Evidence for Methylerythritol Pathway (MEP) Contributions to Zerumbone Biosynthesis as Revealed by Expression Analysis of Regulatory Genes and Metabolic Inhibitors Studies

Nair, Aswati R.; Divakaran, Keerthi; Pillai, Padmesh P.

doi:10.1007/s11105-020-01202-5

Cited by 7 publications

(2 citation statements)

References 47 publications

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“…The mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathways are two independent, compartmentally separated pathways for terpenoid formation [ 22 , 23 , 24 , 25 ]. The MEP pathway is mainly responsible for the biosynthesis of mono- and diterpenes, while the MVA pathway mainly produces sesquiterpenes, sterols, and triterpenes in plants [ 1 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Wei

Yang

et al. 2023

IJMS

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Terpenes, especially volatile terpenes, are important components of tea aroma due to their unique scents. They are also widely used in the cosmetic and medical industries. In addition, terpene emission can be induced by herbivory, wounding, light, low temperature, and other stress conditions, leading to plant defense responses and plant–plant interactions. The transcriptional levels of important core genes (including HMGR, DXS, and TPS) involved in terpenoid biosynthesis are up- or downregulated by the MYB, MYC, NAC, ERF, WRKY, and bHLH transcription factors. These regulators can bind to corresponding cis-elements in the promoter regions of the corresponding genes, and some of them interact with other transcription factors to form a complex. Recently, several key terpene synthesis genes and important transcription factors involved in terpene biosynthesis have been isolated and functionally identified from tea plants. In this work, we focus on the research progress on the transcriptional regulation of terpenes in tea plants (Camellia sinensis) and thoroughly detail the biosynthesis of terpene compounds, the terpene biosynthesis-related genes, the transcription factors involved in terpene biosynthesis, and their importance. Furthermore, we review the potential strategies used in studying the specific transcriptional regulation functions of candidate transcription factors that have been discriminated to date.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Wei

Yang

et al. 2023

IJMS

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“…The terpene synthesis pathway is categorized into the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. The MVA pathway occurs within the cytoplasm, whereas the MEP pathway takes place in the plastid [23]. The key rate-limiting enzyme of the MEP pathway is the extensively studied 1-deoxy-D-xylulose-5-phosphate synthase (DXS) [24].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Volatile Organic Compounds in Five Celery (Apium graveolens L.) Cultivars with Different Petiole Colors by HS-SPME-GC-MS

Sun,

Li,

et al. 2023

IJMS

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Celery (Apium graveolens L.) is an important vegetable crop cultivated worldwide for its medicinal properties and distinctive flavor. Volatile organic compound (VOC) analysis is a valuable tool for the identification and classification of species. Currently, less research has been conducted on aroma compounds in different celery varieties and colors. In this study, five different colored celery were quantitatively analyzed for VOCs using HS-SPME, GC-MS determination, and stoichiometry methods. The result revealed that γ-terpinene, d-limonene, 2-hexenal,-(E)-, and β-myrcene contributed primarily to the celery aroma. The composition of compounds in celery exhibited a correlation not only with the color of the variety, with green celery displaying a higher concentration compared with other varieties, but also with the specific organ, whereby the content and distribution of volatile compounds were primarily influenced by the leaf rather than the petiole. Seven key genes influencing terpenoid synthesis were screened to detect expression levels. Most of the genes exhibited higher expression in leaves than petioles. In addition, some genes, particularly AgDXS and AgIDI, have higher expression levels in celery than other genes, thereby influencing the regulation of terpenoid synthesis through the MEP and MVA pathways, such as hydrocarbon monoterpenes. This study identified the characteristics of flavor compounds and key aroma components in different colored celery varieties and explored key genes involved in the regulation of terpenoid synthesis, laying a theoretical foundation for understanding flavor chemistry and improving its quality.

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Secondary Metabolite Production from Roots/Rhizomes: Prospects and Challenges in Developing Differentiated Cultures Using the Plant’s Hidden Half

Nair

Harsha

Harshitha

et al. 2022

Phytochemical Genomics

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Evidence for Methylerythritol Pathway (MEP) Contributions to Zerumbone Biosynthesis as Revealed by Expression Analysis of Regulatory Genes and Metabolic Inhibitors Studies

Cited by 7 publications

References 47 publications

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Biosynthesis and the Transcriptional Regulation of Terpenoids in Tea Plants (Camellia sinensis)

Characterization of Volatile Organic Compounds in Five Celery (Apium graveolens L.) Cultivars with Different Petiole Colors by HS-SPME-GC-MS

Secondary Metabolite Production from Roots/Rhizomes: Prospects and Challenges in Developing Differentiated Cultures Using the Plant’s Hidden Half

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