Evidence for coexistence between calcitonin gene-related peptide and serotonin in the bulbospinal pathway in the monkey

Arvidsson, Ulf; Schalling, Martin; Culheim, Staffan; Ulfhake, Brun; Terenius, Lars; Verhofstad, A.; Hökfelt, Tomas

doi:10.1016/0006-8993(90)91740-8

Cited by 33 publications

(11 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, just as in the rat (CortCs et al, 1990a;Abelson and Mycevich, 1991), a population of monkey spinal motoneurons express CCK mRNA. Also, CGRP mRNA could be visualized in motoneurons in adjacent sections, confirming the presence of this peptide in primate motoneurons (Gibson et al, 1984(Gibson et al, , 1988aArvidsson et al, 1990Arvidsson et al, , 1991. The lack of CGRP mRNA in the primate dorsal horn confirms earlier immunohistochemical studies strongly suggesting that virtually all CGRP in the dorsal horn originates in the dorsal root ganglia (Carlton et al, 1987(Carlton et al, , 1988, as is the case in many other species (Ishida-Yamamoto and Tohyama, 1989).…”

Section: Cck In Motoneuronssupporting

confidence: 82%

Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

Verge

Wiesenfeld‐Hallin

Hökfelt

1993

Eur J of Neuroscience

100

View full text Add to dashboard Cite

The peptide cholecystokinin (CCK) has been suggested to be involved in nociception, but its exact localization at the level of the spinal cord and in spinal ganglia has been a controversial issue. Therefore the distribution of messenger RNA (mRNA) for CCK was studied by in situ hybridization using oligonucleotide probes on sections of adult rat lumbar dorsal root ganglia following unilateral section of the sciatic nerve and on sections of untreated monkey trigeminal ganglia, spinal cord and spinal ganglia from all levels. For comparison, calcitonin gene-related peptide (CGRP) mRNA was also studied in the monkey tissue using the same techniques. Peripheral sectioning of the sciatic nerve in the rat resulted in the appearance of detectable CCK mRNA in up to 30% of remaining ipsilateral L4 and L5 dorsal root ganglion neurons 3 weeks after surgery, with a distinct but more limited appearance also in the contralateral ganglia. No cells, or only single cells, could be seen in normal control rat ganglia. In contrast, in the normal monkey, approximately 20% of dorsal root ganglion neurons, regardless of spinal level, and 10% of trigeminal ganglia neurons expressed mRNA for CCK. CGRP mRNA was expressed at detectable levels in approximately 80% of these monkey dorsal root ganglion neurons. In the monkey spinal cord, CCK mRNA was detected in the dorsal horn and in motoneurons, whereas CGRP mRNA was only seen in motoneurons. The present results suggest that CCK peptides can be involved in sensory processing in the dorsal horn of the spinal cord in normal monkeys and in rats after peripheral nerve injury, adding one more possible excitatory peptide to the group of mediators in the dorsal horn.

show abstract

Section: Cck In Motoneuronssupporting

confidence: 82%

Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

Verge

Wiesenfeld‐Hallin

Hökfelt

1993

Eur J of Neuroscience

100

View full text Add to dashboard Cite

show abstract

“…In addition, there was a strong serotoninergic innervation of the mouse somatic motor nuclei throughout the spinal cord, similar to that described in the cat (Arvidsson et al, 1990b), primate (Arvidsson et al, 1990a), and rat (Jones and Light, 1990;Marlier et al, 1991). These 5HT-IR axons mainly originate from RPa/ROb neurons and modulate the excitability of somatic motoneurons (Hounsgaard et al, 1988).…”

Section: Serotoninergic Fibersmentioning

confidence: 61%

The organization of the brainstem and spinal cord of the mouse: Relationships between monoaminergic, cholinergic, and spinal projection systems

VanderHorst

Ulfhake

2006

Journal of Chemical Neuroanatomy

Self Cite

110

101

View full text Add to dashboard Cite

“…In 5-HT neurons at the caudal level of rats and monkeys, it has been reported that several peptides, such as SP, TRH, ENK, and CGRP, coexist with 5-HT [28][29][30][31][32]. Whether these peptides are expressed in the caudal 5-HT neurons of mouse brain remains unclear.…”

Section: Resultsmentioning

confidence: 98%

“…In 5-HT neurons at the rostral level, the expressions of several peptides, including GAL, CRF, and SP, have been reported in rat and human DRN neurons, whereas no coexistence of these peptides with 5-HT in mouse DRN has been detected [17,[25][26][27]. In rat and monkey, peptides SP, TRH, ENK, and CGRP have been reported to coexist with 5-HT in 5-HT neurons at the caudal level [28][29][30][31][32]. In this study, we showed that these peptides also co-localized with 5-HT in developing mouse brain at the caudal level (Fig.…”

Section: Discussionmentioning

confidence: 99%

<italic>Lmx1b</italic> controls peptide phenotypes in serotonergic and dopaminergic neurons

Yan¹,

Huang²,

Xie³

et al. 2013

ABBS

View full text Add to dashboard Cite

Serotonin (5-HT) neurons synthesize a variety of peptides. How these peptides are controlled during development remains unclear. It has been reported that the co-localization of peptides and 5-HT varies by species. In contrast to the situations in the rostral 5-HT neurons of human and rat brains, several peptides do not coexist with 5-HT in the rostral 5-HT neurons of mouse brain. In this study, we found that the peptide substance P and peptide genes, including those encoding peptides thyrotropin-releasing hormone, enkephalin, and calcitonin gene-related peptide, were expressed in the caudal 5-HT neurons of mouse brain; these findings are in line with observations in rat and monkey 5-HT neurons. We also revealed that these peptides/peptide genes partially overlapped with the transcription factor Lmx1b that specifies the 5-HT cell fate. Furthermore, we found that the peptide cholecystokinin was expressed in developing dopaminergic neurons and greatly overlapped with Lmx1b that specifies the dopaminergic cell fate. By examining the phenotype of Lmx1b deletion mice, we found that Lmx1b was required for the expression of above peptides expressed in 5-HT or dopaminergic neurons. Together, our results indicate that Lmx1b, a key transcription factor for the specification of 5-HT and dopaminergic transmitter phenotypes during embryogenesis, determines some peptide phenotypes in these neurons as well.

show abstract

Evidence for coexistence between calcitonin gene-related peptide and serotonin in the bulbospinal pathway in the monkey

Cited by 33 publications

References 59 publications

Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

The organization of the brainstem and spinal cord of the mouse: Relationships between monoaminergic, cholinergic, and spinal projection systems

<italic>Lmx1b</italic> controls peptide phenotypes in serotonergic and dopaminergic neurons

Contact Info

Product

Resources

About

Evidence for coexistence between calcitonin gene-related peptide and serotonin in the bulbospinal pathway in the monkey

Cited by 33 publications

References 59 publications

Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

Cholecystokinin in Mammalian Primary Sensory Neurons and Spinal Cord: In Situ Hybridization Studies in Rat and Monkey

The organization of the brainstem and spinal cord of the mouse: Relationships between monoaminergic, cholinergic, and spinal projection systems

&lt;italic&gt;Lmx1b&lt;/italic&gt; controls peptide phenotypes in serotonergic and dopaminergic neurons

Contact Info

Product

Resources

About

<italic>Lmx1b</italic> controls peptide phenotypes in serotonergic and dopaminergic neurons