Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera

Sweet, Michael; Brown, Barbara E.; Dunne, R. P.; Singleton, Ian; Bulling, Mark T.

doi:10.1007/s00338-017-1572-y

Cited by 43 publications

(27 citation statements)

References 52 publications

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“…Transience appears to be a common trait among coral and E. diaphana microbiome members [80][81][82], and this was evident in the present study as the majority of ASVs detected in the control and heat-stressed samples at Day 0 were not seen at Day 14, and vice versa ( Figure 5). These transient bacteria, whilst comprising a small proportion of their bacterial communities (3.8-17.8%), were high in number, suggesting the presence of many species below the limit of detection that multiplied as conditions became favorable.…”

Section: Turnover Of Low-abundance Asvs Drive Shifts In Beta Diversitysupporting

confidence: 58%

The Effect of Thermal Stress on the Bacterial Microbiome of Exaiptasia diaphana

2019

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Coral bleaching linked to climate change has generated interest in the response of coral's bacterial microbiome to thermal stress. The sea anemone, Exaiptasia diaphana, is a popular coral model, but the response of its bacteria to thermal stress has been barely explored. To address this, we compared the bacterial communities of Great Barrier Reef (GBR) E. diaphana maintained at 26 • C or exposed to increasing temperature (26-33 • C) over two weeks. Communities were analyzed by metabarcoding of the bacterial 16S rRNA gene. Bleaching and Symbiodiniaceae health were assessed by Symbiodiniaceae cell density and dark-adapted quantum yield (F v /F m ), respectively. Significant bleaching and reductions in F v /F m occurred in the heat-treated anemones above 29 • C. Overall declines in bacterial alpha diversity in all anemones were also observed. Signs of bacterial change emerged above 31 • C. Some initial outcomes may have been influenced by relocation or starvation, but collectively, the bacterial community and taxa-level data suggested that heat was the primary driver of change above 32 • C. Six bacterial indicator species were identified as potential biomarkers for thermal stress. We conclude that the bacterial microbiome of GBR E. diaphana is generally stable until a thermal threshold is surpassed, after which significant changes occur. resistance or susceptibility during thermal stress have been observed [12][13][14]. This highlights the need for controlled, laboratory-based experiments to clarify the relationship between temperature-related bacterial community shifts and bleaching in cnidarians [15][16][17].The sea anemone, Exaiptasia diaphana, is a much-used model for coral symbiosis studies [18,19]. Its ability to propagate asexually for rapid growth of clonal populations, basic maintenance requirements and coral-like bleaching response to environmental stressors have seen it widely adopted by the research community and several clonal lines of different geographic origin and algal symbiont type have been established. However, studies using E. diaphana to explore cnidarian responses to heat stress have focused largely on aspects of the host-Symbiodiniaceae relationship [20][21][22][23][24][25], whilst its bacterial microbiome has been almost wholly neglected and data from only two studies are available.In a 2010 Master's thesis, E. diaphana of unspecified origin were exposed to temperature ramped from 26 • C to 31 • C over ten days, then held at 31 • C for four days [26]. No significant differences between the associated bacterial communities of control and treated anemones were detected across the study period. However, poor resolution of chosen molecular biology methods negatively impacted the findings. In addition, exposing the anemones to a maximum of 31 • C meant the anemones may not have been thermally stressed, and no measurements of algal cell density or photosynthetic performance were taken to assess their condition. Although the study revealed few insights, it is acknowledged as the first investigation...

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Section: Turnover Of Low-abundance Asvs Drive Shifts In Beta Diversitysupporting

confidence: 58%

The Effect of Thermal Stress on the Bacterial Microbiome of Exaiptasia diaphana

2019

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“…In recent years, the concept of “core microbiome” has been introduced in coral microbial ecology studies 10 , 26 , 37 , while the counterpart “stable microbiome” has also been used in related studies 26 , 37 , 38 . According to the statistical methods used for data analysis, coral-associated microbes can be assigned into “core/stable microbiome” and “transient/sporadic microbiome” 37 .…”

Section: Discussionmentioning

confidence: 99%

Exploring coral microbiome assemblages in the South China Sea

Lin

Tian

Zhou

et al. 2018

Sci Rep

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Coral reefs are significant ecosystems. The ecological success of coral reefs relies on not only coral-algal symbiosis but also coral-microbial partnership. However, microbiome assemblages in the South China Sea corals remain largely unexplored. Here, we compared the microbiome assemblages of reef-building corals Galaxea (G. fascicularis) and Montipora (M. venosa, M. peltiformis, M. monasteriata) collected from five different locations in the South China Sea using massively-parallel sequencing of 16S rRNA gene and multivariate analysis. The results indicated that microbiome assemblages for each coral species were unique regardless of location and were different from the corresponding seawater. Host type appeared to drive the coral microbiome assemblages rather than location and seawater. Network analysis was employed to explore coral microbiome co-occurrence patterns, which revealed 61 and 80 co-occurring microbial species assembling the Galaxea and Montipora microbiomes, respectively. Most of these co-occurring microbial species were commonly found in corals and were inferred to play potential roles in host nutrient metabolism; carbon, nitrogen, sulfur cycles; host detoxification; and climate change. These findings suggest that the co-occurring microbial species explored might be essential to maintain the critical coral-microbial partnership. The present study provides new insights into coral microbiome assemblages in the South China Sea.

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“…Three families (Pseudomonadaceae, Bacillaceae, and Moraxellaceae) are core members of the mucus microbiome of Mussimilia hispida 40 . In addition, Pseudomonadaceae are core members of the Coelastrea aspera microbiome 34 and associate with numerous other tropical stony corals, including Stylophora pistillata 41 , Pachyseris speciosa 37 , Fungia echinata 42 , Orbicella faveolata 43 , O. annularis 43 , and Astrangia poculata 44 , whose range extends from tropical to temperate regions. This family of bacteria also populates the microbiomes of tropical soft corals 45 , 46 , as well as cold-water gorgonians 19 .…”

Section: Discussionmentioning

confidence: 99%

“…The SAR324 clade, a group of Deltaproteobacteria, are capable of a variety of metabolic pathways 74 and thus could provide flexibility to their coral host. Streptococcus bacteria have been reported in other corals’ microbiomes 34 , 36 , 75 , but their function as part of the coral holobiont is not yet known. Both Staphylococcus 36 , 37 , 41 , 54 , 75 and Enterobacteriaceae 11 , 19 , 36 , 37 , 46 , 76 may act as opportunistic pathogens 14 , 77 , 78 in the corals whose microbiomes they inhabit.…”

Section: Discussionmentioning

confidence: 99%

Comparison of microbiomes of cold-water corals Primnoa pacifica and Primnoa resedaeformis, with possible link between microbiome composition and host genotype

Goldsmith

Kellogg

Morrison

et al. 2018

Sci Rep

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Cold-water corals provide critical habitats for a multitude of marine species, but are understudied relative to tropical corals. Primnoa pacifica is a cold-water coral prevalent throughout Alaskan waters, while another species in the genus, Primnoa resedaeformis, is widely distributed in the Atlantic Ocean. This study examined the V4-V5 region of the 16S rRNA gene after amplifying and pyrosequencing bacterial DNA from samples of these species. Key differences between the two species’ microbiomes included a robust presence of bacteria belonging to the Chlamydiales order in most of the P. pacifica samples, whereas no more than 2% of any microbial community from P. resedaeformis comprised these bacteria. Microbiomes of P. resedaeformis exhibited higher diversity than those of P. pacifica, and the two species largely clustered separately in a principal coordinate analysis. Comparison of P. resedaeformis microbiomes from samples collected in two submarine canyons revealed a significant difference between locations. This finding mirrored significant genetic differences among the P. resedaeformis from the two canyons based upon population genetic analysis of microsatellite loci. This study presents the first report of microbiomes associated with these two coral species.

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Evidence for rapid, tide-related shifts in the microbiome of the coral Coelastrea aspera

Cited by 43 publications

References 52 publications

The Effect of Thermal Stress on the Bacterial Microbiome of Exaiptasia diaphana

The Effect of Thermal Stress on the Bacterial Microbiome of Exaiptasia diaphana

Exploring coral microbiome assemblages in the South China Sea

Comparison of microbiomes of cold-water corals Primnoa pacifica and Primnoa resedaeformis, with possible link between microbiome composition and host genotype

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