Evidence for an Association of Human Papillomavirus and Breast Carcinomas

Damin, Andréa Pires Souto; Karam, Rachid; Zettler, Cláudio Galleano; Caleffi, Maira; Alexandre, Cláudio Osmar Pereira

doi:10.1023/b:brea.0000018411.89667.0d

Cited by 136 publications

(159 citation statements)

References 18 publications

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“…There were no differences in the average of age of women with either HPV-positive and -negative breast cancer in a study of Brazilian women (Damin et al, 2004). This is in contrast to a recent study of Greek women in which those with HPV-positive breast cancer were of average age 38 years as compared to average age 53 years for women with HPV-negative breast cancer (P-values for difference ¼ 0.001) (Kroupis et al, 2006).…”

Section: Sircontrasting

confidence: 63%

Are some breast cancers sexually transmitted?

Iacopetta

et al. 2006

Br J Cancer

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Sir,High-risk human papilloma viruses (HPVs) have been consistently identified in 13 -86% of breast tumours in the 10 studies published since 1999 (Lawson et al, 2006). High-risk HPVs of the same type have been identified in both cervical and breast cancer that had occurred in the same women (Hennig et al, 1999;Widschwendter et al, 2004). This observation has lead to the hypothesis that HPVs may be transmitted to the breast during sexual activities (Kan et al, 2005). If this hypothesis is correct, it is likely that HPV-positive breast cancers would occur in women younger than those with HPV-negative breast cancer. This is because HPV genital infections are much more common in young women who have had multiple sexual partners (IARC, 1995).There are only two studies in which the age of women with HPV-positive and -negative breast cancer has been published. There were no differences in the average of age of women with either HPV-positive and -negative breast cancer in a study of Brazilian women (Damin et al, 2004). This is in contrast to a recent study of Greek women in which those with HPV-positive breast cancer were of average age 38 years as compared to average age 53 years for women with HPV-negative breast cancer (P-values for difference ¼ 0.001) (Kroupis et al, 2006).We have reviewed the ages of Australian women with HPVpositive and -negative breast cancer in our study published in this Journal (Kan et al, 2005). These data are shown in Table 1. The average age of women with HPV-positive breast cancer was 55.6 years as compared to 63.8 years for women with HPV-negative breast cancer (P-values for difference ¼ 0.049). These data are compatible with the hypothesis that HPV-positive breast cancers occur in younger women than those with HPV-negative breast cancers, and that high-risk HPVs may have been transmitted by sexual activity with HPV-positive sexual partners. HPV ¼ human papilloma virus. P-value for difference in average ages ¼ 0.049, which is significant at the 95% level. REFERENCES

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Section: Sircontrasting

confidence: 63%

Are some breast cancers sexually transmitted?

Iacopetta

et al. 2006

Br J Cancer

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“…Many studies have reported the presence of HPV DNA in breast tissues, while others did not confirm the findings [1]. Damin et al found 25% of HPV positivity in 101 breast cancer tissues [14]; moreover, Kroupis et al [6] showed a 16% rate of HPV positivity in more than 100 breast cancer tissue specimens, and they suggested a correlation between infection and elevated tumour grading and proliferation index over a low ER+ expression. However, Lindel et al [26] did not support these findings.…”

Section: Discussionmentioning

confidence: 98%

“…Low or high prevalence of HPV DNA in breast cancer of women was found in several studies. HPV16 or HPV18 were preferentially found in European women, while other types, e.g., 33, were frequently found in Japanese and Chinese cohorts [6][7][8][9][10][11][12][13][14][15][16]. De Villiers et al have recently shown that a broad spectrum of mucosal and cutaneous HPV types could be found in breast specimens using several PCR-based methods [16].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the presence of cutaneous and mucosal papillomavirus types in ductal lavage fluid, milk and colostrum to evaluate its role in breast carcinogenesis

Cazzaniga

Gheit

Casadio

et al. 2008

Breast Cancer Res Treat

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“…Human papilloma virus 33 has been identified in breast cancer in Chinese and Japanese women (Yu et al, 1999). Human papilloma virus 11, 16 and 18 have been identified in breast cancer in US and separately in Brazilian women (Liu et al, 2001;Damin et al, 2004). No HPVs have been identified in normal breast tissues from normal women who have had cosmetic surgery.…”

mentioning

confidence: 99%

Identification of human papillomavirus DNA gene sequences in human breast cancer

et al. 2005

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Human papilloma viruses (HPVs) are accepted as being carcinogenic in human cervical and anogenital cancers. The suspicion that HPVs may also have a role in human breast cancer is based on the identification of HPVs in human breast tumours and the immortalisation of normal human breast cells by HPV types 16 and 18. For this investigation, DNA that had been previously extracted and fresh frozen at À701C from 50 unselected invasive ductal breast cancer specimens were screened by polymerase chain reaction (PCR) for HPV type 16, 18 and 33 gene sequences. We show that HPV 18 gene sequences are present in DNA extracted from breast tumours in Australian women. Overall, 24 (48%) of the 50 samples were HPV positive. Overall no correlations with tumour grade, patient survival, steroid receptor status, ERB-2, p53 expression and mutation were observed. Human papilloma viruses may have a role in human breast cancer. We speculate that HPVs may be transmitted by hand from the female perineum to the breast. It is accepted that human papillomavirus (HPV) types 16 and 18 are carcinogenic, and that probably HPV types 31 and 33 are also carcinogenic in human cervical and anogenital cancers (IARC, 1995). The suspicion that HPVs may also have a role in human breast cancer is based on the identification of HPVs in human breast tumours and the immortalisation of normal human breast cells by HPV 16 and 18 (Band et al, 1990;De Villiers et al, 2005).Human papilloma virus 16 has been identified in breast tumours in Italian women and breast tumours in Norwegian women who had previous cervical neoplasia (Hennig et al, 1999). Human papilloma virus 33 has been identified in breast cancer in Chinese and Japanese women (Yu et al, 1999) MATERIALS AND METHODSFor this investigation, DNA that had been previously extracted and fresh frozen at À70 O C from 50 unselected invasive ductal breast cancer specimens were screened by polymerase chain reaction (PCR) for HPV type 16, 18 and 33 gene sequences. The DNA samples were amplified twice per sample using GenomiPhit DNA Amplification Kit (Amersham Biosciences). The DNA quality was confirmed by PCR, amplifying 268 bp of the b-globin gene. These samples were then screened for the presence of HPV by PCR using primers that could detect 140 bp in the E6 region of HPV 16, 18 and 33 (Yu et al, 1999). DNA extracted from cervical cancer cell lines HeLa and SiHa cells were used as positive controls for HPV 18 and 16, respectively. Plasmid plink 322 HPV 33 was used as a positive control for HPV 33. DNA from leukaemia Raji cells was used a negative control. The PCR products were separated on 7.6% PAGE and visualised by SYBR Green I (Molecular Probes). The screening was repeated five times using different batches of DNA samples amplified by the GenomiPhit DNA Amplification Kit (Amersham Biosciences). Human papilloma virus-positive samples were sequenced (there was sufficient material to sequence 18 of 24 HPV-positive samples).Grade of tumour and survival of patients were known for each sample. Screening for exons 5...

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Evidence for an Association of Human Papillomavirus and Breast Carcinomas

Cited by 136 publications

References 18 publications

Are some breast cancers sexually transmitted?

Are some breast cancers sexually transmitted?

Analysis of the presence of cutaneous and mucosal papillomavirus types in ductal lavage fluid, milk and colostrum to evaluate its role in breast carcinogenesis

Identification of human papillomavirus DNA gene sequences in human breast cancer

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