Evidence against involvement of APC mutation in papillary thyroid carcinoma

Ki-ras MUTATIONS IN ADENOMAS: A CHARACTERISTIC OF CANCER-BEARING COLORECTAL MUCOSA

“…17 The region between codons 1286 and 1 5 13 was ampiified in four overlapping sections by PCR and the products were screened for mutations using single-strand conformational polymorphism (SSCP) as previously described. 25 All samples demonstrating band shifts by SSCP analysis were sequenced. Two additional primers were constructed allowing PCR amplification of the complete MCR.…”

Review of the genetics of thyroid tumours: diagnostic and prognostic implications

“…Gardner's syndrome is similarly afflicted with increased incidence of PTC and may be associated with the rare cribriform–morula histological variant 12 . APC mutations have not been found in sporadic classic PTC 13 but have been found in sporadic forms of the cribriform–morula variant 14 . PTC in familial polyposis syndromes often harbours RET /PTC rearrangements (see below) in addition to the APC deletion 15 …”

Germline APC mutation (Gln1317) in a cancer-prone family that does not result in familial adenomatous polyposis

“…Oligonucleotide primer pairs and conditions were as previously described (Curtis et al, 1994a). Each 50 pl reaction mix contained 100 ng DNA, 25 pmoles of each primer, 20 nmoles of each dNTP, 1.25 U thermostable DNA polymerase, and buffer consisting of 10 mM Tris-HCI, pH 8.8, 50 mM KCI, 1.5 mM MgCI,, and 0.1% Triton X-100.…”

“…Mutations here could therefore be detected by PCR amplification of a 43 1 bp sequence using the primers 5'-TCAGAC-GACACAGGAAGCAG-3' and 5'-CTTGGTGGC-ATGGTTTGTC-3'. PCR conditions were as described by Curtis et al (1994a). T h e resulting products were digested with PuuII.…”