Evidence for estrogen receptor-linked calcium transport in the intestine

Arjmandi, Bahram H.; Salih, Mohammed A.; Herbert, Damon C.; Sims, Simon H.; Kalu, Dike N.

doi:10.1016/s0169-6009(08)80121-3

Cited by 114 publications

(48 citation statements)

References 31 publications

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“…Administration of 17␤-estradiol at a dose of 40 g/kg b.wt./day for 21 days significantly elevated intestinal absorption of calcium in female rats whereas serum levels of 1,25-dihydroxyvitamin D were unaltered (Arjmandi et al, 1994). These findings suggest that transluminal calcium uptake is promoted by a direct action of 17␤-estradiol on the intestinal tract with no increase in the circulating levels of 1,25-dihydroxyvitamin D (Arjmandi et al, 1993(Arjmandi et al, , 1994.…”

Section: Intestinementioning

confidence: 82%

“…Since the highest serum levels of estradiol, progesterone, prolactin, follicle-stimulating hormone, and luteinizing hormone are present during estrus (Butcher et al, 1974;Brommage et al, 1990), the greatest intestinal absorption of calcium observed during estrus may be related, either directly or indirectly, to any one of several sex hormones (Brommage et al, 1993). Intestinal mucosal cells contain estrogen receptors, and calcium uptake in duodenal cells is significantly enhanced by about 60% by 17␤-estradiol at a concentration of 10 nM (Arjmandi et al, 1993). Administration of 17␤-estradiol at a dose of 40 g/kg b.wt./day for 21 days significantly elevated intestinal absorption of calcium in female rats whereas serum levels of 1,25-dihydroxyvitamin D were unaltered (Arjmandi et al, 1994).…”

Section: Intestinementioning

confidence: 99%

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Gender Differences in the Membrane Transport of Endogenous and Exogenous Compounds

2003

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Section: Intestinementioning

confidence: 82%

Section: Intestinementioning

confidence: 99%

Gender Differences in the Membrane Transport of Endogenous and Exogenous Compounds

2003

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“…Intestinal Pi absorption through the NaPi-IIb cotransporter is regulated by various physiological effectors. Epidermal growth factor (EGF) and glucocorticoids inhibit intestinal sodium-dependent Pi (Na/Pi) absorption and NaPi-IIb gene expression (2,31), whereas 1,25(OH) 2 vitamin D 3 and dietary Pi deprivation stimulate intestinal Na/Pi absorption and NaPi-IIb gene expression (17,30).…”

mentioning

confidence: 99%

“…Estrogen is involved not only in the regulation of Ca 2ϩ absorption (3,13) but also in the maintenance of bone density (3,8) and in the modulation of 1,25(OH) 2 vitamin D 3 synthesis (4,8,20,23,27). Although 1,25(OH) 2 vitamin D 3 is known to regulate intestinal Pi absorption, there is no evidence to date demonstrating estrogen regulation via NaPi-IIb cotransporter expression.…”

mentioning

confidence: 99%

Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen

Uno

Inouye

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

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The current experiments were designed to study the effect of β-estradiol on type IIb sodium-coupled phosphate (NaPi-IIb) cotransporter gene expression. Uptake studies with intestinal brush-border membrane vesicles (BBMV) showed that estrogen treatment increased sodium-dependent phosphate absorption by ∼45% in rat intestine. Northern blot analysis indicated that NaPi-IIb mRNA expression was increased by ∼50% after estrogen treatment. Western blot analysis also detected an increase in BBMV NaPi-IIb protein expression in estrogen-treated rats. In human intestinal Caco-2 cells, NaPi-IIb mRNA abundance was increased ∼60% after estrogen treatment, and this increase could be abolished by inhibition of gene transcription. Transfection studies with human NaPi-IIb promoter reporter constructs showed that the promoter was responsive to estrogen treatment. These studies demonstrate for the first time that estrogen stimulates intestinal sodium-dependent phosphate absorption in female rats. This stimulation is associated with increased NaPi-IIb mRNA and protein expression. Thus the effect of estrogen on intestinal Pi absorption may be partially due to activation of NaPi-IIb gene transcription.

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“…These actions of steroid hormones correspond with widespread distribution in various organs of ER and PR, which are members of the steroid receptor superfamily and act as an important regulator of gene transcription and cell signaling [2,4,12,15,23]. On the other hand, the duodenum and kidney are important target tissues for steroid hormone actions involved in gene expressions and calcium homeostasis [7,9,25].…”

mentioning

confidence: 98%

Duodenal and Renal Transient Receptor Potential Vanilloid 6 Is Regulated by Sex Steroid Hormones, Estrogen and Progesterone, in Immature Rats

Jung

Kim

Yang

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Transient receptor potential vanilloid 6 (TRPV6) is a major calcium channel located in the apical and basolateral membranes of cells and distributed widely in many other organs, especially the exocrine tissues, intestine and uterus. Uterine TRPV6 is affected by sex steroid hormonal changes during the estrous cycle and pregnancy. To determine whether sex steroid hormones influence the expression of TRPV6 in other tissues, we further examined TRPV6 mRNA expression in the duodenum and kidney by reverse transcriptase and real-time PCRs and its protein levels by Western blot analysis and immunohistochemistry (IHC). Immature rats were injected with 17-estradiol (E2; 40 g/kg) and/or progesterone (P4; 4 mg/kg) for three days. Treatment with a mixture of E2 and P4 (E2/P4) increased TRPV6 expression in the duodenum, and this increase was maintained when the rats were treated with receptor antagonists of estrogen (ICI 182,780; 10 mg/kg) or progesterone (RU 486; 10 mg/kg) 30 min before E2/P4 injection. However, no difference was observed in renal TRPV6 protein levels following the treatment with sex steroid hormones and/or antagonists by Western blot analysis and IHC. These results suggest that duodenal TRPV6 expression may be induced by E2 combined with P4, whereas the renal TRPV6 level is unaffected by these steroids.KEY WORDS: duodenum, immature rats, kidney, transient receptor potential vanilloid 6 (TRPV6).J. Vet. Med. Sci. 73(6): 711-716, 2011 Calcium, the most abundant cation ion in the human body, is involved in organizing the bones and teeth and working in the extra-and intracellular fluids. As a universal intracellular messenger, calcium ion modulates neurotransmission, muscle constructions and secretion. Calcium ion enters the cell through the tight junctions between epithelial cells or through the apical and basolateral cell membranes. Calcium ion removal from epithelial cells requires apical calcium entry channels, including the intracellular calcium binding protein calbindin-D 9k , and the calcium pump plasma membrane calcium ATPase (PMCA1b) [5,10,13]. Transcellular calcium transport occurs through apical transient receptor potential vanilloid (TRPV) calcium channels [21]. The intestine, kidney and placenta are the three major organs implicated in calcium transport by calcium absorption from the diet, renal reabsorption and calcium transport from the maternal to fetal circulation, respectively [5]. In particular, maintenance of the uterine calcium balance is of crucial importance for many physiological functions, including smooth muscle contraction and embryo implantation. To obtain the calcium ion levels required for pregnancy and labor, the body uses numerous calcium transport genes, including CaBPs, PMCA and TRPVs [10,13]. These genes are regulated by various factors, i.e., vitamin D, dietary calcium ion and steroid hormones, and many reports have shown the expression of TRPV6 gene during the estrous cycle and pregnancy [3,6,[28][29][30].The actions of sex steroid hormones, i.e., estrogen (E2)...

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Evidence for estrogen receptor-linked calcium transport in the intestine

Cited by 114 publications

References 31 publications

Gender Differences in the Membrane Transport of Endogenous and Exogenous Compounds

Gender Differences in the Membrane Transport of Endogenous and Exogenous Compounds

Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen

Duodenal and Renal Transient Receptor Potential Vanilloid 6 Is Regulated by Sex Steroid Hormones, Estrogen and Progesterone, in Immature Rats

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