Evidence for an intra-tumoral microbiome in pituitary neuroendocrine tumors with different clinical phenotypes

Ye, Lei; Wu, Bingshan; Xu, Ziao; Ji, Xuefei; Guan, Liao; Li, Pingping; Li, Yan; Cheng, Hongwei; Xiao, Jin

doi:10.1007/s11060-023-04318-2

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…This may be attributable to the differences in the composition of the microbiome between the oral cavity and the gastrointestinal tract. Additionally, we recently performed a case-only analysis of intratumoral microbes in different phenotypes of PA ( 30 ). However, we did not find an overlap between the salivary microbes and intratumoral microbes.…”

Section: Discussionmentioning

confidence: 99%

Salivary microbiome profiles for different clinical phenotypes of pituitary adenomas by single-molecular long-read sequencing

Ji,

Li,

Guo

et al. 2023

Microbiol Spectr

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Pituitary adenomas (PAs) are common benign brain tumors. Although associations between the gastrointestinal microbiome and PA have been reported previously, studies on the distributions of salivary microbial species in PA patients and among their different clinical phenotypes are currently lacking. In this study, saliva samples from 42 patients and 20 healthy individuals were selected for third-generation sequencing. The PA group included four clinical phenotypes: adrenocorticotropic hormone-secreting PA ( n = 6), growth hormone-secreting PA ( n = 9), prolactin-secreting PA ( n = 18), and nonfunctioning PA ( n = 9). All samples were sequenced, and the data were clustered and de-chimerized to obtain information regarding the abundance of operational taxonomic units. We found that the species distributions in the saliva of PA patients were more abundant than those of healthy individuals. A total of 82 genera were identified across all samples, of which 14 and 17 genera were more abundant in the saliva samples of patients with PA and healthy individuals, respectively. In the phenotypic functional prediction, the phenotypes of anaerobic and Gram-positive organisms were more commonly seen in patients with PA than in healthy individuals. The bioinformatics prediction indicated that multiple metabolic pathways were involved in the pathogenesis of PA. In conclusion, this study highlighted the associations of salivary microbiome profiles with PA, which may improve the existing understanding of the pathogenesis of PA and provide diagnostic and therapeutic targets for PA. IMPORTANCE The gut and salivary microbiomes have been widely reported to be significantly associated with a number of neurological disorders. The stability of the microbiome in the oral cavity makes it a potentially ideal sample that can be conveniently obtained for the investigation of microbiome-based pathogenesis in diseases. In the present study, we used a single-molecule long-read sequencing technique to study the distribution of the salivary microbiota in patients with pituitary adenoma (PA) and healthy individuals, as well as among four clinical phenotypes of PA. We found that the diversity of salivary microbes was more abundant in PA patients than in healthy individuals. We also observed some unique genera in different PA phenotypes. The bioinformatics-based functional predictions identified potential links between microbes and different clinical phenotypes of PA. This study improves the existing understanding of the pathogenesis of PA and may provide diagnostic and therapeutic targets for PA.

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Section: Discussionmentioning

confidence: 99%

Salivary microbiome profiles for different clinical phenotypes of pituitary adenomas by single-molecular long-read sequencing

Ji,

Li,

Guo

et al. 2023

Microbiol Spectr

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“…As an important component of tumor microenvironment and a vital regulator of tumor biology, 116 macrophages are also regulated by the intratumor microbiome. In pituitary neuroendocrine tumors, intratumor bacteria were associated with more abundant microglia and the macrophages in nerve tissues, which further showed longitudinally branched morphology, 117 indicating the regulatory role of intratumor microbiome on macrophages. Similarly, more abundantly infiltrated M2 macrophages were detected in bacteria‐dominant subtype in HBV‐related hepatocellular carcinoma, which further showed multiple upregulated metabolism pathways 118 .…”

Section: Mechanisms Of Intratumor Microbiome In Modulating Tumor Init...mentioning

confidence: 97%

Intratumor microbiome: selective colonization in the tumor microenvironment and a vital regulator of tumor biology

Jiang,

Yang,

Dai

et al. 2023

MedComm

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The polymorphic microbiome has been proposed as a new hallmark of cancer. Intratumor microbiome has been revealed to play vital roles in regulating tumor initiation and progression, but the regulatory mechanisms have not been fully uncovered. In this review, we illustrated that similar to other components in the tumor microenvironment, the reside and composition of intratumor microbiome are regulated by tumor cells and the surrounding microenvironment. The intratumor hypoxic, immune suppressive, and highly permeable microenvironment may select certain microbiomes, and tumor cells may directly interact with microbiome via molecular binding or secretions. Conversely, the intratumor microbiomes plays vital roles in regulating tumor initiation and progression via regulating the mutational landscape, the function of genes in tumor cells and modulating the tumor microenvironment, including immunity, inflammation, angiogenesis, stem cell niche, etc. Moreover, intratumor microbiome is regulated by anti‐cancer therapies and actively influences therapy response, which could be a therapeutic target or engineered to be a therapy weapon in the clinic. This review highlights the intratumor microbiome as a vital component in the tumor microenvironment, uncovers potential mutual regulatory mechanisms between the tumor microenvironment and intratumor microbiome, and points out the ongoing research directions and drawbacks of the research area, which should broaden our view of microbiome and enlighten further investigation directions.

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“… 122 Certain bacterial species, especially Cutibacterium acnes , can persist in prostatic tissue specimens. 123 , 124 Intracranial tumours, such as glioblastomas 7 and pituitary neuroendocrine tumours (PitNETs), 125 were found to contain intratumoural microorganisms, and the abundance of microorganisms in different subtypes of the PitNETs was also different, which Fusobacteriaceae , Tissierellaceae , and Aerococcaceae were substantially enriched in adrenocorticotropic hormone-secreting PitNET (ACTH-PitNET) tissues and Corynebacteriaceae , S24-7 , Aerococcaceae , Clostridiales , and F16 were more enriched in growth hormone-secreting PitNET (GH-PitNET) tissues. Moreover, EBV was present in the blood system’s tumour cells, such as Burkitt’s lymphoma, Hodgkin’s lymphoma, and some natural killer (NK) and T cell lymphomas.…”

Section: Characteristics Of Intratumoural Microbiotamentioning

confidence: 99%

Intratumoural microbiota: a new frontier in cancer development and therapy

Cao,

Xia,

Tan

et al. 2024

Sig Transduct Target Ther

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Human microorganisms, including bacteria, fungi, and viruses, play key roles in several physiological and pathological processes. Some studies discovered that tumour tissues once considered sterile actually host a variety of microorganisms, which have been confirmed to be closely related to oncogenesis. The concept of intratumoural microbiota was subsequently proposed. Microbiota could colonise tumour tissues through mucosal destruction, adjacent tissue migration, and hematogenic invasion and affect the biological behaviour of tumours as an important part of the tumour microenvironment. Mechanistic studies have demonstrated that intratumoural microbiota potentially promote the initiation and progression of tumours by inducing genomic instability and mutations, affecting epigenetic modifications, promoting inflammation response, avoiding immune destruction, regulating metabolism, and activating invasion and metastasis. Since more comprehensive and profound insights about intratumoral microbiota are continuously emerging, new methods for the early diagnosis and prognostic assessment of cancer patients have been under examination. In addition, interventions based on intratumoural microbiota show great potential to open a new chapter in antitumour therapy, especially immunotherapy, although there are some inevitable challenges. Here, we aim to provide an extensive review of the concept, development history, potential sources, heterogeneity, and carcinogenic mechanisms of intratumoural microorganisms, explore the potential role of microorganisms in tumour prognosis, and discuss current antitumour treatment regimens that target intratumoural microorganisms and the research prospects and limitations in this field.

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Evidence for an intra-tumoral microbiome in pituitary neuroendocrine tumors with different clinical phenotypes

Cited by 6 publications

References 25 publications

Salivary microbiome profiles for different clinical phenotypes of pituitary adenomas by single-molecular long-read sequencing

Salivary microbiome profiles for different clinical phenotypes of pituitary adenomas by single-molecular long-read sequencing

Intratumor microbiome: selective colonization in the tumor microenvironment and a vital regulator of tumor biology

Intratumoural microbiota: a new frontier in cancer development and therapy

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