Evidence for Enzyme-bound β-Aspartyl Phosphate in the Aspartokinase Reaction

Threonine-sensitive aspartokinase-homoserine dehydrogenase of Escherichia coli K 12. Reaction with 6-mercapto-9-β-D-ribofuranosylpurine 5'-triphosphate

“…At the end of the experiments, the mixtures were lyophilized, and the powders were resuspended in 0.5 ml of water. A 10-µ1 sample from each mixture was submitted to paper electrophoresis according to Manders et al (1969).…”