Evidence for persistence of human parvovirus B19 DNA in bone marrow

Cassinotti, Pascal; Burtonboy, Guy; Fopp, M; Siegl, G.

doi:10.1002/(sici)1096-9071(199711)53:3<229::aid-jmv8>3.0.co;2-a

Cited by 144 publications

(43 citation statements)

References 24 publications

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“…Long-term persistence of B19V DNA in human tissue is a well known feature (Cassinotti et al, 1997;Eis-Hübinger et al, 2001;Söderlund et al, 1997;Wong et al, 2003). After acute infection, residual viral DNA can remain in tissue for decades or even lifelong ('bioportfolio') (Norja et al, 2006).…”

Section: Discussionmentioning

confidence: 51%

Simultaneous persistence of multiple genome variants of human parvovirus B19

Schneider

Höne

Tolba

et al. 2008

Journal of General Virology

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The species human parvovirus B19 (B19V) can be divided into three genotypes. In this study, we addressed the question as to whether infection of an individual is restricted to one genotype. As viral DNA is detectable in tissue for long times after acute infection, we examined 87 liver specimens from adults for the presence of B19V DNA. Fifty-nine samples were found to be positive, 32 of them for genotype 1, 27 for genotype 2 and four for genotype 3. In four samples, DNA of two genotypes was detected; samples from three individuals were positive for genotypes 1 and 2 and a sample from one individual was positive for genotypes 1 and 3. Surprisingly, significant sequence heterogeneity was observed at approximately 1 % of the nucleotides of the genotype 1 genomes from individuals with double genotype 1 and 2 infection. Controls using different enzymes for genome amplification and dilutions of the template verified that nucleotide heterogeneity was due to the presence of three or more genome variants of genotype 1. In summary, the evidence shows that individuals can be infected with two different genotypes, and B19V DNA can persist as a population of different genomes. The results may have implications for the understanding of the antiviral immune response and the development of vaccines against B19V.

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Section: Discussionmentioning

confidence: 51%

Simultaneous persistence of multiple genome variants of human parvovirus B19

Schneider

Höne

Tolba

et al. 2008

Journal of General Virology

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“…B19 viremia usually reaches a peak at days 7-9 after infection and is resolved by the development of an IgM-and IgG-antibody response starting a few days later. Despite the development and presence of B19-specific immune reactions, ϳ20% of all B19 infections show a prolonged state of viremia or viral persistence restricted to the synovial fluid, and viral genomes are detected in bone marrow or other organs, e.g., synovial tissue, liver, or myocardium, for several years after infection (4,8,12,13,19,27,(29)(30)(31)(32)(33). Compared with our age-matched control group and compared with healthy adult blood donors, who have been shown to contain B19 DNA in 7% and in 0.1% up to 0.6% of cases, respectively (34)(35)(36), viral genomes were detected in 35% of arthritis patients in the present study.…”

Section: Discussionmentioning

confidence: 99%

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Lehmann¹,

Knöll²,

Küster³

et al. 2003

Arthritis & Rheumatism

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Objective. To find further evidence of the association of parvovirus B19 infection with juvenile rheumatic diseases, and to get new insights into the immunopathogenesis of these diseases.Methods. Paired serum and synovial fluid samples from 74 children with rheumatic disease were analyzed with respect to their content of viral DNA and antibodies directed against the B19 viral proteins VP1, VP2, and NS1. Control sera from 124 children with noninflammatory bone diseases or growth retardation were also analyzed. The sequence of the viral DNA, amplified by polymerase chain reaction (PCR), was determined. IgG-complexed virus was isolated from sera and synovial fluid by adsorption to protein A beads. The amount of free virus versus immunocomplexed virus particles was determined by quantification of the viral genomes by quantitative PCR.Results. Twenty-six of the 74 patients (35%) had detectable amounts of parvovirus B19 DNA in the serum (n ‫؍‬ 22 [30%]) and/or the synovial fluid (n ‫؍‬ 16 [22%]), whereas only 9 of the 124 control sera (7%) were positive for the viral DNA (P < 0.0001). Forty-six patients (62%) had serum IgG against the structural proteins, indicating past infection with B19. NS1-specific antibodies were detected in sera from 29 patients (39%) and 27 controls (22%) (P < 0.001). In addition, 3 patients (4%) showed VP2-specific IgM. In 15 patients, viral DNA could be repeatedly detected in followup samples of serum and synovial fluid. Sequencing revealed lowdegree nucleotide variations that are in the range of genotype 1 of parvovirus B19. Immunocomplexed virus was present in varying amounts, both in the sera and in the synovial fluid samples.Conclusion. Parvovirus B19 is frequently found in serum or synovial fluid of children with rheumatism. The rate of persistent B19 infection in these patients is significantly higher than in age-matched controls.

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“…After primary infection, the erythroviral genomic DNA remains detectable in human tissues, in both symptomatic and asymptomatic subjects (24)(25)(26). Serodiagnostics (i.e., IgG seropositivity; IgG avidity, IgG epitope type specificity, and the absence of IgM ruling out recent primary infection) verified the specificity of the original findings and showed the DNA persistence in synovium to be long (24,27).…”

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confidence: 99%

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Norja

Hokynar

Aaltonen³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

244

216

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Human erythrovirus is a minute, single-stranded DNA virus causing many diseases, including erythema infectiosum, arthropathy, and fetal death. After primary infection, the viral genomes persist in solid tissues. Besides the prototype, virus type 1, two major variants (virus types 2 and 3) have been identified recently, the clinical significance and epidemiology of which are mostly unknown. We examined 523 samples of skin, synovium, tonsil, or liver (birth year range, 1913-2000), and 1,640 sera, by qualitative and quantitative molecular assays for the DNA of human erythroviruses. Virus types 1 and 2 were found in 132 (25%) and 58 (11%) tissues, respectively. DNA of virus type 1 was found in all age groups, whereas that of type 2 was strictly confined to those subjects born before 1973 (P < 0.001). Correspondingly, the sera from the past two decades contained DNA of type 1 but not type 2 or 3. Our data suggest strongly that the newly identified human erythrovirus type 2 as well as the prototype 1 circulated in Northern and Central Europe in equal frequency, more than half a century ago, whereafter type 2 disappeared from circulation. Type 3 never attained wide occurrence in this area during the past >70 years. The erythrovirus DNA persistence in human tissues is lifelong and represents a source of information about our past, the Bioportfolio, which, at the individual level, provides a registry of one's infectious encounters, and at the population level, a database for epidemiological and phylogenetic analyses.epidemiology ͉ gene therapy ͉ parvovirus ͉ phylogeny ͉ single-stranded DNA

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Evidence for persistence of human parvovirus B19 DNA in bone marrow

Cited by 144 publications

References 24 publications

Simultaneous persistence of multiple genome variants of human parvovirus B19

Simultaneous persistence of multiple genome variants of human parvovirus B19

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

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