Evidence for increased tissue androgen sensitivity in neurturin knockout mice

Simanainen, Ulla; Gao, Yan; Desai, Reena; Jimenez, Mark; Spaliviero, Jenny; Keast, Janet R.; Handelsman, David J.

doi:10.1530/joe-13-0056

Cited by 4 publications

(2 citation statements)

References 65 publications

(87 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These sweeps in domestic pigs (EUD and ASD) were significantly enriched with GO terms related to multiple developmental processes of bones, teeth and nervous system (Table S1&S2).These terms comprise multiple gene candidates related to height ( PLAGJ , NCPAG , PENK , RPS20 and LYN in EUD; Fig. 3a; LEMD3 and UPKJ in ASD) in pigs (37, 38) and cattle (39, 40), nervous system development and maintenance ( NRTN, SEMA3C, PLXNCJ, AAKJ, RAB35, FRS2 ) (41-52) as well as genes directly influencing behaviour ( i.e. aggressiveness and feeding; APBA2 , MC4R , RCANJ , BAJPA3 ) (53-60).…”

Section: Resultsmentioning

confidence: 99%

Analyses of Eurasian wild and domestic pig genomes reveals long-term gene-flow and selection during domestication

Frantz

Schraiber

Madsen

et al. 2014

Preprint

View full text Add to dashboard Cite

Traditionally, the process of domestication is assumed to be initiated by people, involve few individuals and rely on reproductive isolation between wild and domestic forms. However, an emerging zooarcheological consensus depicts animal domestication as a long-term process without reproductive isolation or strong intentional selection. Here, we ask whether pig domestication followed a traditional linear model, or a complex, reticulate model as predicted by zooarcheologists. To do so, we fit models of domestication to whole genome data from over 100 wild and domestic pigs. We found that the assumptions of traditional models, such as reproductive isolation and strong domestication bottlenecks, are incompatible with the genetic data and provide support for the zooarcheological theory of a complex domestication process. In particular, gene-flow from wild to domestic pigs was a ubiquitous feature of the domestication of pigs. In addition, we show that despite gene-flow, the genomes of domestic pigs show strong signatures of selection at loci that affect behaviour and morphology. Specifically, our results are consistent with independent parallel sweeps in two independent domestication areas (China and Anatolia) at loci linked to morphological traits. We argue that recurrent selection for domestic traits likely counteracted the homogenising effect of gene-flow from wild boars and created "islands of domestication" in the genome. Overall, our results suggest that genomic approaches that allow for more complex models of domestication to be embraced should be employed. The results from these studies will have significant ramifications for studies that attempt to infer the origin of domesticated animals.

show abstract

Section: Resultsmentioning

confidence: 99%

Analyses of Eurasian wild and domestic pig genomes reveals long-term gene-flow and selection during domestication

Frantz

Schraiber

Madsen

et al. 2014

Preprint

View full text Add to dashboard Cite

show abstract

“…1 week prior to transplantation, some of the 7 weeks old nude mice were castrated and implanted subcutaneously with a 0.5–1.0 cm silicon tube with an internal diameter of 2 mm (Kaneka Medix Corp., Japan) containing testosterone (25 mg/ml; Wako, Japan), dihydrotestosterone (DHT; 100 mg/ml; Sigma-Aldrich), or sesame oil alone. In the present study, we used the almost same or higher dose of T/DHT than the previous experiments of androgen-treatment with silicon tube into mice [79–81]. The testosterone levels in each male host group were estimated as the weight of their seminal vesicles [36] (S1 Fig).…”

Section: Methodsmentioning

confidence: 99%

Molecular and genetic characterization of partial masculinization in embryonic ovaries grafted into male nude mice

et al. 2019

View full text Add to dashboard Cite

In most of mammalian embryos, gonadal sex differentiation occurs inside the maternal uterus before birth. In several fetal ovarian grafting experiments using male host mice, an experimental switch from the maternal intrauterine to male-host environment gradually induces partial masculinization of the grafted ovaries even under the wild-type genotype. However, either host-derived factors causing or molecular basis underlying this masculinization of the fetal ovaries are not clear. Here, we demonstrate that ectopic appearance of SOX9-positive Sertoli cell-like cells in grafted ovaries was mediated by the testosterone derived from the male host. Neither Sox8 nor Amh activity in the ovarian tissues is essential for such ectopic appearance of SOX9-positive cells. The transcriptome analyses of the grafted ovaries during this masculinization process showed early downregulation of pro-ovarian genes such as Irx3 , Nr0b1 / Dax1 , Emx2 , and Fez1 / Lzts1 by days 7–10 post-transplantation, and subsequent upregulation of several pro-testis genes, such as Bhlhe40 , Egr1/2 , Nr4a2 , and Zc3h12c by day 20, leading to a partial sex reversal with altered expression profiles in one-third of the total numbers of the sex-dimorphic pre-granulosa and Sertoli cell-specific genes at 12.5 dpc. Our data imply that the paternal testosterone exposure is partially responsible for the sex-reversal expression profiles of certain pro-ovarian and pro-testis genes in the fetal ovaries in a temporally dependent manner.

show abstract

Evidence of long-term gene flow and selection during domestication from analyses of Eurasian wild and domestic pig genomes

et al. 2015

View full text Add to dashboard Cite

Traditionally, the process of domestication is assumed to be initiated by humans, involve few individuals and rely on reproductive isolation between wild and domestic forms. We analyzed pig domestication using over 100 genome sequences and tested whether pig domestication followed a traditional linear model or a more complex, reticulate model. We found that the assumptions of traditional models, such as reproductive isolation and strong domestication bottlenecks, are incompatible with the genetic data. In addition, our results show that, despite gene flow, the genomes of domestic pigs have strong signatures of selection at loci that affect behavior and morphology. We argue that recurrent selection for domestic traits likely counteracted the homogenizing effect of gene flow from wild boars and created 'islands of domestication' in the genome. Our results have major ramifications for the understanding of animal domestication and suggest that future studies should employ models that do not assume reproductive isolation.

show abstract

Evidence for increased tissue androgen sensitivity in neurturin knockout mice

Cited by 4 publications

References 65 publications

Analyses of Eurasian wild and domestic pig genomes reveals long-term gene-flow and selection during domestication

Analyses of Eurasian wild and domestic pig genomes reveals long-term gene-flow and selection during domestication

Molecular and genetic characterization of partial masculinization in embryonic ovaries grafted into male nude mice

Evidence of long-term gene flow and selection during domestication from analyses of Eurasian wild and domestic pig genomes

Contact Info

Product

Resources

About