Evidence for a differential translocation of actinides across human lung epithelial cell monolayer in vitro according to their physicochemical properties and the presence of a chelating agent

Take a Swipe at Actinide Bioavailability: Application of a New In Vitro Method

“…5 a). In addition, these transfer results are in agreement with dissolution experiments, for in vitro data using different cell-culture systems (macrophages, lung epithelial cells) as well as for in vivo data ( Khokhryakov et al 1998 ; Aladova et al 2007 ; Van der Meeren et al 2012 , 2016 , 2022b ). This latter is a prerequisite for validation of any in vitro system applied to the estimation of bioavailability.…”

“…Recent work from the Laboratory of RadioToxicology in France has addressed the question of new experimental approaches to forecast actinide bioavailability. In this model, different actinides are incorporated into an agarose gel called the static phase and the transfer of activity to the external medium called the dynamic phase is monitored (Griffiths et al 2016; Van der Meeren et al 2019). In this approach both phases may be modified to represent fluid compartments (blood, synovial fluid, sweat) or retention compartments (bone, lungs, intracellular) (Griffiths et al 2016;Van der Meeren et al 2022a).…”

Determination of drug efficacy to dissolve cobalt oxide particles in cellular models: Towards a therapeutic approach to decrease pulmonary retention

“…Asc alone or associated with DFOB does not result in a significant difference in intracellular cobalt accumulation. Interestingly, such a decrease in cellassociated activity in the presence of DTPA has been also observed following Calu-3 contamination with americium (Drouet et al, 2021;Van der Meeren et al, 2021) .…”

“…Calu-3 monolayer grown on filter devices has been widely used to mimic translocation from lungs to blood of various substances (Florea et al, 2003;Foster et al, 2000;George et al, 2015;Haghi et al, 2010;Van der Meeren et al, 2021) . We measured the transfer of the soluble cobalt compound 57 CoCl 2 across Calu-3 monolayers over 7 days to mimic the behavior of the dissolved fraction of cobalt particles.…”

“…, we demonstrated that combining molecules with different properties, such as reducing agents and Cochelating molecules, improved the efficacy of each drug used alone, to dissolve Co 3 O 4 P. In the present work, we used two cellular models previously developed to study the uptake and release of actinides after macrophage phagocytosis(Van der Meeren et al, 2016) and the subsequent translocation across an epithelial monolayer(Van der Meeren et al, 2021) to explore the therapeutic potential of ascorbic acid associated or not with the chelators DTPA, EDTA or DFOB.…”