Evidence for exposure of fish to oil spilled into the Columbia river

Krahn, Margaret M.; Kittle, Lewey J.; MacLeod, William D.

doi:10.1016/0141-1136(86)90054-1

Cited by 52 publications

(23 citation statements)

References 8 publications

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“…The metabolites in Wsh bile can provide additional information about the possible sources of PAH contamination. Phenanthrenes are released to the atmosphere during the combustion of fossil fuels, particularly coal, oil and its reWned products (O'Malley, Abrajano, & Hellou, 1996;Varanasi, Stein, & Nishimoto, 1989), naphthalene and its derivates are the main contamination due to petroleum fuel (Krahn, Kittle, & MacLeod, 1986), pyrene a widespread and common PAH is generated by many pyrolytic and petrogenic industrial processes (Ariese et al, 1993;Varanasi et al, 1989;Zhou et al, 1998), and benzo[a]pyrene is produced by many industrial processes including oil reWneries (Lin et al, 1994) often located near estuaries. Depending on the chemical structure and level of exposure, PAHs and their metabolites are putative toxic products that lead to mutagenic and/or carcinogenic eVects in Wsh and other vertebrates, including humans (MacRae & Hall, 1998;Monteiro, Reis-Henriques, & Coimbra, 2000).…”

Section: Discussionmentioning

confidence: 98%

The effect of long-term depuration on phase I and phase II biotransformation in mullets (Mugil cephalus) chronically exposed to pollutants in River Douro Estuary, Portugal

Ferreira

Moradas‐Ferreira

Reis-Henriques

2006

Marine Environmental Research

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Section: Discussionmentioning

confidence: 98%

The effect of long-term depuration on phase I and phase II biotransformation in mullets (Mugil cephalus) chronically exposed to pollutants in River Douro Estuary, Portugal

Ferreira

Moradas‐Ferreira

Reis-Henriques

2006

Marine Environmental Research

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“…A strong correlation between fish exposure to PAHs and biliary FACs has been demonstrated in previous studies (Krahn et al, 1986;Collier and Varanasi, 1991;Beyer et al, 1996Beyer et al, , 1997. Such studies used a HPLC method with fluorescence detection (HPLC/F) that was developed by Krahn et al (1980) as a rapid screening method for estimating relative amount of biliary PAH metabolites in fish without complete separation or identification of individual metabolites.…”

Section: Introductionmentioning

confidence: 87%

Evaluation of the Gill Filament-Based EROD Assay in African Sharptooth Catfish (Clarias gariepinus) as a Monitoring Tool for Waterborne PAH-Type Contaminants

et al. 2005

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The ability of African sharptooth catfish (Clarias gariepinus) in inducing cytochrome P-450 class 1A (CYP1A) and glutathione S-transferase (GST) biomarkers was determined in liver and gill filaments after 4 days of waterborne exposure to the polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P). Male (n=6) and female (n=6) fish were exposed to B[a]P added to the water (30 microg/l) corresponding to 5 mg/kg total body weight. Five female and six male fish, exposed to acetone alone added to the water served as controls. The 7-ethoxyresurufin-O-deethylase (EROD) activity was measured in hepatic microsomes and gill filaments. In addition, GST activity was determined in the hepatic cytosolic fraction and fluorescent aromatic compounds (FACs) in bile and biliverdin. Benzo[a]pyrene strongly induced EROD activities in gill filaments of both sex. Levels of FACs per ml of bile were 17-fold higher in exposed fish compared to the controls. Correlations between induction of EROD activities in gill filaments and liver and between induction of EROD activities in gill filaments and levels of biliary FACs metabolites were strong. GST activities in the hepatic cytosolic fraction were similar amongst the treated and control groups. This is the first report on studies determining EROD activities in gill filaments and hepatic tissue, FACs in bile and GST in hepatic tissues of C. gariepinus after waterborne exposure to B[a]P. The findings suggest that the gill filament-based EROD assay in C. gariepinus can be used to monitor the pollution of AhR agonists in aquatic ecosystems in eastern and southern African countries.

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“…Investigating the sources of contamination at these sites was outside the scope of this study, however, exposure reference values along with effects data have been used to determine whether biological impairments are caused or not caused by PAH contamination in Ohio streams (Cormier et al, 2000a;Cormier et al, 2002;Norton et al, 2002). Another interesting point is that exceedance of the reference values of BAP and NAPH did not always co-occur (Table 2) suggesting that it may be possible to discriminate exposures to petroleum using NAPH-type metabolites as an indicator (Krahn et al, 1984(Krahn et al, , 1986b) from exposure to combustion by-product using BAP-type metabolites (Krahn et al 1986a).…”

Section: Discussionmentioning

confidence: 98%

Reference Values for Exposure to PAH Contaminants: Comparison of Fish from Ohio and Mid-Atlantic Streams

Cormier

Lin

2005

Ecotoxicology

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Reference values for background exposures of benzo[a]pyrene (BAP)- and naphthalene (NAPH)-type metabolites were calculated for white sucker, northern hog sucker, and rock bass from the mid-Atlantic region based on the 90th percentile of a probability-based sample. Preliminary findings are presented for common carp. Bile was collected from fish and assayed for polycyclic aromatic hydrocarbon metabolites using the fluorescent method of Lin et al. Exposure reference values for white sucker were 0.3 microg/mg protein for BAP-type metabolites and 40 microg/mg protein for NAPH-type metabolites. Values from the Mid-Atlantic region were similar to those previously reported for the Eastern Corn Belt Plains (ECBP) ecoregion. Exposure reference values of BAP-type metabolites for rock bass were 0.3 microg/mg protein and 0.4 microg/mg protein for northern hog sucker. Exposure reference values of NAPH-type metabolites for rock bass were 30 microg/mg protein for rock bass and 50 microg/mg protein for northern hog sucker. Provisional values for common carp based on 39 observations are 0.4 microg/mg protein for BAP-type metabolites and 120 microg/mg protein for NAPH-type metabolites. Small streams exhibited the greatest range of exposures, 0.015-0.689 microg/mg protein for BAP-type metabolites and from 5.7 to 159 microg/mg protein for NAPH-type metabolites. Larger streams had 0.11-0.859 microg/mg protein for BAP-type metabolites and 10.2-286.5 microg/mg protein for NAPH-type metabolites. Exposure reference values for BAP and NAPH-type metabolites could be used as a basis of comparison of exposure to PAH contamination.

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Evidence for exposure of fish to oil spilled into the Columbia river

Cited by 52 publications

References 8 publications

The effect of long-term depuration on phase I and phase II biotransformation in mullets (Mugil cephalus) chronically exposed to pollutants in River Douro Estuary, Portugal

The effect of long-term depuration on phase I and phase II biotransformation in mullets (Mugil cephalus) chronically exposed to pollutants in River Douro Estuary, Portugal

Evaluation of the Gill Filament-Based EROD Assay in African Sharptooth Catfish (Clarias gariepinus) as a Monitoring Tool for Waterborne PAH-Type Contaminants

Reference Values for Exposure to PAH Contaminants: Comparison of Fish from Ohio and Mid-Atlantic Streams

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