“…Qualitative and quantitative analyses were conducted on the EOM by capillary gas chromatography coupled to mass spectrometry (GC-MS) to identify organic molecules, particularly biomarkers (Faure et al, 2000;Jeanneau et al, 2008a;2008b;Biache et al, 2011;Bertrand et al, 2012). For the qualitative series, dry extracts (500 µL of EOM) were solubilized in 250 µL of DCM and 250 µL of BSTFA (N, O bis(trimethylsilyl)trifluoroacetamide) and heated at 60°C during 15 min to activate the silylation, before being injected into a GC (Agilent Technology 6890 N) coupled to a MS (Agilent Technology 5973 Inert Mass Selective Detector).…”