Evidence for a novel P2X purinoceptor in human placental chorionic surface arteries

Dobronyi, I.; Hung, Kuen‐Shan; Satchell, D.G.; Maguire, M. Helen

doi:10.1016/s0014-2999(96)00967-3

Cited by 12 publications

(6 citation statements)

References 13 publications

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Section: N Human Umbilical and Placental Vesselsmentioning

confidence: 89%

“…Interestingly, contractile responses to ATP and a,b-meATP were sustained in human perfused placental cotyledons and human placental chorionic surface arteries (Dobronyi et al, 1997;Ralevic et al, 1997) in contrast with the rapidly desensitizing responses observed to a,b-meATP via P2X 1 receptors in most other blood vessels. Furthermore, responses to ATP and a,b-meATP were blocked by suramin and insensitive to PPADS (a P2 receptor antagonist that blocks P2X 1 responses in other vessels), whereas UTP elicited little or no contraction, which might indicate the involvement of heteromeric P2X receptors (Dobronyi et al, 1997;Ralevic et al, 1997). An elegant study by Buvinic et al (2006) showed that P2Y 1 and P2Y 2 (or P2Y 4 ) receptors were coupled to both contraction and relaxation in human perfused placental cotyledons and that these receptors were unevenly distributed along the placental vascular tree; in the chord and chorionic vessels P2Y 1 and P2Y 2 receptor distribution was mainly in the smooth muscle, whereas in the cotyledon vessel these receptors were equally distributed between the endothelium and smooth muscle cells (Buvinic et al, 2006).…”

Section: N Human Umbilical and Placental Vesselsmentioning

confidence: 89%

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Purinergic Signaling and Blood Vessels in Health and Disease

2013

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Section: N Human Umbilical and Placental Vesselsmentioning

confidence: 89%

Section: N Human Umbilical and Placental Vesselsmentioning

confidence: 89%

Purinergic Signaling and Blood Vessels in Health and Disease

2013

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“…This was an unexpected finding, as in most arteries prolonged exposure to α,β‐meATP induces P2X receptor desensitization (Kennedy et al ., 1997). Non‐desensitizing responses to α,β‐meATP have been reported in only a few cases; in the human placenta arterial bed (Dobronyi et al ., 1997; Ralevic et al ., 1997) and rat renal vessels (Inscho et al ., 1998; van der giet et al ., 1999).…”

Section: Discussionmentioning

confidence: 99%

“…This was an unexpected ®nding, as in most arteries prolonged exposure to a,b-meATP induces P2X receptor desensitization (Kennedy et al, 1997). Nondesensitizing responses to a,b-meATP have been reported in only a few cases; in the human placenta arterial bed (Dobronyi et al, 1997;Ralevic et al, 1997) and rat renal vessels (Inscho et al, 1998;Van Der Giet et al, 1999). Which P2X receptor subunit(s) mediates these maintained responses is unclear as a,b-meATP is not an agonist at the non-desensitizing recombinant P2X receptors (P2X 2,4,5,6,7 ) (Khakh et al, 2001).…”

Section: P2x Receptors In Rat Pulmonary Arteriesmentioning

confidence: 93%

Regional variation in P2 receptor expression in the rat pulmonary arterial circulation

Chootip

Ness

Wang

et al. 2002

British J Pharmacology

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1 The P2 receptors that mediate contraction of the rat isolated small (SPA, 200 ± 500 mm i.d.) and large (LPA, 1 ± 1.5 mM i.d.) intrapulmonary arteries were characterized. 2 In endothelium-denuded vessels the contractile order of potency was a,b-methyleneATP (a,bmeATP)44UDP=UTP=ATP=2-methylthioATP4ADP in the SPA and a,b-meATP=UT-P5UDP42-methylthioATP, ATP44ADP in the LPA. a,b-meATP, 2-methylthioATP and ATP had signi®cantly greater e ects in the SPA than the LPA (P50.001), but there was no di erence in the potency of UTP or UDP between the vessels. 3 In the SPA, P2X 1 receptor desensitisation by a,b-meATP (100 mM) inhibited contractions to a,bmeATP (10 nM ± 300 mM), but not those to UTP or UDP (100 nM ± 300 mM). In the LPA, prolonged exposure to a,b-meATP (100 mM) did not desensitize P2X receptors. 4 Pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), suramin and reactive blue 2 (RB2) (30 ± 300 mM) inhibited contractions evoked by a,b-meATP. UTP and UDP were potentiated by PPADS, una ected by RB2 and inhibited, but not abolished by suramin. 1 and 3 mM suramin produced no further inhibition, indicating suramin-resistant components in the responses to UTP and UDP. 5 Thus, both P2X and P2Y receptors mediate contraction of rat large and small intrapulmonary arteries. P2Y agonist potency and sensitivity to antagonists were similar in small and large vessels, but P2X agonists were more potent in small arteries. This indicates di erential expression of P2X, but not P2Y receptors along the pulmonary arterial tree.

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“…97,98 Indeed, some of the preliminary results of these studies showed that purinergic signaling agonists can elicit reproducible and potent contractions of the circular layer of rings of the chorionic vessels, as well as perfused cotyledons from the human placenta. [99][100][101] However, the exact mechanism of action of extracellular nucleotides with circulating leukocytes and vascular endothelium and smooth muscle cells of the placenta vasculature still remains unknown.…”

Section: The Involvement Of E Atp In the Immune Re S P On S E A S Amentioning

confidence: 99%

Inflammation—The role of ATP in pre‐eclampsia

2019

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Sterile inflammation may be initiated by molecules in the host organism that signal “damage” or “danger” also known as danger‐associated molecular pattern (DAMPs). In pre‐eclampsia (PE), a variety of DAMPs may be involved in the etiology or exacerbation of the disorder. Adenosine 5’‐triphosphate (ATP) is a key intracellular energy molecule as well as a ligand for purinergic receptors. In humans, under physiological conditions, extracellular ATP (eATP) levels are distinctly low, but can rise to several hundred fold when cells become injured, stressed, or even necrotic. This often initiates a sterile inflammatory response with eATP acting as a DAMP. Extracellular ATP and its derivative nucleotides synthetized by endonucleotidases exhibit many of their effects through purinergic receptors, via inflammatory cascades and the production of proinflammatory molecules. This is clearly seen in the P2X7 gated receptor, which is linked to release of cytokines of the interleukin‐1 family. Considering its fundamental role in innate immunity, an imbalance of P2X7 receptor activation may lead to deleterious effects in the coordination of placental vessel tone via the synthesis of various proinflammatory cytokines. This review explores the implication of DAMPs, specifically ATP and uric acid in the inflammation associated with PE.

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Evidence for a novel P2X purinoceptor in human placental chorionic surface arteries

Cited by 12 publications

References 13 publications

Purinergic Signaling and Blood Vessels in Health and Disease

Purinergic Signaling and Blood Vessels in Health and Disease

Regional variation in P2 receptor expression in the rat pulmonary arterial circulation

Inflammation—The role of ATP in pre‐eclampsia

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