fraction, mRNA stability and protein turnover were analysed under different conditions of cell greening. The results indicate that the main regulatory step for the nuclear and chloroplastic genes was at the translational level when greening of cells took place on a resting medium. When cell greening took place on a medium with balanced phosphate, carbon and nitrogen sources (nutritional medium), the main regulation occurred posttranscriptionally by mobilization of the transcripts onto the polysomes. These results indicate that in E. gracilis, for a given gene, regulation operates at different levels, and that although light is the principal effector in the regulation of the genes involved in chloroplast development, the effect of metabolites should also be considered. Interestingly, it appears that these various regulation levels are similar for the chloroplastic and nucleocytoplasmic compartments.

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Section: Discussionsupporting

confidence: 52%

Section: Resultsmentioning

confidence: 99%

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Weiss

Houlné

Schantz

1992

Planta

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“…Although the effects of organic substrates on chloroplast development has been extensively studied in Euglena and Chlorella Schwelitz et al, 1978a, b;Brandt, 1981Brandt, , 1988Schimpf et al, 1982;Schwartzbach and Schiff, 1983;Vannini, 1983;Rikin and Schwartzbach, 1989;Schwartzbach, 1990), was the only study, prior to this research, to examine this phenomenon in P.…”

Section: Uptake Of Dissolved Organic Substrates By Poterioochromonas mentioning

confidence: 99%

Physiological studies of phototrophy and heterotrophy in two algae with contrasting nutritional characteristics, Pyrenomonas salina (Cryptophyceae) and Poterioochromonas malhamensis (Chrysophyceae)

Lewitus¹

1990

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Diversification of isoelectric points from isolated chloroplasts during the cell cycle of Euglena gracilis, strain z

Brandt¹,

Ernst²

1982

Electrophoresis

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We describe a rapid method whereby isolated chloroplasts from Euglena gracilis can be separated in a Ficoll gradient according to their surface pK's by isoelectric focusing in one hour or less. The linear Ficoll gradient is made isotonic over the whole separation distance by addition of sucrose. No released proteins of the isolated chloroplasts are detectable after isoelectric focusing. Modifications of the chloroplast envelope during the cell cycle of Euglena gracilis are evident by alterations of their isoelectric points.

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Evidence for Regulative Transactions between Cytoplasmic and Plastidial Translation by Euglena gracilis.

Cited by 14 publications

References 17 publications

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Photocontrol of thylakoid protein synthesis in Euglena: differential post-transcriptional regulation depending on nutritional conditions

Physiological studies of phototrophy and heterotrophy in two algae with contrasting nutritional characteristics, Pyrenomonas salina (Cryptophyceae) and Poterioochromonas malhamensis (Chrysophyceae)

Diversification of isoelectric points from isolated chloroplasts during the cell cycle of Euglena gracilis, strain z

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