Evidence for crossing the blood barrier of adult rat brain by human adipose-derived mesenchymal stromal cells during a 6-month period of post-transplantation

Haddad-Mashadrizeh, Aliakbar; Bahrami, Ahmad Reza; Matin, Maryam Moghaddam; Edalatmanesh, Mohammad Amin; Zomorodipour, Alireza; Fallah, Ali; Gardaneh, Mossa; Kia, Naghmeh Ahmadian; Sanjarmoosavi, Naser

doi:10.1016/j.jcyt.2013.03.003

Cited by 16 publications

(12 citation statements)

References 47 publications

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“…The microvascular density in the ALLG group was greater compared with the AFT group (n = 6). Scale bar 200 lm eliciting an immune response [16][17][18], while other studies have shown opposite results [19][20][21]. As few studies have reported allogeneic ASCs-assisted fat grafting in animal models with immunocompetence, our aim was to investigate whether allogeneic ASCs can facilitate the survival of fat grafts.…”

Section: Discussionmentioning

confidence: 99%

“…Although some studies indicated that allogeneic mesenchymal stem cells (MSCs) can elicit donor-specific immune responses in immunocompetent animals [31,32], many studies in vitro and in vivo showed that allogeneic MSCs exerted low immunogenicity and immunosuppression [16,17]. So far, it is unclear whether allogeneic MSCs have immune privilege and promote fat survival in fat grafting.…”

Section: Discussionmentioning

confidence: 99%

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Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats

et al. 2015

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“…An inbred strain, such as Wistar rat, may further minimize the potential immunologic impact on results. However, low immunogenicity was a typical property of MSCs, which has been demonstrated by several studies previously [48–50]. This was also one of the important reasons that MSCs were considered the promising seeding cells in regenerative medicine.…”

Section: Discussionmentioning

confidence: 92%

Combination of mesenchymal stem cell injection with icariin for the treatment of diabetes-associated erectile dysfunction

Wang

Liu

et al. 2017

PLoS ONE

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The present study was aimed to examine whether icariin, a traditional Chinese medicine, could improve therapeutic effects of adipose derived mesenchymal stem cells (ADSCs) for diabetes-associated erectile dysfunction (DMED). DMED were induced in rats by intraperitoneal injection of streptozotocin and confirmed by erectile function measurement. Then, rats of diabetic ED were randomly divided to receive the treatment of saline, ADSCs, icariin or ADSCs combined with icariin respectively. Compared with the treatment by ADSCs or icariin alone, intracavernosum injection of ADSCs combined with the following daily gastric gavage of icariin significantly augmented the value of ICP and ICP/MAP (p<0.01). Meanwhile, the survival of transplanted ADSCs was much improved due to the application of icariin. Similarly, immunofluorescent staining analysis demonstrated that the improved erectile tissue structure by combination of ADSCs and icariin was significantly associated with the increased expression of endothelial markers (vWF) (p<0.01) and smooth muscle markers (α-SMA) (p<0.01). Furthermore, the structure changes in corpus cavernosum were further confirmed by the Masson’s trichrome staining. To explore the possible mechanism underlying icariin-enhanced therapeutic efficacy of MSCs, we employed an in vitro testing system by introducing H2O2 to imitate oxidative stress condition considering the oxidative environment faced by engrafted ADSCs and anti-oxidative capacity of icariin. In vitro, we found that the addition of icariin considerably reduced the apoptosis of ADSCs, and attenuated the intracellular reactive oxygen species (ROS), the superoxidase dismutase (SOD) activity and the lactate dehydrogenase (LDH). Subsequently, we examined the expression of apoptosis-related proteins and explored the potential signaling pathway through which icariin promoted the survival of ADSCs against oxidative stress. It was demonstrated that icariin significantly inhibited the upregulation of apoptosis-related proteins under oxidative condition, including Bax and cleaved caspase-3, while promoted the expression of anti-apoptotic factor BCL2. These effects were accompanied with the activation of signal molecules, PI3K/Akt and STAT3. The further signal protein inhibition assays exhibited that the suppression of STAT3 abrogated the icariin-mediated anti-apoptotic effects observed above, while did not influence the expression of PI3K/Akt. However, PI3K inhibition could abrogate icariin–mediated STAT3 activation and achieved a similar effect as STAT3 inhibition. Our results suggested that icariin was an effective adjuvant for enhancing ADSC-based therapy of DMEM, which may be ascribed to their protection of ADSCs against oxidative stress via the regulation of PI3K/Akt-STAT3 signal pathway.

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“…Fifteen days after transplantation, the rats were anesthetized (ketamine 50 mg/mL and xylazine 5 mg/mL, i.p. ), perfused with 4% buffered paraformaldehyde (Haddad‐Mashadrizeh et al, ) and the remaining implanted scaffolds were individually dissected and removed from the dorsum of rats. The tissues from lung and spleen were also removed.…”

Section: Methodsmentioning

confidence: 99%

Injectable hydrogel delivery plus preconditioning of mesenchymal stem cells: exploitation of SDF‐1/CXCR4 axis toward enhancing the efficacy of stem cells' homing

Naderi‐Meshkin

Matin

Heirani‐Tabasi

et al. 2016

Cell Biology International

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Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target injury tissues for the long term. However, their homing efficiency has been observed to be very poor because of the lack or modifications of homing factors SDF-1α and CXCR4 receptors. Hence, this study was designed to investigate the homing and retention of pretreated human adipose tissue-derived MSCs (hASCs) from three different delivery routes in response to SDF-1α, released from chitosan-based injectable hydrogels. After stimulation of ASCs with a hypoxia mimicking agent, the expression level and functionality of CXCR4 were analyzed by flowcytometric analysis (FACS), transwell migration assay and qPCR. Then, the homing/retention of pretreated DiI-labeled hASCs were compared through three different in vivo delivery routes, 2 weeks after transplantation in Wistar rats. The cells were tracked histologically by fluorescent microscope and by PCR for human-specific CXCR4 gene. Results showed CXCR4 has dynamic expression pattern and pretreatment of hASCs significantly up-regulates CXCR4, leading to an increase in migration capacity toward 100 ng/mL SDF-1α in vitro and homing into the subcutaneously implanted hydrogel releasing SDF-1α in vivo. Furthermore, it seems that SDF-1α is particularly important in the retention of ASCs, in addition to its chemoattraction role. In summary, the delivery route in which the ASCs were mixed with the hydrogel rather than systemic delivery and local injection and preconditioning undertaken to increase CXCR4 expression concomitant with SDF-1α delivery by the injectable hydrogel, allowed for further homing/retention of ASCs. This might be a promising way to get better therapeutic outcomes in stem cell therapy.

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Evidence for crossing the blood barrier of adult rat brain by human adipose-derived mesenchymal stromal cells during a 6-month period of post-transplantation

Cited by 16 publications

References 47 publications

Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats

Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats

Combination of mesenchymal stem cell injection with icariin for the treatment of diabetes-associated erectile dysfunction

Injectable hydrogel delivery plus preconditioning of mesenchymal stem cells: exploitation of SDF‐1/CXCR4 axis toward enhancing the efficacy of stem cells' homing

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