Evidence for a mechanical coupling of glycoprotein microfibrils with collagen fibrils in Wharton's jelly

Meyer, F. A.; Laver-Rudich, Z.; Tanenbaum, R

doi:10.1016/0304-4165(83)90241-6

Cited by 70 publications

(48 citation statements)

References 39 publications

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“…However, Wharton's jelly of human umbilical cord is known to contain hyaluronic acid and sulphated glycosaminoglycans immobilized in an insoluble microfibril network. Collagenase II is stronger for its clostripain activity, which is more efficient in solubilizing the UC microfibrils than other types of collagenase (Meyer et al 1983). Previous study suggest that degradation of the extracellular matrix and disintegration of cell membranes may have caused cellular damage through the use of trypsin alone for a long period, because some cells are sensitive to exposure to trypsin but not to collagenase (Oyama et al 1990).…”

Section: Discussionmentioning

confidence: 99%

Optimization of human umbilical cord mesenchymal stem cell isolation and culture methods

et al. 2013

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Human umbilical cord mesenchymal stem cells (hUCMSCs) are considered to be an ideal replacement for bone marrow MSCs. However, up to date, there is no convenient and efficient method for hUCMSC isolation and culture. The present study was carried out to explore the modified enzyme digestion for hUCMSC in vitro. Conventional enzyme digestion, modified enzyme digestion, and tissue explant were used on hUCMSCs to compare their efficiencies of isolation and culture, to observe primary cell growth and cell subculture. The results show that the cells cultured using the tissue explant method had a longer culture cycle (P \ 0.01) and lower yield of primary cells per centimetre of umbilical cord (P \ 0.01) compared with the two enzyme digestion methods. Subculture adherence and cell doubling took significantly less time with the tissue explant method (P \ 0.05) than with the conventional enzyme digestion method; however, there was no significant difference between the tissue explant method and the modified enzyme digestion method (P [ 0.05). Comparing two enzyme digestion methods, the modified method yielded more cells than did the conventional method (P \ 0.01), and primary cell adherence took significantly less time with the modified method than with the conventional method (P \ 0.05). Cell cycle analysis of the third-generation hUCMSCs cultured by modified enzyme digestion method indicated that most cells were quiescent. Immunofluorescence staining showed that these cells expressed MSC markers CD44 and CD90. And Von Kossa and oil red O staining detection showed that they could be differentiated into 123 Cytotechnology (2013) 65:819-827 DOI 10.1007 osteoblasts and adipocytes with induction medium in vitro. This study suggests that hUCMSC isolation and culture using 0.2 % collagenase II at 37°C for digestion of 16-20 h is an effective and simple modified enzyme digestion method.

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Section: Discussionmentioning

confidence: 99%

Optimization of human umbilical cord mesenchymal stem cell isolation and culture methods

et al. 2013

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“…The cord is covered by an epithelium derived from the enveloping amnion. The network of glycoprotein microfibrils and collagen fibrils in the Wharton's jelly has been previously studied [1]. The interlaced collagen fibers and small, woven bundles are arranged to form a continuous soft skeleton that encases the umbilical vessels [2].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord

Wang¹,

Hung

Peng³

et al. 2004

STEM CELLS

1,207

960

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The Wharton's jelly of the umbilical cord contains mucoid connective tissue and fibroblast-like cells. Using flow cytometric analysis, we found that mesenchymal cells isolated from the umbilical cord express matrix receptors (CD44, CD105) and integrin markers (CD29, CD51) but not hematopoietic lineage markers (CD34, CD45). Interestingly, these cells also express significant amounts of mesenchymal stem cell markers (SH2, SH3). We therefore investigated the potential of these cells to differentiate into cardiomyocytes by treating them with 5-azacytidine or by culturing them in cardiomyocyteconditioned medium and found that both sets of conditions resulted in the expression of cardiomyocyte markers, namely N-cadherin and cardiac troponin I. We also showed that these cells have multilineage potential and that, under suitable culture conditions, are able to differentiate into cells of the adipogenic and osteogenic lineages. These findings may have a significant impact on studies of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering by helping to eliminate worrying ethical and technical issues.

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“…Umbilical cord at term is made of three vessels (two arteries coiled around a vein) surrounded by the Wharton's jelly, a soft connective tissue composed of few mesenchymal stromal cells and abundant extracellular matrix ( Fig. 1) (Hoyes 1969;Parry and Abramovich 1972;Meyer et al 1983;Sobolewski et al 1997;Can and Karahuseyinoglu 2007). Human umbilical cord is a special organ subjected to many changes during pre-natal life and whose cells can maintain a certain degree of plasticity also in post-natal period; for example they have wide differentiation potentiality ) and recently have been used as a source of stem cells (Conconi et al 2006;Fu et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and transcriptional expression of matrix metalloproteinases in full-term human umbilical cord and Human Umbilical Vein Endothelial Cells

2010

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Matrix metalloproteinases (MMPs) are extracellular zinc-dependent endopeptidases involved in the degradation and remodelling of extracellular matrix in physiological and pathological processes. MMPs also have a role on cell proliferation, migration, differentiation, angiogenesis and apoptosis. Umbilical cord is a special organ subjected to many changes during pre-natal life and whose cells can maintain a certain degree of plasticity also in post-natal period; for example recently they have been used as a source of stem cells. In this work we investigated the expression of MMPs in human umbilical cord and Human Umbilical Vein Endothelial Cells (HUVEC) though immunohistochemistry, RT-PCR and gelatin zymography. MMP-2 protein is expressed in the amniotic epithelium of human umbilical cord and in few sub-epithelial fibroblasts, while MMP-3 and MMP-10 only in the umbilical epithelium. MMP-8, MMP-9 and MMP-13 immunoreactivity is localised in the epithelium and in Wharton's jelly mesenchymal cells. Immunocytochemistry also revealed protein expression for MMP-2, 3, 8, 9 and 10 in cultured HUVEC. In agreement with immunohistochemical data, RT-PCR analysis performed on samples of whole umbilical cord confirmed the transcriptional expression for the genes encoding all the six matrix metalloproteinases investigated, while in HUVEC only the expression of MMP-2, 3, 9, 10 and 13 mRNAs was detected. Gelatin zymograpgy showed a clear MMP-2 and MMP-9 enzymatic activity in the conditioned medium of HUVEC at different culture passages, suggesting that HUVEC secrete gelatinases, that afterwards undergo extracellular activation, and this ability is not affected by passage number.

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Evidence for a mechanical coupling of glycoprotein microfibrils with collagen fibrils in Wharton's jelly

Cited by 70 publications

References 39 publications

Optimization of human umbilical cord mesenchymal stem cell isolation and culture methods

Optimization of human umbilical cord mesenchymal stem cell isolation and culture methods

Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord

Immunohistochemical and transcriptional expression of matrix metalloproteinases in full-term human umbilical cord and Human Umbilical Vein Endothelial Cells

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