Evidence for neurokinin-3 receptor-mediated tachykinin release in the guinea-pig ileum

Guard, Steven; Watson, Steve P.

doi:10.1016/0014-2999(87)90398-0

Cited by 60 publications

(33 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results that SP-induced contractions were inhibited by atropine and tetrodotoxin to a large and nearly complete extent, respectively, in the segment prepa ration indicate that these contractions are for the most part neurogenic. There is ample evidence that activation of NK3-receptors causes a release of acetylcholine and also possibly tachykinins from the myenteric plexus neu rons in guinea pig ileum (5,19,20). SP may indirectly produce contractions in the circular muscle through the activation of NK3-receptors, thereby releasing endo genous acetylcholine and tachykinins in the segment preparation.…”

Section: Discussionmentioning

confidence: 99%

Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.

Suzuki¹,

Muramatsu²,

Gomi³

1994

Jpn.J.Pharmacol

View full text Add to dashboard Cite

ABSTRACT-Actions of substance P (SP, 10-9 to 10-6 M), neurokinin A (NKA, 10-9 to 10-6 M) and neurokinin B (NKB, 10-10 to 10-6 M) in the circular muscle of guinea pig ileum were investigated in segment and strip preparations, in which methacholine produced similar contractions. In the segment preparations, three tachykinins produced repeatedly occurring twitch-like contractions. Their efficacies were similar with the same maximal contractions, but their potencies were different (NKB > NKA = SP). Latency (38 sec) was observed before the initiation of contractions in response to NKA, but not to SP or NKB. Atropine (10-6 M) and tetrodotoxin (3 x 10-'M) did not affect NKA-induced contractions, but inhibited SP and NKB induced contractions; the dose-response curves for SP and NKB were rightwardly shifted by atropine. The treatment with atropine brought out latency in the responses for NKB. In the strip preparations, SP did not substantially induce contractions, but NKA and NKB produced twitch-like contractions after latent periods of 28 and 36 sec, respectively. The efficacy of NKA was similar to that in segment preparations, while that of NKB was much lower in strip preparations. Unlike in segment preparations, atropine did not inhibit contrac tions induced by the two tachykinins in strip preparations. These results suggest that tachykinins induce con tractions through myogenic and neurogenic mechanisms, the latter of which may be inoperative in strip preparations.

show abstract

Section: Discussionmentioning

confidence: 99%

Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.

Suzuki¹,

Muramatsu²,

Gomi³

1994

Jpn.J.Pharmacol

View full text Add to dashboard Cite

show abstract

“…There is evidence that NK1 receptors mediate the direct response of muscle cells while NK3 receptors activate intramural effector neurones which in turn release acetylcholine and possibly endogenous TKs (Kilbinger et al, 1986;Laufer et al, 1986;Guard & Watson, 1987). In spite of its importance for the genesis of propulsive activity and persistalsis, the effects of TKs on the circular muscle have been studied less (Holzer et al, 1980;Costa et al, 1985a,b).…”

Section: Introductionmentioning

confidence: 99%

Tachykininergic transmission to the circular muscle of the guinea‐pig ileum: evidence for the involvement of NK₂ receptors

Barthó

Santicioli

Patacchini

et al. 1992

British J Pharmacology

View full text Add to dashboard Cite

1 The effect of newly developed, receptor-selective tachykinin antagonists (GR 71,251 for NK1 receptors, MEN 10,376 and L 659,877 for NK2 receptors) on noncholinergic transmission to the circular muscle of the guinea-pig ileum has been investigated. 2 In circular muscle strips of the ileum, electrical field stimulation in the presence of atropine (2 jM) and apamin (0.1 jAM) evoked a complex motor response. The tonic primary contraction in this response was reduced by GR 71,251 (10 jM) and MEN 10,376 (3-10 I1M) but not by L 659,877 (up to 10 jM). The presence of apamin was necessary in this experimental arrangement to unmask an atropine-resistant primary contraction, sensitive to tachykinin antagonists. The motor response was abolished by tetrodotoxin.3 In circular strips of the ileum GR 71,251 (10 jM) inhibited the tonic contraction produced by [Sar9] substance P sulphone, a selective NK1 receptor agonist but not that produced by [,B Alal] neurokinin A (4-10), a selective NK2 receptor agonist. By contrast, MEN 10,376 antagonized the effect of the NK2 agonist while leaving the response to the NK, agonist unaffected. 4 In whole segments of the ileum, distension of the gut wall by an intraluminal balloon placed at about 1 cm from the point of recording of mechanical activity of the circular muscle produced atropinesensitive phasic contractions (ascending enteric reflex). In the presence of atropine (2 jiM), a noncholinergic response was elicited, which required larger volumes of distension that the cholinergic one. The atropine-resistant ascending enteric reflex was enhanced by apamin (0.1 j1M) and abolished by tetrodotoxin, either in the presence or absence of apamin. 5 MEN 10,376 (3-lOM) inhibited the atropine-resistant ascending enteric reflex in the presence of apamin while GR 71,251 or L 659,877 (10 lM each) were ineffective. MEN 10,376 inhibited the atropine-resistant ascending enteric reflex to a larger extent in the absence than in the presence of apamin and also slightly inhibited the ascending enteric reflex in the absence of atropine. 6 These findings provide evidence for an involvement of NK2 tachykinin receptors in excitatory transmission to the circular muscle of the guinea-pig ileum. NK2 receptors are also involved in the physiological-like circular muscle activation produced by stimulation of intramural neuronal pathways which subserve the atropine-resistant ascending enteric reflex.

show abstract

“…In the longitudinal muscle, at least two TK receptors are present: one receptor (NK1) mediates the direct response of muscle cells while the other (NK3) activates intramural effector neurones which in turn release acetylcholine and possibly endogenous TKs (Kilbinger et al 1986;Laufer et al, 1986;Guard & Watson, 1987). Some studies have also suggested that a third type of TK receptor (NK2) may mediate the contractile response of the guinea-pig ileal longitudinal muscle (Jacoby et al, 1986;Dion et al, 1987) while other investigations have excluded this possibility (Laufer et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Tachykinin receptors in the circular muscle of the guinea‐pig ileum

Maggi

Patacchini

Giachetti

et al. 1990

British J Pharmacology

View full text Add to dashboard Cite

The response to the selective NK2-agonist was unchanged after desensitization of NK1-or NK3-receptors.6 The response to the NK2-selective agonist was strongly inhibited by [Tyr5,8'9, Argl0]-NKA(4-10) (MEN 10,207) a selective NK2-receptor antagonist which did not modify the response to the NK1-selective agonist. 7 Our findings indicate that all the three known types of tachykinin receptors mediate the contractile response of the circular muscle of the guinea-pig ileum to peptides of this family. The response to activation of NK3-receptors is totally neurogenic and partially mediated by endogenous acetylcholine, the response to activation of NK1-receptors is partly neurogenic and largely myogenic and the response to activation of NK2-receptors is totally myogenic.

show abstract

Evidence for neurokinin-3 receptor-mediated tachykinin release in the guinea-pig ileum

Cited by 60 publications

References 7 publications

Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.

Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.

Tachykininergic transmission to the circular muscle of the guinea‐pig ileum: evidence for the involvement of NK₂ receptors

Tachykinin receptors in the circular muscle of the guinea‐pig ileum

Contact Info

Product

Resources

About

Evidence for neurokinin-3 receptor-mediated tachykinin release in the guinea-pig ileum

Cited by 60 publications

References 7 publications

Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.

Tachykinin-Induced Contractions in the Circular Muscle of Guinea Pig Ileum.

Tachykininergic transmission to the circular muscle of the guinea‐pig ileum: evidence for the involvement of NK2 receptors

Tachykinin receptors in the circular muscle of the guinea‐pig ileum

Contact Info

Product

Resources

About

Tachykininergic transmission to the circular muscle of the guinea‐pig ileum: evidence for the involvement of NK₂ receptors