Evidence for induced DNA bending by the yeast zinc cluster protein PUT3

Hoffmann, Pascal; Schepartz, Alanna

doi:10.1016/s0960-894x(97)00355-7

Cited by 2 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2B). The strong DNase I-hypersensitive site in the C 2 binding site may be due to Rgt1-induced DNA bending, since poly(A) tracts can cause DNA bending (23) and some Zn cluster proteins significantly bend DNA (16).…”

Section: Resultsmentioning

confidence: 99%

Specificity and Regulation of DNA Binding by the Yeast Glucose Transporter Gene Repressor Rgt1

Kim

Polish

Johnston

2003

Molecular and Cellular Biology

118

165

View full text Add to dashboard Cite

Rgt1 is a glucose-responsive transcription factor that binds to the promoters of several HXT genes encoding glucose transporters in Saccharomyces cerevisiae and regulates their expression in response to glucose. Rgt1 contains a Zn 2 Cys 6 binuclear cluster responsible for DNA binding. Most proteins that contain this sequence motif bind as dimers to regularly spaced pairs of the sequence CGG. However, there are no CGG pairs with regular spacing in promoters of genes regulated by Rgt1, suggesting that Rgt1 binds as a monomer to CGG or to another sequence. We identified the Rgt1 consensus binding site sequence 5-CGGANNA-3, multiple copies of which are present in all HXT promoters regulated by Rgt1. Rgt1 binds in vivo to multiple sites in the HXT3 promoter in a nonadditive, synergistic manner, leading to synergistic repression of HXT3 transcription. We show that glucose inhibits the DNA-binding ability of Rgt1, thereby relieving repression of HXT gene expression. This regulation of Rgt1 DNA-binding activity is caused by its glucose-induced phosphorylation: the hyperphosphorylated Rgt1 present in cells growing on high levels of glucose does not bind DNA in vivo or in vitro; dephosphorylation of this form of Rgt1 in vitro restores its DNA-binding ability. Furthermore, an altered Rgt1 that functions as a constitutive repressor remains hypophosphorylated when glucose is added to cells and binds DNA under these conditions. These results suggest that glucose regulates the DNA-binding ability of Rgt1 by inducing its phosphorylation.

show abstract

Section: Resultsmentioning

confidence: 99%

Specificity and Regulation of DNA Binding by the Yeast Glucose Transporter Gene Repressor Rgt1

Kim

Polish

Johnston

2003

Molecular and Cellular Biology

118

165

View full text Add to dashboard Cite

show abstract

“…Some other Zn(II) 2 Cys 6 binuclear cluster proteins have been shown to significantly bend DNA upon binding, e.g. Put3p bends DNA by approximately 40° [27,28], and a similar apparent flexure angle has been calculated for Leu3p [G. Kohlhaw, personal communication]. How is h-IPM able to convert Leu3p into a transcriptional activator?…”

Section: Leucine Biosynthesismentioning

confidence: 94%

Molecular basis of nutrient-controlled gene expression in Saccharomyces cerevisiae

Reece

2000

CMLS, Cell. Mol. Life Sci.

View full text Add to dashboard Cite

The ability of a unicellular organism to alter patterns of gene expression in response to nutrient availability is essential to its survival in a changing environment. How is the cell able to identify individual metabolites amongst a myriad of other similar molecules, and convert the information of its presence into a concerted change in the transcription of the genes required for the response to that metabolite? There is increasing evidence that the activity of transcription factors can be influenced directly by interaction with metabolites. A variety of mechanisms have been identified by which this type of gene regulation by small molecules can occur.

show abstract

Evidence for induced DNA bending by the yeast zinc cluster protein PUT3

Cited by 2 publications

References 17 publications

Specificity and Regulation of DNA Binding by the Yeast Glucose Transporter Gene Repressor Rgt1

Specificity and Regulation of DNA Binding by the Yeast Glucose Transporter Gene Repressor Rgt1

Molecular basis of nutrient-controlled gene expression in Saccharomyces cerevisiae

Contact Info

Product

Resources

About