Evidence for an extrinsic immunogen in effective ribosomal vaccines from Salmonella typhimurium

Abstract

Previous work has indicated that ribosomes isolated from Salmonella typhimurium were highly immunogenic and afforded excellent protection against homologous challenge. Effective protection was obtained also when ribonucleic acid (RNA) extracted from these ribosomes was used as a vaccine. In this investigation ribosomes prepared by another method and washed repeatedly in 1 M NH4Cl lost much of their prophylactic potency and yielded poorly protective

Salivary immunoglobulin A and serum antibodies to Streptococcus mutans ribosomal preparations in dental caries-free and caries-susceptible human subjects

“…On the other hand, absorption of saliva with S. sanguis ribosomes or NH4Cl-washed S. mutans ribosomes partially depleted antibody activity to S. mutans 6715 ribosomes by approximately 50 and 40%, respectively. NH4Cl reportedly removes cell wall or membrane components adhered to ribosomes (13). Similar results were obtained with pooled serum samples from CF subjects (data not shown).…”

Identification of protective cell surface proteins in ribosomal fractions from Salmonella typhimurium

“…The previous experiments had shown that some of the protective immunogen could be removed either by high-I salt wash or by column chromatography. Since ribosomal fractions had been shown to be contaminated with cell surface materials (8,12,13,18,31), various preparations of cell wall antigens were tested for their immunogenicity in mice. C3H/HeDub mice were significantly protected Symbols: 0, 6707 ribosomes; 0, SR-il ribosomes.…”

“…Earlier experiments have shown that the ribosomal preparations were contaminated with cell surface material (8,12,13,24,31). Although some investigators presume that the protective t Present address: National Institute of Child Health and Human Development, Bethesda, MD 20014. antigen of Salmonella must be a cell surface component, several investigators have shown that both subcellular or ribosomal fractions (8,12,18,30,42,49) and protein extracts from whole cells (3)(4)(5)(6)(7) can protect mice against a lethal Salmonella challenge. This study was undertaken to examine cell surface antigens for their protective ability and any possible association with the protective ribosomal fraction.…”

O‐antigenic specificity of the protective supernatant factor from Salmonella typhimuvium effective in S. typhimurium‐infected mice

“…The need for ribosomal vaccine to contain LPS has been stressed frequently (Hoops et al 1976, Johnson 1972. The importance of 0-specificity was demonstrated by Eisenstein (1975) using ribosomal vaccines from S. enteritidis carrying either its native 0-9 antigen or transduced to carry 0-4 instead.…”

“…Lipopolysaccharide alone neither elicited delayed hypersensitivity reactions nor induced protection (Plant et al 1978) but the necessity for protein in the SF could mean that the LPS must be attached to a protein carrier in order to be effective. The ribosomal preparations of Hoops, Prather, Berry & Ravel (1976) only protected against S. typhimurium infection if they contained traces of lipopolysaccharide. Makela, Valtonen and others previously used S. typhimurium transductants with 0 antigens either 4, 5 (S. typhimurium and B group Salmonellae) or 9 (S. enteritidis and other D group Salmonellae) in virulence experiments (Makela et al 1973, Valtonen 1970, Valtonen et al 1975.…”