Evidence for erythrocyte-specific histone modification and structural changes in chromatin during goose erythrocyte maturation

Brasch, Klaus; Adams, G. H. M.; Neelin, J. M.

doi:10.1242/jcs.15.3.659

Cited by 34 publications

(1 citation statement)

References 42 publications

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“…The existence of a unique chromatin structure in Physarum polycephalum (Scheer et al, 1981) might be related to the salt solubilization behavior of H2B. Alternatively, Physarum H2B may be less tightly bound to DNA than are H2B histones from other sources since it is known that phosphorylated histones are more easily extracted from transcriptionally active cells (Urban et al, 1981) than from mature cells (Brasch et al, 1974).…”

Section: Discussionmentioning

confidence: 99%

High mobility group like chromosomal proteins from amebas of the acellular slime mold Physarum polycephalum

Côté¹,

Neelin²,

Pallotta³

1985

Biochemistry

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Section: Discussionmentioning

confidence: 99%

High mobility group like chromosomal proteins from amebas of the acellular slime mold Physarum polycephalum

Côté¹,

Neelin²,

Pallotta³

1985

Biochemistry

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Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of friend leukemia cells

Darzynkiewicz¹,

Traganos²,

et al. 1984

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Friend leukemia cells from exponentially growing or differentiated (DMSO-induced) cultures were permealized and their DNA was stained with 4'6-diamidino-2-phenylindole (DAPI), Hoechst 33342, acridine organge, ethidium bromide, propidium iodide, quinacrine, 7-amino-actinomycin D, mithramycin, or chromomycin AB. Accessibility of DNA to each of the above fluorochromes was compared in differentiated and nondifferentiated cells before and after nuclear proteins, mostly histones, were extracted with 0.1N HCl. A decrease in the accessibility of DNA to several dyes, especially pronounced in the case of some intercalators, was observed in differentiated cells. After extraction of nuclear proteins with HCl there was an increase in DNA accessibility, of varying degree depending on the fluorochrome and the difference between differentiated and nondifferentiated cells was abolished for most of the intercalating dyes. The increase was the lowest for DAPI (45%), the highest for 7-amino-actinomycin D (13-fold), and in general was higher for the intercalating dyes that unwind DNA than for dyes binding externally to the double helix. The results are discussed in terms of the mode of interactions between DNA and the fluorochromes and factors associated with chromatin structure that may affect accessibility of DNA in situ in exponentially growing and differentiated cells.

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Chromatin texture changes related to proliferation and maturation in erythrocytes

Giroud

Seigneurin

et al. 1988

Cytometry

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Erythroid cell types were quantitatively assessed on the basis of the analysis of their nuclear images after Feulgen and Heidenhain blue counterstaining. An original nonsupervised classification method, developed in our laboratory, was applied to cell cycle analysis. Results obtained suggest that, in the four proliferative generation cycles, which concur with the successive morphological stages, there is a sequential flow of cells from one morphological stage to the next by cell cycling. We show that the chromatin pattern alone made it possible to set up a metric of the whole differentiation process occurring in the normal human erythroblastic lineage in which proliferating events occur. Key terms: Cell cycle analysis, differentiation in erythroblastic lineage, erythropoiesisErythropoiesis is an appropriate model for analyzing descriptors obtainable by means of high-resolution imcell cycle kinetic events and the maturation process in age analysis may become more accurate and convenient morphological changes during the evolution from prim-for functional interpretation (36,43,44). itive precursor cell to the mature erythrocyte, Differen-In a previous paper (261, we described a quantitative tiation of erythroid cells proceeds through a series of color image analysis of the normal maturation sequence recognizable morphological changes, which give rise to of the erythroblastic May-Grunwald-Giemsa (MGG) a cell type classification used by hematologists. The stained cells using the SAMBA 200 image analyzer. We subsequent stages of erythroid maturation are termed concluded that the machine successfully reproduced the mature erythrocyte is continuous although arbitrarily "he structural changes occurring during differentia-broken up by hematologists. tion are associated with striking biochemical alterationsThe present investigation deals with a combined quanas shown by combined methods of morphology and au-titative study of cell proliferation and cell differentiation toradiography (10,16,18,21,30,33,35), Unfortunately, of Feulgen Heidenhaim CFH1 blue-stained erythroid cells, these methods are time-consuming, and discrepancies in based on the concept that the functional properties of a the results reported were probably due to errors in the cell (DNA synthesis and template DNA activity) are experimental techniques used (34). A new approach to reflected in chromatin pattern. The nonsupervised this problem has been provided by analytical cytology method of classification (3,4) has the advantage of permethods based on computer-assisted cell-image process-mitting cell kinetic analysis from a single sample of a ing. The results already obtained by Zajicek et al. (46,471 cell population and does not require any a priori knowland Dormer et al. (17,19,20) for the characterization of edge about the proliferating state of the cells or a kinetic the grey-level images of erythroblasts at the various model of cell population. The results obtained will be steps of the maturation process have shown that it is discussed in terms of the coo...

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Evidence for erythrocyte-specific histone modification and structural changes in chromatin during goose erythrocyte maturation

Cited by 34 publications

References 42 publications

High mobility group like chromosomal proteins from amebas of the acellular slime mold Physarum polycephalum

High mobility group like chromosomal proteins from amebas of the acellular slime mold Physarum polycephalum

Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of friend leukemia cells

Chromatin texture changes related to proliferation and maturation in erythrocytes

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