Evidence for a Role of Gibberellins in Salicylic Acid-Modulated Early Plant Responses to Abiotic Stress in Arabidopsis Seeds

Alonso-Ramírez, Ana; Rodrı́guez, Dolores; Reyes, David; Jiménez, Jesús Ángel; Nicolás, G.; López-Climent, María F.; Gómez-Cádenas, Aurelio; Nicolás, Carlos

doi:10.1104/pp.109.139352

Cited by 309 publications

(215 citation statements)

References 46 publications

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“…Both proteins play a role in plant hormone signaling pathways involved in the plant response to biotic stress. Thus, citrate might partially repress the GA signaling pathway, which could also explain the similarity to the transcriptome response to pathogens, as GA not only regulates important plant growth responses but also interacts with other plant hormone signaling pathways and, for example, stimulates the production of SA (Alonso-Ramírez et al, 2009). Furthermore, BRs also inhibit flg22 responses and promote the susceptibility of Arabidopsis to P. syringae (Albrecht et al, 2012;Belkhadir et al, 2012), which may explain why the BR-hypersensitive bak1 mutant has reduced citrate responses.…”

Section: The Interaction Of Citrate With Plant Hormone Signaling Pathmentioning

confidence: 99%

Transcriptomic Analysis of the Role of Carboxylic Acids in Metabolite Signaling in Arabidopsis Leaves

et al. 2013

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The transcriptional response to metabolites is an important mechanism by which plants integrate information about cellular energy and nutrient status. Although some carboxylic acids have been implicated in the regulation of gene expression for select transcripts, it is unclear whether all carboxylic acids have the same effect, how many transcripts are affected, and how carboxylic acid signaling is integrated with other metabolite signals. In this study, we demonstrate that perturbations in cellular concentrations of citrate, and to a lesser extent malate, have a major impact on nucleus-encoded transcript abundance. Functional categories of transcripts that were targeted by both organic acids included photosynthesis, cell wall, biotic stress, and protein synthesis. Specific functional categories that were only regulated by citrate included tricarboxylic acid cycle, nitrogen metabolism, sulfur metabolism, and DNA synthesis. Further quantitative real-time polymerase chain reaction analysis of specific citrate-responsive transcripts demonstrated that the transcript response to citrate is time and concentration dependent and distinct from other organic acids and sugars. Feeding of isocitrate as well as the nonmetabolizable citrate analog tricarballylate revealed that the abundance of selected marker transcripts is responsive to citrate and not downstream metabolites. Interestingly, the transcriptome response to citrate feeding was most similar to those observed after biotic stress treatments and the gibberellin biosynthesis inhibitor paclobutrazol. Feeding of citrate to mutants with defects in plant hormone signaling pathways did not completely abolish the transcript response but hinted at a link with jasmonic acid and gibberellin signaling pathways. Our results suggest that changes in carboxylic acid abundances can be perceived and signaled in Arabidopsis (Arabidopsis thaliana) by as yet unknown signaling pathways.

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Section: The Interaction Of Citrate With Plant Hormone Signaling Pathmentioning

confidence: 99%

Transcriptomic Analysis of the Role of Carboxylic Acids in Metabolite Signaling in Arabidopsis Leaves

et al. 2013

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“…32 Beech FsGASA4 stimulates the expression of salicylic acid (SA) synthetic genes and the plants SA levels to response and resistance to the abiotic stress. 43 In Arabidopsis, AtGASA5 participate in response to heat stress by regulating the SA signaling and the accumulation of heat shock protein. 13 GASAs function in biotic and abiotic stresses and redox reaction Small molecule cysteine-rich antimicrobial peptide as a natural defensive barrier plays an important role in plant disease resistance.…”

Section: Gasas Involve In Plant Growth and Development Processmentioning

confidence: 99%

One new kind of phytohormonal signaling integrator: Up-and-coming GASA family genes

Zhang

Wang

2017

Plant Signaling & Behavior

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GASA proteins are characterized by an N-terminal signal peptide and a C-terminal conserved GASA domain with 12 invariant cysteine residues. Despite being widely distributed among plant species, their functions are not completely elucidated and little is known about their mechanism of action. This review focuses on the current knowledge about the molecular structure, protein subcellular localization and phytohormones responses of this up-and-coming family of peptides. Furthermore, we discussed the roles of GASA proteins in plant growth and development, plant responses to biotic or abiotic stresses and their participation in phytohormonal signaling integration.

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“…Furthermore, this hypothesis is supported by the fact that sid2 mutants, impaired in SA biosynthesis, are more sensitive to salt stress than wild type and are not affected by exogenous application of GA 3 . (Alonso-Ramírez et al 2009). In order to study the regulation of exogenous GA 3 (80 µM) on the growth of Zoysia japonica under abiotic stresses, the survival rate of Zoysia japonica seedlings was statisticsed in different stress time.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of gibberellin 20 oxidase gene induced by abiotic stresses in Zoysiagrass (Zoysia japonica)

Dai

Cheng

et al. 2012

Biologia

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The gibberellin 20-oxidase gene (GA20) plays an important role in plant growth and development. Differential expression of Zoysiagrass (Zoysia japonica Steud.) gibberellin 20 oxidase gene (ZjGA20) induced by abiotic stresses has not been reported. In this investigation, we first reported the differential expression of ZjGA20 in different Z. japonica tissues including root, young leaf, senescent leaf, blade, sheath, and stolon, as well as differential expression induced by abiotic stresses including low temperature (4 • C), H2O2(8 µM), salt stress (250 mM NaCl), 25% PEG6000, and high temperature (42 • C) by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Higher expression of ZjGA20 was observed in young leaf and sheath, compared to root, senescent leaf, blade, and stolon. Among different abiotic stresses, expression of ZjGA20 decreased under low temperature, 25% PEG6000, and high temperature. The highest expression of ZjGA20 was obtained when plants were treated with 8 µM H2O2 for 10 h and with 250 mM NaCl for 5 h. The analysis of the MDA content, POD activity, and permeability of the plasma membrane demonstrated that application of exogenous GA3 recovered tissue damage derived from low temperature treatment. In addition, the expression of ZjGA20 increased under low temperature stress. These results demonstrated that expression of ZjGA20 was regulated by abiotic stresses and the damage derived from abiotic stresses could be rescued by exogenously applied plant growth regulator GA3. Further more, exogenous gibberellin and salicylic acid (SA) alleviated the growth inhibition and death of the seedlings under stresses. The SA content in the seedlings treated with 80 µM GA3 was far greater than the control (with H2O) and plants under stress treatments. These data suggest that exogenous addition of GA3 is able to counteract the inhibitory effects of these adverse environmental conditions in Zoysiagrass growth through modulation of SA biosynthesis. This is the first study of differential expression of gibberellin 20 oxidase gene and growth regulation of GA3 in Zoysiagrass under stresses.

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Evidence for a Role of Gibberellins in Salicylic Acid-Modulated Early Plant Responses to Abiotic Stress in Arabidopsis Seeds

Cited by 309 publications

References 46 publications

Transcriptomic Analysis of the Role of Carboxylic Acids in Metabolite Signaling in Arabidopsis Leaves

Transcriptomic Analysis of the Role of Carboxylic Acids in Metabolite Signaling in Arabidopsis Leaves

One new kind of phytohormonal signaling integrator: Up-and-coming GASA family genes

Differential expression of gibberellin 20 oxidase gene induced by abiotic stresses in Zoysiagrass (Zoysia japonica)

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