Evidence for estrogen receptor α and β expression in skeletal muscle of pigs

Kalbe, Claudia; Mau, Marcus; Wollenhaupt, K; Rehfeldt, Charlotte

doi:10.1007/s00418-006-0224-z

Cited by 47 publications

(46 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Satellite cells were enriched by using a Percoll (Sigma Chemical Co., St. Louis, MO) gradient (90, 40, 25% in phosphate-buffered saline) according to the method of Roe et al (23). To obtain a uniform stock of cells for various experiments, a pool was established from all cells collected during several preparation procedures, as described previously (21 (24) for 7 h (short-term exposure) or 26 h (long-term exposure). In each of two to four independent experiments, a total of eight wells spread over two plates were used for each concentration of E2 (0.1 nM; 1 nM; 1 M), E1 (1 nM; 1 M), and of genistein and daidzein (0.1 M; 1 M; 10 M; 100 M).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

et al. 2008

Self Cite

View full text Add to dashboard Cite

Soy-based formulas are consumed by growing numbers of infants and used as regular food supplements in livestock production. Moreover, constituent dietary phytoestrogens may compete with endogenous estrogens and affect individual growth. This study aimed to investigate the in vitro effects of isoflavones in comparison with estrogens on the proliferation of porcine satellite cells derived from neonatal muscle. After 7 h of exposure in serumfree medium, 17␤-estradiol (1 nM, 1 M), estrone (1 M), and daidzein (1, 100 M) slightly decreased whereas 100 M genistein substantially lowered DNA synthesis. Declines in DNA amount were observed with genistein (1, 100 M) and daidzein (100 M). After 26 h of exposure, 100 M genistein reduced DNA synthesis, whereas it was increased by 10 M genistein and 10 and 100 M daidzein. In the case of 10 M genistein and 100 M daidzein, these increases apparently resulted from the repair of damaged DNA. Genistein and daidzein (100 M) reduced protein synthesis, caused a G 2 /M phase block, and decreased DNA amount in association with higher rates of cell death partially resulting from apoptosis. Conclusively, isoflavones at concentrations of greater than 1 M act as inhibitors of porcine skeletal muscle cell proliferation.

show abstract

Section: Methodsmentioning

confidence: 99%

“…So far, the impact of isoflavones and estrogens on the growth and metabolism of porcine skeletal muscle has received very little attention. Only recently have the estrogen receptors ␣ and ␤ (ER␣, ER␤), potential targets for estrogenic and isoflavonic actions, been shown to be expressed in porcine skeletal muscle (21).…”

mentioning

confidence: 99%

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…The first demonstration that ERα and ERβ are expressed in porcine skeletal muscle was in 2007 (Kalbe et al, 2007), and it suggested that skeletal muscles are a target tissue for estrogens or estrogen-like compounds. Values for the mRNA expression of ERβ in the present study were very low and likely close to the detection limit of the assay, thereby rendering their quantification quite difficult and somewhat compromising their reliability.…”

Section: Efmentioning

confidence: 99%

Exogenous genistein in late gestation: effects on fetal development and sow and piglet performance

Farmer

Robertson

Xiao

et al. 2016

animal

View full text Add to dashboard Cite

Due to their functional similarity to estradiol, phytoestrogens could prove to be beneficial in late gestating sows. The goal of this study was to determine the impact of providing the phytoestrogen genistein during late pregnancy on the performance of sows and their litters. In total, 56 gilts were equally divided into the two following groups on day 90 of gestation: (1) controls (CTL); and (2) two daily i.m. injections of 220 mg of genistein (GEN). Treatments were carried out until farrowing. Jugular blood samples were collected from 16 gilts/treatment on days 89 and 110 of gestation, and on days 3 and 21 of lactation. Milk samples were also obtained from those sows on day 3 of lactation. A male piglet from 16 CTL and 15 GEN litters was slaughtered at 24 h postpartum and a blood sample was obtained. The liver, heart and visceral organs were weighed and the semitendinosus (ST) muscle was collected and carcass composition was determined. The treatment increased (P < 0.05) the concentrations of genistein and daidzein in the plasma of gilts on day 110 of gestation and of genistein in the plasma of piglets at 24 h postpartum. It also increased IGF1 concentrations in gilts at the end of the treatment period (P < 0.05). Genistein had no impact (P > 0.1) on weight or backfat loss of sows during lactation, milk composition or weights of piglets. The pre-weaning mortality rate of piglets was very low (<7%), yet the odds ratio comparing CTL with GEN sows indicated almost twice as many chances of pre-weaning deaths occurring in litters from CTL than GEN sows. Weights of the piglet carcasses were similar for both treatments, as well as weights of the various organs and of the ST muscle ( P > 0.1). However, carcasses from GEN litters contained more fat than those from CTL litters (9.63% v. 8.34%, P < 0.05). None of the biochemical properties of the ST muscle differed between groups (P > 0.1). In conclusion, injecting gilts with 440 mg/day of genistein in late gestation increased IGF1 concentrations in gilts and carcass fat in neonatal piglets, but had minimal effect on muscle development of piglets at birth and on the performance of lactating sows and their litters.

show abstract

“…Studies using porcine satellite cell cultures focused on the regulation of Genetic factors related to animal performance 725 IGFs due to metabolic state, the role of estrogens and effects of nutrition and dietary isoflavones Theil et al, 2006;Kalbe et al, 2007 andMau et al, 2008a and. Proliferation and differentiation of satellite cells derived from low weight pigs showed lower differentiation rates than cells from medium and heavy weight littermates (Nissen and Oksbjerg, 2009).…”

Section: Muscle Fibre Traitsmentioning

confidence: 99%

Advances in research on the prenatal development of skeletal muscle in animals in relation to the quality of muscle-based food. II – Genetic factors related to animal performance and advances in methodology

Rehfeldt

Pas

Wimmers

et al. 2011

Animal

View full text Add to dashboard Cite

Selective breeding is an effective tool to improve livestock. Several selection experiments have been conducted to study direct selection responses as well as correlated responses in traits of skeletal muscle growth and function. Moreover, comparisons of domestic with wild-type species and of extreme breeds provide information on the genetic background of the skeletal muscle phenotype. Structural muscular components that differed with increasing distance in lean growth or meat quality in mammals were found to be myofibre number, myofibre size, proportions of fibre types as well as the numbers and proportions of secondary and primary fibres. Furthermore, markers of satellite cell proliferation, metabolic enzyme activities, glycogen and fat contents, the expression of myosin heavy chain isoforms, of activated AMPKa and other proteins in skeletal muscle tissue and circulating IGF1 and IGF-binding proteins have been identified to be involved in selection responses observed in pigs, cattle and/or chicken. The use of molecular methods for selective breeding of fish has only recently been adopted in aquaculture and studies of the genetic basis of growth and flesh quality traits are scarce. Some of the molecular markers of muscle structure/metabolism in livestock have also been identified in fish, but so far no studies have linked them with selection response. Genome scans have been applied to identify genomic regions exhibiting quantitative trait loci that control traits of interest, for example, muscle structure and meat quality in pigs and growth rate in chicken. As another approach, polymorphisms in candidate genes reveal the relationship between genetic variation and target traits. Thus, in large-scale studies with pigs' associations of polymorphisms in the HMGA2, CA3, EPOR, NME1 and TTN genes with traits of carcass and meat quality were detected. Other studies revealed the significance of mutations in the IGF2 and RYR1 genes for carcass lean and muscle fibre traits in pigs. Mutations in the myostatin (MSTN) gene in fish were also examined. Advances in research of the genetic and environmental control of traits related to meat quality and growth have been made by the application of holistic 'omics' techniques that studied the whole muscle-specific genome, transcriptome and proteome in relation to muscle and meat traits, the development of new methods for muscle fibre typing and the adaptation of biophysical measures to develop parameters of muscle fibre traits as well as the application of in vitro studies. Finally, future research priorities in the field are defined.Keywords: farm animal, fish, skeletal muscle, animal performance, genetic effects ImplicationsSkeletal muscle development in different animal species has been an important topic of scientific research for many years.*Authoring on behalf of all members of the COST action 925: http://www. agrsci.dk/costaction925/index.html -E-mail: rehfeldt@fbn-dummerstorf.de 718The main aim of this review is to present recent knowledge on the genetic components of skele...

show abstract

Evidence for estrogen receptor α and β expression in skeletal muscle of pigs

Cited by 47 publications

References 55 publications

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

Exogenous genistein in late gestation: effects on fetal development and sow and piglet performance

Advances in research on the prenatal development of skeletal muscle in animals in relation to the quality of muscle-based food. II – Genetic factors related to animal performance and advances in methodology

Contact Info

Product

Resources

About