Evidence for involvement of 2 types of reaction in glycolate formation during photosynthesis in isolated spinach chloroplasts

Effects of manganese ions and magnesium ions on the activity of soya-bean ribulose bisphosphate carboxylase/oxygenase

“…This higher cation concentration is reflected in the higher Ka(catIon) for oxygenase than for carboxylase activities. Our data also provide a possible explanation for the biphasic response of glycollate synthesis by isolated chloroplasts to 02 concentration observed by Eichenbusch & Beck (1973). The low rate of synthesis at low 02 concentrations would be supported by exogenously supplied Mn2+ whereas the higher rate at high 02 concentrations would be due to the expression of activity with exogenous and endogenous Mg2+ (see Fig.…”

Photoexcretion and Fate of Glycolate in a Hot Spring Cyanobacterial Mat

“…1985, 1-103, p. 163). Other investigators have demonstrated that high oxygen and low carbon dioxide partial pressures enhance glycolate biosynthesis and excretion of the photoassimilated carbon by a variety of phototrophic organisms (3,5,12,13,23,26). The extracellular photosynthate detected in samples from the Mushroom Spring microbial mat was likely produced by the cyanobacterium S. lividus, because anoxygenic photosynthesis by the other known mat phototroph, Chloroflexus aurantiacus, is probably prevented by oxygen exclusion of sulfide from the photic zone at the light intensities used in our experiments (18).…”

Torn Between Nature and Lab: A Dying Breed of Plant Scientists?

“…With our chloroplasts we could show that a minor amount of the early photosynthetic product glycolate resulted from the oxidation of "activated," i.e., transketolase-bound glycolaldehyde by an unknown oxidant, whereas the bulk of glycolate originates in an oxygen dependent reaction from Ribulose-bisphosphate (Eickenbusch and Beck 1973;Eickenbusch et al 1975;). This coincided with Tolbert's group (Lorimer et al 1973) publishing the oxygenase reaction of RUBISCO.…”