Evidence for Enhanced Telomerase Activity in Barrett's Esophagus with Dysplasia and Adenocarcinoma

Merchant, Nipun B.; Dutta, Sudhir K.; Girotra, Mohit; Arora, Manish; Meltzer, Stephen J.

doi:10.7314/apjcp.2013.14.2.679

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…Souza et al directly measured telomere lengths in biopsy specimens from individuals with vs without GERD, and found shorter telomeres in patients with GERD, although TERT levels seemed to be similar between groups 73 . In addition, TERT activity is significantly higher in esophageal tissues from patients with BE with dysplasia or BE with EAC than from patients with BE without dysplasia 74 .…”

Section: Lncrnas In Be and Eacmentioning

confidence: 90%

Long Noncoding RNAs in the Pathogenesis of Barrett’s Esophagus and Esophageal Carcinoma

Abraham

Meltzer

2017

Gastroenterology

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For many years, only a small fraction of the human genome was believed to regulate cell function and development. This protein-coding portion composed only 1% to 2% of 3 billion human DNA base pairs— the remaining sequence was classified as junk DNA. Subsequent research has revealed that most of the genome is transcribed into a broad array of noncoding RNAs, ranging in size from microRNA (20–23 nucleotides) to long noncoding RNA (lncRNA, more than 200 nucleotides). These noncoding RNA classes have been shown to use diverse molecular mechanisms to control gene expression and organ system development. As anticipated, alterations in this large control system can contribute to disease pathogenesis and carcinogenesis. We review the involvement of noncoding RNAs—lncRNAs in particular—in development of Barrett's esophagus and esophageal carcinoma.

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Section: Lncrnas In Be and Eacmentioning

confidence: 90%

Long Noncoding RNAs in the Pathogenesis of Barrett’s Esophagus and Esophageal Carcinoma

Abraham

Meltzer

2017

Gastroenterology

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“…The detection of increased telomerase activity and shortening of telomeres are hypothesized to serve as specific signals of carcinogenesis. According to a number of studies (25)(26)(27), the detection of telomerase activity or telomere length alterations may be observed either in migrating cancer cells or in peripheral blood leukocytes. Such results may provide an opportunity to distinguish or qualify patients with differential cancer stages or grades.…”

Section: Discussionmentioning

confidence: 99%

Telomere length assessment in leukocytes presents potential diagnostic value in patients with breast cancer

et al. 2016

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Abstract.Telomere shortening is associated with cancer development, primarily through the induction of genomic instability. The majority of studies have indicated that individuals with shorter blood telomeres may be at a higher risk of developing various types of cancer. There is increasing evidence that the study of the alterations in telomere length may improve cancer prognosis. The aim of the present study was to verify the use of telomere length parameters in the diagnostics of breast cancer stage. Telomere length was analyzed in the blood leukocytes of 52 patients with breast cancer relative to 47 control subjects using quantitative polymerase chain reaction. The effects of stage, grade, estrogen receptor, progesterone receptor and human epidermal growth factor 2 (HER2) status were assessed. The current study demonstrated that the average telomeric sequence length was significantly shorter in leukocytes from individuals diagnosed with a more severe stage of breast cancer (T2N1M0) than in leukocytes in the early stages of the disease (T1N0M0) (P=0.0207). Furthermore, the data indicated that telomeres in leukocytes derived from patients with HER2 + breast cancer were significantly longer compared with those with the HER2 -type (P=0.0347). These results suggest that the assessment of telomeres in blood leukocytes may, at least partially, correspond with breast cancer staging and HER2 receptor status.

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“…Telomerase activity was measured by modified ТRAP method (Fig. 2 ) as described earlier [ 15 , 16 ]. Telomerase is an enzyme necessary for the maintenance of chromosomes stability in the course of cell division that most likely plays a key role in maintaining cellular proliferative potential and tumor growth [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Implication of Gastric Cancer Molecular Genetic Markers in Surgical Practice

Nemtsova

Стрельников

Танас

et al. 2017

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Introduction: We have investigated aberrant methylation of genes CDH1, RASSF1A, MLH1, N33, DAPK, expression of genes hTERT, MMP7, MMP9, BIRC5 (survivin), PTGS2, and activity of telomerase of 106 gastric tumor samples obtained intra-operatively and 53 gastric tumor samples from the same group of patients obtained endoscopically before surgery. Biopsy specimens obtained from 50 patients with chronic calculous cholecystitis were used as a control group. Together with tissue samples obtained from different sites remote to tumors, a total of 727 samples have been studied. The selected parameters comprise a system of molecular markers that can be used in both diagnostics of gastric cancer and in dynamic monitoring of patients after surgery. Special attention was paid to the use of molecular markers for the diagnostics of malignant process in the material obtained endoscopically since the efficacy of morphological diagnostics in biopsies is compromised by intratumoral heterogeneity, which may prevent reliable identification of tumor cells in the sampling. Our data indicated that certain molecular genetic events provided more sensitive yet specific markers of the tumor. Conclusion: We demonstrated that molecular profiles detected in preoperative biopsies were confirmed by the material obtained intra-operatively. The use of endoscopic material facilitates gastric tumors pre-operative diagnostics, improving early detection of gastric cancer and potential effective treatment strategies.

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Evidence for Enhanced Telomerase Activity in Barrett's Esophagus with Dysplasia and Adenocarcinoma

Cited by 3 publications

References 30 publications

Long Noncoding RNAs in the Pathogenesis of Barrett’s Esophagus and Esophageal Carcinoma

Long Noncoding RNAs in the Pathogenesis of Barrett’s Esophagus and Esophageal Carcinoma

Telomere length assessment in leukocytes presents potential diagnostic value in patients with breast cancer

Implication of Gastric Cancer Molecular Genetic Markers in Surgical Practice

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