Evidence for a synergistic effect of post‐translational modifications and genomic composition of eEF‐1α on the adaptation of <i>Phytophthora infestans</i>

Wang, Yanping; Wu, E‐Jiao; Lurwanu, Yahuza; Ding, Jipeng; He, Dun-Chun; Waheed, Abdul; Nkurikiyimfura, Oswald; Liu, Shiting; Li, Wenyang; Wang, Zonghua; Yang, Lina; Zhan, Jiasui

doi:10.1002/ece3.7442

Cited by 2 publications

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References 88 publications

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“…This is much higher than the genetic variation of other functional genes in this species. For example, we only identified < 10 nucleotide haplotypes in the 165 eEF1a sequences ( Wang et al, 2021b ) and two nucleotide haplotypes in the 140 ATP6 sequences ( Zhang et al, 2017 ). However, the genetic variation found in Pi02860 is much lower than other effector genes of the pathogen such as Avr1, Avr2 , Avr3a and Avr4 ( Yang et al, 2018 , 2020 ; Shen et al, 2021 ; Waheed et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic variation along an altitudinal gradient in the Phytophthora infestans effector gene Pi02860

Yang

Ouyang²,

Nkurikiyimfura³

et al. 2022

Front. Microbiol.

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Effector genes, together with climatic and other environmental factors, play multifaceted roles in the development of plant diseases. Understanding the role of environmental factors, particularly climate conditions affecting the evolution of effector genes, is important for predicting the long-term value of the genes in controlling agricultural diseases. Here, we collected Phytophthora infestans populations from five locations along a mountainous hill in China and sequenced the effector gene Pi02860 from >300 isolates. To minimize the influence of other ecological factors, isolates were sampled from the same potato cultivar on the same day. We also expressed the gene to visualise its cellular location, assayed its pathogenicity and evaluated its response to experimental temperatures. We found that Pi02860 exhibited moderate genetic variation at the nucleotide level which was mainly generated by point mutation. The mutations did not change the cellular location of the effector gene but significantly modified the fitness of P. infestans. Genetic variation and pathogenicity of the effector gene were positively associated with the altitude of sample sites, possibly due to increased mutation rate induced by the vertical distribution of environmental factors such as UV radiation and temperature. We further found that Pi02860 expression was regulated by experimental temperature with reduced expression as experimental temperature increased. Together, these results indicate that UV radiation and temperature are important environmental factors regulating the evolution of effector genes and provide us with considerable insight as to their future sustainable action under climate and other environmental change.

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Section: Discussionmentioning

confidence: 99%

Genetic variation along an altitudinal gradient in the Phytophthora infestans effector gene Pi02860

Yang

Ouyang²,

Nkurikiyimfura³

et al. 2022

Front. Microbiol.

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“…The conserved evolution, together with significant difference in the fitness components among the haplotypes suggests the ATP6 is under strong natural selection to purge its genetic variation, consistent with the theoretical expectation for genome essential to maintain basic cellular functions [ 70 ]. The conserved evolution in housekeeping genes has been reported in the concerned pathogen [ 71 ] as well as other species across kingdoms [ 72 – 74 ].…”

Section: Discussionmentioning

confidence: 99%

Fitness difference between two synonymous mutations of Phytophthora infestans ATP6 gene

Nkurikiyimfura,

Waheed,

Fang

et al. 2024

BMC Ecol Evo

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Background Sequence variation produced by mutation provides the ultimate source of natural selection for species adaptation. Unlike nonsynonymous mutation, synonymous mutations are generally considered to be selectively neutral but accumulating evidence suggests they also contribute to species adaptation by regulating the flow of genetic information and the development of functional traits. In this study, we analysed sequence characteristics of ATP6, a housekeeping gene from 139 Phytophthora infestans isolates, and compared the fitness components including metabolic rate, temperature sensitivity, aggressiveness, and fungicide tolerance among synonymous mutations. Results We found that the housekeeping gene exhibited low genetic variation and was represented by two major synonymous mutants at similar frequency (0.496 and 0.468, respectively). The two synonymous mutants were generated by a single nucleotide substitution but differed significantly in fitness as well as temperature-mediated spatial distribution and expression. The synonymous mutant ending in AT was more common in cold regions and was more expressed at lower experimental temperature than the synonymous mutant ending in GC and vice versa. Conclusion Our results are consistent with the argument that synonymous mutations can modulate the adaptive evolution of species including pathogens and have important implications for sustainable disease management, especially under climate change.

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Evidence for a synergistic effect of post‐translational modifications and genomic composition of eEF‐1α on the adaptation of Phytophthora infestans

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 2 publications

References 88 publications

Genetic variation along an altitudinal gradient in the Phytophthora infestans effector gene Pi02860

Genetic variation along an altitudinal gradient in the Phytophthora infestans effector gene Pi02860

Fitness difference between two synonymous mutations of Phytophthora infestans ATP6 gene

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