Evidence for a Stable Intermediate in Leukemia Virus Activation in AKR Mouse Embryo Cells

Kenney, Francis T.; Tennant, Raymond W.

doi:10.1128/jvi.14.3.451-456.1974

Cited by 8 publications

(2 citation statements)

References 24 publications

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“…Production of progeny virus by cells infected with retroviruses requires that the cells pass through one normal replicative cell cycle after infection (5)(6)(7)15). Cells infected in the nonreplicative phase of the cell cycle (stationary cells) do not synthesize viral RNA, viral proteins, or infectious virus until some time after a replicative cell cycle is initiated.…”

mentioning

confidence: 99%

Inhibition of viral DNA synthesis in stationary chicken embryo fibroblasts infected with avian retroviruses

Fritsch¹,

Temin²

1977

J Virol

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Previously, we reported (Fritsch and Temin, J. Virol. 21:119-130, 1977) that infectious viral DNA was not present in spleen necrosis virus-infected stationary chicken cells. However, a stable intermediate was present in such infected stationary cells as evidenced by the appearance of infectious viral DNA shortly after serum stimulation of these cells. After serum stimulation of infected stationary cells, the infectious viral DNA appeared first in the nucleus. In contrast, in infected dividing cells the infectious viral DNA appeared first in the cytoplasm. Significantly reduced amounts of complete plus- or minus-strand viral DNAs were detected by nucleic acid hybridization in stationary chicken cells infected with spleen necrosis virus or Schmidt-Ruppin Rous sarcoma virus compared with the amounts detected in infected dividing cells. These experiments indicated that infected stationary cells did not contain complete noninfectious copies of viral DNA. Furthermore, 5-bromodeoxyuridine labeling and cesium chloride density gradient centrifugation analysis of the infectious viral DNA that appeared after serum stimulation of infected stationary cells indicated that most viral DNA synthesis occurred after addition of fresh serum.

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confidence: 99%

Inhibition of viral DNA synthesis in stationary chicken embryo fibroblasts infected with avian retroviruses

Fritsch¹,

Temin²

1977

J Virol

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“…We have reported that synthetic polyribonucleotides at concentrations . 25 ,ug/ml can neutralize the DEAE-dextranenhanced attachment of radioactively labeled leukemia virus (7). Presumably polyribonucleotides or similarly charged molecules in the cell extracts can have a similar effect.…”

Section: Discussionmentioning

confidence: 98%

Genetic evidence for a product of the Fv-1 locus that transfers resistance to mouse leukemia viruses

Tennant¹,

Schluter²,

Myer³

et al. 1976

J Virol

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Extracts of mouse cells have been shown to transfer resistance to Nor Btropic host range types of mouse leukemia viruses. The genetic specificity of the inhibition was tested in two ways: (i) by correlating the Fv-1 genotype of a number of mouse strains with the restriction-transferring activity of extracts of the respective embryo cell cultures, and (ii) by correlating the Fv-1 genotype of B6C3F2 (C57BL/6 Y [Fv-lbb] by C3H d [Fv-101] parental strains) mouse embryos, which segregate the Fv-1 alleles in a 1:2:1 ratio, with the inhibitor activity of extracts of the cells from each embryo. Five independent matings, totaling 45 individual embryos, were tested. Each embryo was cultured, and the Fv-1 genotype was determined independently by titration of N-and' B-tropic viruses; the extracts of replicate secondary cultures were tested for their effect on infection of permissive cells by N-and B-tropic viruses. The specific-restrictiontransferring activity of the embryos was found to segregate with the appropriate Fv-1 genotype. These results confirm the suggestion that the inhibitor of the leukemia virus host range types in the cellular extracts is a product of the Fv-1 locus.

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Interactions of chemical inducers and steroid enhancers of endogenous mouse type-C RNA viruses

1975

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Evidence for a Stable Intermediate in Leukemia Virus Activation in AKR Mouse Embryo Cells

Cited by 8 publications

References 24 publications

Inhibition of viral DNA synthesis in stationary chicken embryo fibroblasts infected with avian retroviruses

Inhibition of viral DNA synthesis in stationary chicken embryo fibroblasts infected with avian retroviruses

Genetic evidence for a product of the Fv-1 locus that transfers resistance to mouse leukemia viruses

Interactions of chemical inducers and steroid enhancers of endogenous mouse type-C RNA viruses

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