Evidence for mixed sexual and asexual reproduction in the rare European mycoheterotrophic orchid<i>Epipogium aphyllum</i>, Orchidaceae (ghost orchid)

Krawczyk, Emilia; Rojek, Joanna; Kowalkowska, Agnieszka K.; Kapusta, Małgorzata; Znaniecka, Joanna; Minasiewicz, Julita

doi:10.1093/aob/mcw084

Cited by 16 publications

(15 citation statements)

References 39 publications

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“…The enlargement of ovaries, which we also noted in the tested plants, can take place due to successful pollination and sometimes in emasculated flowers and flowers isolated from pollination. Hence, the enlargement of the ovary without pollination may be indicative of a programmed phenomenon ( Krawczyk et al, 2016 ). The genesis and fate of observed C. × fred-mulleri young seed capsules and embryos remains to be determined, together with consideration of the environmental factors (e.g., pollination limitations) and mechanisms which decrease seed formation efficiency.…”

Section: Discussionmentioning

confidence: 99%

First Guatemalan record of natural hybridisation between Neotropical species of the Lady’s Slipper orchid (Orchidaceae, Cypripedioideae)

Szlachetko

Kolanowska

Muller³

et al. 2017

PeerJ

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The first natural hybrid in the section Irapeana of the orchid genus Cypripedium is described and illustrated based on Guatemalan material. A molecular evaluation of the discovery is provided. Specimens with intermediate flowers between C. irapeanum and C. dickinsonianum within ITS and Xdh sequences have the signal sequence of both these species. The analysis of plastid sequences indicated that the maternal line is C. irapeanum. Information about the ecology, embryology and conservation status of the novelty is given, together with a distribution map of its parental species, C. irapeanum and C. dickinsonianum. A discussion of the hybridization between Cypripedium species is presented. The potential hybrid zones between the representatives of Cypripedium section Irapeana which were estimated based on the results of ecological niche modeling analysis are located in the Maya Highlands (C. dickinsonianum and C. irapeanum) and the eastern part of Southern Sierra Madre (C. molle and C. irapeanum). Moreover, all three Cypripedium species could inhabit Cordillera Neovolcánica according to the obtained models; however, it should be noticed that this region is well-distanced from the edges of the known geographical range of C. molle.

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Section: Discussionmentioning

confidence: 99%

First Guatemalan record of natural hybridisation between Neotropical species of the Lady’s Slipper orchid (Orchidaceae, Cypripedioideae)

Szlachetko

Kolanowska

Muller³

et al. 2017

PeerJ

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“…Samples were prepared for the above mentioned observations in accordance with previously described procedures (i.e. Kowalkowska et al 2010; Krawczyk et al 2016; Święczkowska and Kowalkowska 2015; Naczk et al 2018). The sections were examined in a Tecnai G2 Spirit BioTwin FEI transmission electron microscope (Laboratory of Electron Microscopy, University of Gdańsk, Poland) at an accelerating voltage of 120 kV.…”

Section: Methodsmentioning

confidence: 99%

Labellum structure of Bulbophyllum echinolabium J.J. Sm. (section Lepidorhiza Schltr., Bulbophyllinae Schltr., Orchidaceae Juss.)

et al. 2019

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This micromorphological, chemical and ultrastructural study is a continuation of research conducted on the section Lepidorhiza . The Bulbophyllum echinolabium flowers comprised features that characterize a sapromyophilous syndrome, having large, glistening parts that emit an intense scent of rotten meat. The secretory activity was described in the hypochile (nectary in longitudinal groove and in the prickles) and the epichile (putative osmophore). The ultrastructural studies revealed a dense cytoplasm in the epidermis and subepidermal tissue with large nuclei and numerous mitochondria, the profiles of SER and RER, and dictyosomes. Large amounts of heterogeneous residues of secreted material (possibly phenolic) were present on the cuticle surface, similar to the unusual prominent periplasmic space with flocculent secretory material. The chemical analysis (GC/MS) of the scent profile of lips comprised carbohydrates and their derivatives (29.55% of all compounds), amino acids (1.66%), lipids (8.04%) and other organic compounds (60.73%). A great number of identified compounds are Diptera attractants (mainly Milichiidae, Tephritidae, Drosophilidae, Muscidae, Sarcophagidae, Tachinidae). The examination of visual and olfactory features indicates correlation between colour of flowers and the type of olfactory mimicry, where a dark colour labellum emits strong smell of rotten waste.

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“…Flower buds and flowers were prepared as described by Krawczyk et al (2016) . After dehydration, material was embedded in Steedman’s wax and sectioned at 2–5 µm.…”

Section: Methodsmentioning

confidence: 99%

Establishing the cell biology of apomictic reproduction in diploid Boechera stricta (Brassicaceae)

Rojek

Kapusta

Kozieradzka-Kiszkurno

et al. 2018

Annals of Botany

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Background and aimsIn the Brassicaceae family, apomictic development is characteristic of the genus Boechera. Hybridization, polyploidy and environmental adaptation that arose during the evolution of Boechera may serve as (epi)genetic regulators of apomictic initiation in this genus. Here we focus on Boechera stricta, a predominantly diploid species that reproduces sexually. However, apomictic development in this species has been reported in several studies, indicating non-obligate sexuality.MethodsA progressive investigation of flower development was conducted using three accessions to assess the reproductive system of B. stricta. We employed molecular and cyto-embryological identification using histochemistry, transmission electron microscopy and Nomarski and epifluorescence microscopy.Key ResultsData from internal transcribed spacer (ITS) and chloroplast haplotype sequencing, in addition to microsatellite variation, confirmed the B. stricta genotype for all lines. Embryological data indicated irregularities in sexual reproduction manifested by heterochronic ovule development, longevity of meiocyte and dyad stages, diverse callose accumulation during meiocyte-to-gametophyte development, and the formation of triads and tetrads in several patterns. The arabinogalactan-related sugar epitope recognized by JIM13 immunolocalized to one or more megaspores. Furthermore, pollen sterility and a high frequency of seed abortion appeared to accompany reproduction of the accession ES512, along with the initiation of parthenogenesis. Data from flow cytometric screening revealed both sexual and apomictic seed formation.ConclusionThese results imply that B. stricta is a species with an underlying ability to initiate apomixis, at least with respect to the lines examined here. The existence of apomixis in an otherwise diploid sexual B. stricta may provide the genomic building blocks for establishing highly penetrant apomictic diploids and hybrid relatives. Our findings demonstrate that apomixis per se is a variable trait upon which natural selection could act.

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Evidence for mixed sexual and asexual reproduction in the rare European mycoheterotrophic orchidEpipogium aphyllum, Orchidaceae (ghost orchid)

Cited by 16 publications

References 39 publications

First Guatemalan record of natural hybridisation between Neotropical species of the Lady’s Slipper orchid (Orchidaceae, Cypripedioideae)

First Guatemalan record of natural hybridisation between Neotropical species of the Lady’s Slipper orchid (Orchidaceae, Cypripedioideae)

Labellum structure of Bulbophyllum echinolabium J.J. Sm. (section Lepidorhiza Schltr., Bulbophyllinae Schltr., Orchidaceae Juss.)

Establishing the cell biology of apomictic reproduction in diploid Boechera stricta (Brassicaceae)

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