Evidence for extrachromosomal elements in Lactobacillus

Chassy, Bruce M.; Gibson, Evelyn M.; Giuffrida, Alessandro

doi:10.1128/jb.127.3.1576-1578.1976

Cited by 77 publications

(24 citation statements)

References 5 publications

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“…To isolate DNA from lactobacilli, 5 ml of an overnight culture of each strain grown in MRS broth were diluted with 10 ml pre-warmed MRS broth. After about 4 h of incubation at 37°C, the bacterial cells were harvested by centrifugation and the chromosomal DNA was isolated as described by Chassy et al (1976).…”

Section: Genomic Dna Extractionmentioning

confidence: 99%

Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences

et al. 2002

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Aims: Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment. Methods and Results: Thirty‐five Lactobacillus strains from women from seven countries were isolated, and sequences from 16S rRNA genes were determined and compared with existing data in GenBank. A phylogenetic tree was achieved using the Neighbour‐Joining method based on the analysis of 1465 nucleotides. The results showed that most vaginal isolates were L. crispatus, L. jensenii and L. gasseri. Some were L. vaginalis, L. fermentum, L. mucosae, L. paracasei and L. rhamnosus. Two isolates from a native American woman displayed distinct branches, indicating novel phylotypes. Few vaginal isolates matched food or environmental Lactobacillus species. Conclusions: Most women worldwide were colonized by three common Lactobacillus species: L. crispatus, L. jensenii and L. gasseri. Significance and Impact of Study: Knowledge of vaginal Lactobacillus species richness and distribution in women worldwide may lead to the design of better probiotic products as bacterial replacement therapy.

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Section: Genomic Dna Extractionmentioning

confidence: 99%

Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences

et al. 2002

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“…Plsamid DNA was isolated from E. coli strains by a modification of the method of Birnboim and Doly [15,16]. DNA was isolated from L. casei strains by an adaptation of the method of Currier and Nester [17,18]. PL-1 phage DNA was isolated from hightitre lysates as previously described [14].…”

Section: Dna Isolation and Characterizationmentioning

confidence: 99%

Transformation of Lactobacillus casei by electroporation

Chassy

1987

FEMS Microbiology Letters

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“…The presence of plasmids within the species of Lactobacillus has been reported since they were first discovered in this genus (Chassy et al 1976). Phenotypes such as bacteriocin production and immunity (Muriana & Klaenhammer 1987;Schillinger & Liicke 1989), lactose metabolism (Chassy et al 1978) and drug resistance (Ishiwa & Iwata 1980; Vescovo et al. 1982; Morelli et al.…”

Section: Introductionmentioning

confidence: 99%