Evidence-Based Status of Second- and Third-Generation Autologous Chondrocyte Implantation Over First Generation: A Systematic Review of Level I and II Studies

Goyal, Deepak; Goyal, Anjali; Keyhani, Sohrab; Lee, Eng Hin; Hui, James

doi:10.1016/j.arthro.2013.07.271

Cited by 98 publications

(78 citation statements)

References 55 publications

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“…In the last two decades since the first study by Brittberg et al, its further developments including the third generation autologous chondrocyte implantation using collagen I/III scaffolds has become increasingly popular for treatment of large full thickness cartilage defects. The simplification of the operative procedure had made the third generation ACI more common [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Revision surgery after third generation autologous chondrocyte implantation in the knee

Niethammer

Valentin

Ficklscherer

et al. 2015

International Orthopaedics (SICOT)

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Section: Introductionmentioning

confidence: 99%

Revision surgery after third generation autologous chondrocyte implantation in the knee

Niethammer

Valentin

Ficklscherer

et al. 2015

International Orthopaedics (SICOT)

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“…Currently, there are many treatment options. These include hyaluronic acid injections, to supplement lubrication (Ayhan et al, 2014); subchondral drilling, microfracture and abrasion arthroplasty to stimulate a fibrocartilage repair by bonederived multipotent stromal cells (Pridie, 1959;Steadman et al, 2001;Goyal et al, 2013a;Johnson, 1986;Steinwachs et al, 2008); autologous chondrocyte transplantation or matrix-enhanced chondrocyte implantation (Brittberg et al, 1994;Gillogly et al, 1998;Goyal et al, 2013b); and autologous or allogeneic osteochondral (OC) transplant (Goyal et al, 2014;Sherman et al, 2014). However, none of these treatments result in full restoration of hyaline cartilage function (Orth et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Non-destructive monitoring of viability in an ex vivo organ culture model of osteochondral tissue

Elson

Fox²,

Tipper³

et al. 2015

eCM

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Organ culture is an increasingly important tool in research, with advantages over monolayer cell culture due to the inherent natural environment of tissues. Successful organ cultures must retain cell viability. The aim of this study was to produce viable and non-viable osteochondral organ cultures, to assess the accumulation of soluble markers in the conditioned medium for predicting tissue viability. Porcine femoral osteochondral plugs were cultured for 20 days, with the addition of Triton X-100 on day 6 (to induce necrosis), camptothecin (to induce apoptosis) or no toxic additives. Tissue viability was assessed by the tissue destructive XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide tetrazolium salt) assay method and LIVE/DEAD ® staining of the cartilage at days 0, 6 and 20. Tissue structure was assessed by histological evaluation using haematoxylin & eosin and safranin O. Conditioned medium was assessed every 3-4 days for glucose depletion, and levels of lactate dehydrogenase (LDH), alkaline phosphatase (AP), glycosaminoglycans (GAGs), and matrix metalloproteinase (MMP)-2 and MMP-9. Necrotic cultures immediately showed a reduction in glucose consumption, and an immediate increase in LDH, GAG, MMP-2 and MMP-9 levels. Apoptotic cultures showed a delayed reduction in glucose consumption and delayed increase in LDH, a small rise in MMP-2 and MMP-9, but no significant effect on GAGs released into the conditioned medium. The data showed that tissue viability could be monitored by assessing the conditioned medium for the aforementioned markers, negating the need for tissue destructive assays. Physiologically relevant whole-or part-joint organ culture models, necessary for research and pre-clinical assessment of therapies, could be monitored this way, reducing the need to sacrifice tissues to determine viability, and hence reducing the sample numbers necessary.

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“…These novel techniques are being used since more than a decade with a relatively high success in other musculoskeletal diseases, such as the articular cartilage lesions (D. Goyal, A. Goyal, Keyhani, Lee, & Hui, 2013;Rodriguez-Merchan, 2013;Guillén-Garcia et al, 2014;Demoor et al, 2014). In this work we present in an animal model a new tissue engineering-based technique to treat the ACL tears.…”

Section: Discussionmentioning

confidence: 99%

“…This technique consists on the use of autologous fibroblasts seeded onto porcine type I/III collagen membranes. These membranes have been widely used as a carrier of autologous chondrocytes to treat articular cartilage injuries (Bentley et al, 2013;D. Goyal, A. Goyal, Keyhani, Lee, & Hui, 2013;Guillen et al, 2013;Demoor et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…We have investigated a novel tissue engineering approach based in the use of ACL isolated fibroblasts combined with type I/III collagen membranes as a carrier in the reconstruction of broken ACL. This biomaterial has been widely used as a carrier for other cell types such as chondrocytes for cartilage repair (D. Goyal, A. Goyal, Keyhani, Lee, & Hui, 2013;Rodriguez-Merchan, 2013). We firstly described a method to isolate and culture these cells from ACL biopsies from patients with ACL tears, without losing their biological properties (Guillén et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Use of Autologous Fibroblasts for the Reconstruction of the Anterior Cruciate Ligament Tears

Guillén-García¹,

Rodríguez‐Iñigo²,

Guillén-Vicente³

et al. 2015

IJB

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Surgical reconstruction using an autologous or allogenic allograft is the treatment of choice for anterior cruciate ligament (ACL) tear repair. We have checking the treatment of ACL tears with in vitro cultured autologous fibroblasts on porcine type I/III collagen membranes, in 12 sheep randomly assigned to be treated with collagen membrane without cells or with porcine membrane plus 5 million autologous fibroblasts per cm 2 . The animals underwent a first surgery in which a biopsy of the ACL was taken, and a second one in which the membrane was implanted alone (N=6) or with the fibroblasts (N=6) isolated from the biopsy. After 16 weeks, the animals were sacrificed and samples of healthy and repaired ACL were taken for histological and gene expression of type I collagen (COL1), MMP-13 (matrix metallopeptidase 13), and tenascin-C (TNC) studies. The architecture of normal ACL was not conserved either in the ACL treated with the membrane with or without cells. However, a higher degree of peripheral vascularization was observed in the ACL treated with membrane plus fibroblasts than in those treated with membrane alone. Cellularity and nucleus volume were significantly higher in the center or the periphery of the neo-formed ACL than in the control ACL. No differences between the ACL treated with membrane alone or with membrane plus cells were observed in the relative COL1, MMP-13 and tenascin-C expression. Fibroblasts embedded in porcine type I/III membranes probably enhance vascularization of the graft, so it could be a promising tool for the treatment of ACL tears.

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Evidence-Based Status of Second- and Third-Generation Autologous Chondrocyte Implantation Over First Generation: A Systematic Review of Level I and II Studies

Cited by 98 publications

References 55 publications

Revision surgery after third generation autologous chondrocyte implantation in the knee

Revision surgery after third generation autologous chondrocyte implantation in the knee

Non-destructive monitoring of viability in an ex vivo organ culture model of osteochondral tissue

The Use of Autologous Fibroblasts for the Reconstruction of the Anterior Cruciate Ligament Tears

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