Evidence for a resident subset of cells with SP phenotype in the C2C12 myogenic line: a tool to explore muscle stem cell biology

Benchaouir, Rachid; Rameau, Philippe; Decraene, Charles; Dreyfus, Patrick A.; Israeli, David; Piétu, Geneviève; Danos, Olivier; Garcı́a, Luis

doi:10.1016/j.yexcr.2003.11.005

Cited by 56 publications

(70 citation statements)

References 39 publications

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“…These data do not necessarily challenge the conclusion that SP cells could be used to isolate cancer stem cells, but confirm that NSP cells had the ability of asymmetric division, which was consistence with several previous results. 21,22 Stem cells and progenitor cells show anchorage-independent growth, which is a property related to self-renewal. 23 Cell lines should contain stem cells and progenitor cells regardless of whether they form colonies.…”

Section: Discussionmentioning

confidence: 99%

Identification of side population cells from bladder cancer cells by DyeCycle Violet staining

She

Zhang²,

Wang³

et al. 2008

Cancer Biology & Therapy

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Section: Discussionmentioning

confidence: 99%

Identification of side population cells from bladder cancer cells by DyeCycle Violet staining

She

Zhang²,

Wang³

et al. 2008

Cancer Biology & Therapy

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“…Especially, the recent reports have shown that ectopic expression of msx1 in C2C12 myotubes can induce dedifferentiation of the myotubes to produce multipotent mononucleated cells (Odelberg et al, 2000), and a 2,6-disubstituted purine, reversine, can also induce dedifferentiation of C2C12 myoblasts to become mesenchymal progenitor cells (Chen et al, 2004). However, the C2C12 myogenic line may be heterogeneous because there is this novel evidence for a resident subset of cells with side population (SP) phenotype in the C2C12 line (Benchaouir et al, 2004), whereas SP cells themselves are growth-arrested and delayed in their ability to differentiate with behaving as multipotent stem cells (Zhou et al, 2001;Jankowski et al, 2002;Tamaki et al, 2003). In addition, whether human skeletal myoblasts can dedifferentiate is unclear.…”

mentioning

confidence: 99%

Dedifferentiation of Adult Human Myoblasts Induced by Ciliary Neurotrophic Factor In Vitro

Chen

Mao

Liu³

et al. 2005

MBoC

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Ciliary neurotrophic factor (CNTF) is primarily known for its important cellular effects within the nervous system. However, recent studies indicate that its receptor can be highly expressed in denervated skeletal muscle. Here, we investigated the direct effect of CNTF on skeletal myoblasts of adult human. Surprisingly, we found that CNTF induced the myogenic lineage-committed myoblasts at a clonal level to dedifferentiate into multipotent progenitor cells-they not only could proliferate for over 20 passages with the expression absence of myogenic specific factors Myf5 and MyoD, but they were also capable of differentiating into new phenotypes, mainly neurons, glial cells, smooth muscle cells, and adipocytes. These "progenitor cells" retained their myogenic memory and were capable of redifferentiating into myotubes. Furthermore, CNTF could activate the p44/p42 MAPK and down-regulate the expression of myogenic regulatory factors (MRFs). Finally, PD98059, a specific inhibitor of p44/p42 MAPK pathway, was able to abolish the effects of CNTF on both myoblast fate and MRF expression. Our results demonstrate the myogenic lineage-committed human myoblasts can dedifferentiate at a clonal level and CNTF is a novel regulator of skeletal myoblast dedifferentiation via p44/p42 MAPK pathway. INTRODUCTIONAdult skeletal myoblasts have long been considered as myogenic lineage-committed cells with either self-renewing or differentiating into multinucleated myotubes in vitro (Jankowski et al., 2002;Tamaki et al., 2003). Myoblast fate is critically dependent on the myogenic regulatory factors (MRFs): MyoD, Myf5, myogenin, and Mrf4, which have a well-defined role in orchestrating skeletal muscle development and differentiation. Gene targeting in mice has illustrated the essential role for MRFs: Myf5 and MyoD is critical to establishing the myogenic cell lineage and producing committed, undifferentiated myoblasts (Kablar et al., 1999), whereas myogenin has an important role in initiating differentiation (Hasty et al., 1993); Mrf4 regulates terminal differentiation and myofiber maintenance (Patapoutian et al., 1995). Gene targeting in mice has illustrated the essential role for Myf5 and MyoD in myoblast fates. In MyoDϪ/Ϫ and/or Myf5Ϫ/Ϫ knockout mice, muscle precursors acquire nonmuscle cell fates (Kablar et al., 1999) and become multipotential stem cells or a progenitor compartment (Parker et al., 2003). Early histological studies indicated that muscle fibers at the amputation plane of urodele amphibians might dedifferentiate by budding off mononucleate cells from syncytial muscle cells (Echeverri and Tanaka, 2002). Recent studies suggest that dedifferentiation may be also possible in mammalian system (Odelberg et al., 2000;Tsai et al., 2002;Chen et al., 2004). Especially, the recent reports have shown that ectopic expression of msx1 in C2C12 myotubes can induce dedifferentiation of the myotubes to produce multipotent mononucleated cells (Odelberg et al., 2000), and a 2,6-disubstituted purine, reversine, can also induce dedifferentia...

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“…After trauma or damage, MPCs drive muscle tissue regeneration by proliferating and differentiating into myoblasts, further fusing and eventually forming new myofibers. While entire muscle naturally contains cells of different origins, including from the vascular and hematopietic compartment, MPCs represent a subset of muscle cells that are generally committed to the myogenic lineage (Benchaouir et al, 2004). Therefore, MPCs are of particular interest for muscle engineering, and may be a valuable alternative to more original smooth muscle cells in order to restore function of defective or absent anal sphincter.…”

Section: Progenitor and Stem Cells 2231 Muscle Progenitor And Stemmentioning

confidence: 99%

Bioengineering of Colo-Rectal Tissue

Inglin¹,

Brügger²,

Candinas³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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Evidence for a resident subset of cells with SP phenotype in the C2C12 myogenic line: a tool to explore muscle stem cell biology

Cited by 56 publications

References 39 publications

Identification of side population cells from bladder cancer cells by DyeCycle Violet staining

Identification of side population cells from bladder cancer cells by DyeCycle Violet staining

Dedifferentiation of Adult Human Myoblasts Induced by Ciliary Neurotrophic Factor In Vitro

Bioengineering of Colo-Rectal Tissue

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