Evidence for Persistent Expression of OX2 as a Necessary Component of Prolonged Renal Allograft Survival Following Portal Vein Immunization

The Same Immunoregulatory Molecules Contribute to Successful Pregnancy and Transplantation

“…In studies reported in detail elsewhere 35 we carried out in situ hybridization for CD200 mRNA in CBA/J × DBA/2 and CBA/J × BALB/c matings, while simultaneously staining adjacent sections of the tissue samples for fgl2 mRNA (fgl2 is a prothrombinase molecule whose expression is increased by certain Th1 cytokines implicated triggering pregnancy loss). While CD200 mRNA expression was up‐regulated following portal venous (pv) immunization and renal transplantation 31 , 41 in pregnant mice we observed no significant correlation between expression of the molecules fgl2 and CD200. Following cytokine treatment of pregnant animals, and prior to the onset of abortions, the proportion of fgl2 hi implantations was relatively unchanged, while the proportion of CD200 hi implants decreased dramatically, consistent with the notion that a major determinant of success or failure of fgl2 hi `at risk' implantations was the presence or absence of CD200.…”

“…Mixed leukocyte cultures were set up with 4 × 10 6 responder spleen cells and 2 × 10 6 mitomycin C‐treated stimulator spleen cells in 2 mL αF10 in 24‐well culture plates. Cytokine analysis was performed by enzyme‐linked immunosorbent assay (ELISA) 33 , 41 using supernatants harvested at 40 hr from these cultures. Cytotoxicity assays for cytoxic T lymphocyte (CTL) were performed at day 5, using 51 Cr‐labelled EL4 tumor target cells 30 …”

Transplant tolerance modifying antibody to CD200 receptor, but not CD200, alters cytokine production profile from stimulated macrophages

“…d) Cytokines in culture supernatant at 40 h. *p X 0.05 compared with first row; **p X 0.05 compared with all other groups. blocks the inhibition of alloreactivity both in vivo (leading to graft rejection) and in vitro [3,22]. In principle, since these studies were performed with whole Ig, it remained theoretically possible that cross-linking of surface CD200 could have occurred following treatment with anti-CD200, leading not to blocking of inhibition, but to delivery (via CD200 itself) of a stimulatory signal.…”

Co-infusion of donor adipose tissue-derived mesenchymal and hematopoietic stem cells helps safe minimization of immunosuppression in renal transplantation – single center experience

“…It believed to be related to switching from Th1 to Th2 cells with consequent changes in the cytokine milieu favoring levels of IL4 and IL10 thereby preventing rejection. 29,30 PBSC being rich in Tregs was infused in periphery. MSCs suppress the T lymphocyte proliferation induced by alloantigens, mitogens and anti-CD3 and anti-CD28 antibodies in vitro, in humans, baboons and mice.…”