Evidence for an Unconventional Radiosensitivity of Rat 9L Subcutaneous Tumors

Wallen, C. Anne; Michaelson, Sol M.; Wheeler, Kenneth T.

doi:10.2307/3575491

Cited by 60 publications

(33 citation statements)

References 22 publications

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“…The 9L glioma has been used extensively as a model for studies of radiotherapy (Leith et al, 1975;Wallen et al, 1980). Our data support the resistant nature of the 9L glioma shown by previous investigators (Leith et al, 1975;Wallen et al, 1980).…”

Section: Discussionsupporting

confidence: 82%

“…Recently, this tumour has been described as a tissue isolate grown on the epigastric branch of the femoral vessels (Evans and Koch, 1994). Typically, intracerebral and small subcutaneous 9L tumours are characterised as having minimal necrosis and no severe hypoxia (Leith et al, 1975;Wallen et al, 1980). However the 9L glioma has also been reported to contain uniform or moderate, intermittent hypoxia (Moulder and Rockwell, 1984;Wong et al, 1990;Franko et al, 1992).…”

mentioning

confidence: 99%

“…After successive in vivo and in vitro transfers, a cell line was established (9L) which produced a gliosarcoma when implanted intracerebrally. Since that time, the 9L has been used extensively as a subcutaneous and intracerebral tumour model, especially for studies of radiosensitivity (Leith et al, 1975;Wallen et al, 1980). Recently, this tumour has been described as a tissue isolate grown on the epigastric branch of the femoral vessels (Evans and Koch, 1994).…”

mentioning

confidence: 99%

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Identification of hypoxia in cells and tissues of epigastric 9L rat glioma using EF5 [2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide]

Evans

Joiner²,

Jenkins³

et al. 1995

Br J Cancer

103

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Section: Discussionsupporting

confidence: 82%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of hypoxia in cells and tissues of epigastric 9L rat glioma using EF5 [2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide]

Evans

Joiner²,

Jenkins³

et al. 1995

Br J Cancer

103

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“…However, when some specific tumours were compared at different sizes, there was a trend for the hypoxic fraction to increase as size increased, although this was not true for all tumour types (Stanley et al, 1977;Siemann, 1980;Wallen et al, 1980;Okunieff et al, 1986;Fu et al, 1990). Over the size range of 4-to 12-mm diameter, SaF, CaNT and Rh tumours showed no correlation between size and hypoxic fraction measured by flow cytometric analysis of bioreductively bound NITP abducts (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Cell cycle distribution of hypoxia and progression of hypoxic tumour cells in vivo

Webster

Hodgkiss²,

Wilson³

1998

Br J Cancer

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Summary Hypoxia was assessed in three murine tumour models in vivo by measuring the incorporation of 7-(4'-(2-nitroimidazole-1-yl)-butyl)-theophylline (NITP), an immunologically identifiable hypoxia marker that binds bioreductively to cells under low-oxygen conditions. Proliferating cells were labelled in the same tumours by administering the thymidine analogue bromodeoxyuridine (BrdUrd). The relative hypoxia in each cell cycle phase of cells isolated from tumours was assessed by addition of propidium iodide with analysis by flow cytometry. There was no relationship between tumour volume and hypoxia in either the anaplastic sarcoma SaF or the poorly differentiated carcinoma CaNT and only a slight negative correlation in moderately well-differentiated carcinoma Rh. The G1/Go phase contained the greatest number of aneuploid hypoxic cells (aneuploid hypoxia ranging from less than 1% up to 40%, 38% and 71% in SaF, CaNT and Rh respectively), although there were significant amounts of hypoxia present in S-and G/M phases for all three tumours examined. However, the highest proportion of hypoxia occurred in the G/M phase, in which up to 60% of the cells were hypoxic. Simultaneous measurement of hypoxia, proliferation and DNA content using a novel triple-staining flow cytometry method showed that hypoxic cells could actively participate in the cell cycle. In addition, the cell cycle distribution of NITP and BrdUrd labelling showed that hypoxic cells could progress through the cell cycle, although their rate of progression was slower than that of better oxygenated cells.

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“…(Barker et al, 1973;Wheeler et al, 1979) and s.c. tumours, and the in situ growth characteristics CCNU (4,8,12,16,or 20mgkg-1) either alone or simultaneously with 2.5 mmol kg-1 (0.5mg kg-1) of MISO. After 20-24 h, the rats were killed by cervical dislocation, the tumours removed and assayed for colony formation as previously described (Rosenblum et al, 1975a;Wallen et al, 1980). Tumours from rats injected with saline, MISO alone or HPMC were handled similarly and assayed in each experiment.…”

Section: In Situ Tumour Systemsmentioning

confidence: 99%