Evidence for a dual mechanism of chick embryo fibroblast adhesion on fibronectin and laminin substrata

Codogno, Patrice; Doyennette-Moyne, Marie-Agnès; Auger, Michèle

doi:10.1016/0014-4827(87)90208-4

Cited by 27 publications

(19 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Epidermalization on an extracellular matrix sub strate: Cell culture insert membranes were coated, as described elsewhere [25], with the following extracellu lar matrix components before plating the keratinocytes: laminin (3 pg/cm2) [30], fibronectin (l.5 p g / cm2), type I collagen ( 12 pg/cm2) and type IV collagen (8 pg/cm2). Keratinocyte epidermalization was done in the three experimental conditions described above (i.e.…”

Section: Human Reconstructed Epidermismentioning

confidence: 99%

Barrier Function of Reconstructed Epidermis at the Air-Liquid Interface: Influence of Dermal Cells and Extracellular Components

Robert

Dusser

et al. 1997

Skin Pharmacol Physiol

View full text Add to dashboard Cite

To evaluate the epidermal barrier function of in vitro reconstructed epidermis, we measured the penetration of estradiol and water across human keratinocytes cultured in defined medium (DM), in the presence of proliferative fibroblasts (pF) or conditioned medium derived from pF, at the air-liquid interface on synthetic porous membrane, noncoated or coated with laminin, fibronectin, type I collagen or type IV collagen. Ultrastructural analysis showed a well-developed stratum corneum whatever the culture conditions. The permeability of reconstructed epidermis in DM on a noncoated porous membrane was 5- to 10-fold higher than human native epidermis, with both tracers. No significant change in barrier function was observed whatever the culture conditions.

show abstract

Section: Human Reconstructed Epidermismentioning

confidence: 99%

Barrier Function of Reconstructed Epidermis at the Air-Liquid Interface: Influence of Dermal Cells and Extracellular Components

Robert

Dusser

et al. 1997

Skin Pharmacol Physiol

View full text Add to dashboard Cite

show abstract

“…Adhesion assay and cell spreading FN and LM substrates were prepared as detailed previously (Codogno et al, 1987 (Codogno et al, 1988a). Although, Dean et al (1988) (Lesot et al, 1983) and to the extracellular matrix receptor belonging to the integrin family (Ruoslahti, 1988).…”

mentioning

confidence: 99%

Cell spreading on laminin substrate involves Con A-binding proteins

Moutsita

Botti²,

Doyennette-Moyne³

et al. 1990

Reprod. Nutr. Dévelop.

View full text Add to dashboard Cite

“…As shown by SDS gelelectrophoresis, the protease cleaved a 135 kD cell surface molecule required for adhesion [20]. Low-molecular plasma proteins, mediating cell-fibronectin interactions in chicken embryo fibroblasts, are also sensitive to trypsin treatment [21]. On baby hamster kidney cells a wheat germ agglutinin recep tor.…”

Section: Discussionmentioning

confidence: 99%

Influence of Protease Treatment on the Adhesiveness of Tumor Cells to Extracellular Matrix Components

Uster¹,

Erdmann²,

Rimpler³

1995

Complement Med Res

View full text Add to dashboard Cite

The ability of malignant tumor cells to form secondary lesions depends on multiple adhesive steps during invasion and metastasis. Details are given that a proteolytic treatment of the tumor cell surface causes a decreased attachment of the cells to matrix components. The attachment of murine B16 melanoma cells to the complete matrix (ECM) showed 40% inhibition after a bromelain treatment in comparison to control cells. A reduced binding rate to the isolated matrix components laminin and fibronectin was also found for the melanoma cell line as well as for a human bladder carcinoma (BC) after a proteolytic treatment with bromelain or ficin. Furthermore, the haptotactic migration of pancreatic carcinoma cells (DAN-G cell line) to laminin and fibronectin and that of B16-F1 cells to fibronectin was also influenced by ficin and bromelain, as shown in an in vitro invasion system. Other experiments were performed with arg-gly-asp-containing synthetic peptides (RGD polymer) as attachment protein mimicking the cell-binding domain of many adhesive proteins, which is recognized by several cell surface receptors. A bromelain treatment reduced the RGD attachment of B16 melanoma cells and that of their B16-F1 and B16-F10 variants, which differ in their capacity to colonize the lung. A panel of other proteases, including human elastase and plasmin, assayed in a physiological concentration, showed a similiar adhesion-reducing effect. The chromatogra-phic purification of bromelain resulted in fractions which only influenced the cell binding to the adhesive sequence in a lower extent than the starting material. The ability of the tested proteases to reduce the cell binding to the RGD polymer indicates an influence of the proteolytic treatment on the RGD-dependent integrin system. The findings support the possibility for a therapeutic use of proteases for adjuvant cancer treatment.

show abstract

Evidence for a dual mechanism of chick embryo fibroblast adhesion on fibronectin and laminin substrata

Cited by 27 publications

References 39 publications

Barrier Function of Reconstructed Epidermis at the Air-Liquid Interface: Influence of Dermal Cells and Extracellular Components

Barrier Function of Reconstructed Epidermis at the Air-Liquid Interface: Influence of Dermal Cells and Extracellular Components

Cell spreading on laminin substrate involves Con A-binding proteins

Influence of Protease Treatment on the Adhesiveness of Tumor Cells to Extracellular Matrix Components

Contact Info

Product

Resources

About