Evidence for a major cluster of lymphocyte differentiation antigens on murine chromosome 2.

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Several polymorphic DNA restriction endonuclease fragments hybridizing with xenotropic and ecotropic envelope virus probes map adjacent to minor histocompatibility and lymphocyte (H/Ly) antigen-encoding loci. Viral DNA restriction fragments are associated with Ly-17 on chromosome 1, H-30, H-3, and H-13 on chromosome 2, Ly-21 on chromosome 7, H-28 on chromosome 3, and H-38 (chromosomal location as yet undetermined). In each case no recombinant can be found between the H/Ly locus in question and the virus-related restriction fragment, suggesting that linkage is very tight. Although some viral loci map to locations where no H/Ly has yet been mapped, the frequency and tightness of linkage in the seven instances described, coupled with the large number of as yet unmapped H/Ly loci, suggests that the associations found are significant.

mentioning

confidence: 99%

mentioning

confidence: 99%

Association of endogenous viral loci with genes encoding murine histocompatibility and lymphocyte differentiation antigens.

Meruelo¹,

Rossomando²,

Offer³

et al. 1983

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“…The Ly-6 gene complex was initially mapped by genetic techniques to chromosome 9 (15) and was later reassigned to chromosome 2 (16). This paper reports the use of the Ly6E.…”

mentioning

confidence: 99%

Murine Ly-6 multigene family is located on chromosome 15.

LeClair¹,

Rabin²,

Nesbitt³

et al. 1987

Murine Ly-6-encoded molecules play an important role in the antigen-independent activation of lymphocytes. We have described the cloning of a cDNA encoding the protein component of an Ly-6 molecule. Hybridization studies indicated that this cDNA identified multiple DNA fragments on Southern blots. The banding pattern exhibits a restriction fragment length polymorphism from mice bearing either the Ly-6a or the Ly-6b allele. We have employed three independent chromosomal mapping techniques, somatic cell hybrids, in situ hybridization, and strain distribution pattern analysis of the restriction fragment length polymorphism of DNA from recombinant inbred lines, to ascertain the chromosomal origins of these bands. We report that all members of the Ly-6 multigene family are tightly linked on chromosome 15 and have been regionalized by in situ hybridization analysis to band 15E on the distal portion of this chromosome. Linkage analysis has indicated that the Ly-6 genes are located within 1 map unit of Env-54 (a retroviral envelope restriction fragment length polymorphism probe), 3 map units from ins-1, (insulin-related gene), and 4 map units from the protooncogene c-sis. The possible involvement of the Ly-6 lymphocyte activation and differentiation antigen genes in chromosome 15-related lymphoid malignancies is discussed.

“…A growing body of evidence also suggests that a series of cell surface antigens are encoded by genes on the second chromosome (11)(12)(13)(14)(15). At least one of these antigens is a manifestation of j32-microglobulin (32m), and it is possible that and H-3 antigens are also closely related to (32m (16)(17)(18).…”

mentioning

confidence: 99%

A lymphocyte antigen encoded by a locus closely linked to H-3 on the second chromosome of the mouse.

Gasser¹,

Ziebur²,

Matsumoto³

1983

GeneticsA lymphocyte antigen encoded by a locus closely linked to H-3 on the second chromosome of the mouse (monoclonal antibodies/B2-microglobulin linkage) DAVID L. GASSER, JANET ZIEBUR, AND Kozo MATSUMOTO Department of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 Communicated by Werner Henle, July 22, 1983 ABSTRACT A monoclonal antibody has been developed that recognizes a mouse lymphocyte antigen, Ly-23, that is controlled by an H-3 histocompatibility complex-linked gene on the second chromosome. Competitive binding experiments demonstrate that this antigen is distinct from the polymorphic 2-microglobulin antigen, which also is encoded by a gene closely linked to H-3. The strain distribution pattern differs from that of the P2-microglobulin polymorphism, and recombination apparently has separated the genes for these two antigens during the derivation of one recombinant inbred line. However, the RIE/TL8Xline of cells, which lacks (-microglobulin, is negative forthe Ly-23 antigen, although the control RI. 1 line is positive. The H-2 locus affects the expression of both the Ly-23 and &-microglobulin antigens.