Evidence against a Role for Neutrophils in the Clearance of Small Immune Complexes

Hofstaetter, T.; Brammsen, H.

doi:10.1016/s0171-2985(84)80081-9

Cited by 8 publications

(3 citation statements)

References 10 publications

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“…The monoclonal anti-CRl antibody To5 used in the microtiter plate ELISA (30) has been reported to inhibit the capacity to bind erythrocyte-antibody-complement complexes (EAC3b) of RBC (6) and B lymphocytes (21). The present results concerning the experimental system using TT-aTT are in accordance with previous findings as regards the kinetics and saturation of binding complement-opsonized complexes to erythrocyte CRI (7,14,18,19,20). It was established that To5 binds to receptors saturated with IC representing a wide range of size and complementfixing potential (Eig.…”

Section: Discussionsupporting

confidence: 91%

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Erythrocyte CR1 Determination Using Monoclonal Antibody in a Microtiter Plate ELISA; Receptors Are Not Masked by Immune Complexes

Thomsen

Nielsen

Bendixen

1986

Allergy

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The microtiter plate ELISA using monoclonal antibody is a specific, sensitive and quantitative technique for measuring CR1 on human erythrocytes. The present investigations established that receptor occupancy by immune complexes did not affect the measurements. The monoclonal anti-CR1 antibody To5 bound unimpeded to receptors that had reacted with an excess of complement-opsonized tetanus toxoid anti-tetanus toxoid complexes prepared at antigen:antibody ratios between 32:1 and 1:8. The CR1 levels on erythrocytes from 11 patients with systemic lupus erythematosus (SLE) were not increased (P greater than 0.30) after release of CR1-bound immune complexes by incubation with factor I. Neither did the serum from these patients contain blocking anti-CR1 activity (P greater than 0.10). Additionally, the number of antigenic CR1 sites in 10 normals and in the 11 patients with SLE was well correlated with the number of functional receptor sites as assessed by binding of soluble complexes (P less than 0.001). These data establish that the true CR1 levels are determined using the microtiter plate ELISA for quantitation of CR1 in patients with diseases involving immune complexes and/or autoantibodies.

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Section: Discussionsupporting

confidence: 91%

“…Since the CRl has been shown to bind various types of antigen-antibody complexes, indicating that the phenomenon is a general one (25), a well-characterized system (7,20) involving homologous IgG was chosen.…”

Section: Tetanus Toxoid Anti-tetanus Ioxoid Complexes (Tt-att)mentioning

confidence: 99%

Erythrocyte CR1 Determination Using Monoclonal Antibody in a Microtiter Plate ELISA; Receptors Are Not Masked by Immune Complexes

Thomsen

Nielsen

Bendixen

1986

Allergy

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“…Precipitated Te/aTe diluted 1:10 in VBS corresponded to strongly immune complexed posi-tive sera by quantitation in the complement consumption step of the PEG-CC assay (3), while Te/aTe diluted 1:100 were negative in the test. As expected these soluble immune complexes (Te/aTe "S") were not detectable by analysis in the complement consumption step of the PEG-CC assay (3,13).…”

Section: Teuifius Toxoid Anti-tetanus Toxoid Complexessupporting

confidence: 71%

Inhibition of Natural Killer Cell Activity by Antigen‐Antibody Complexes

Pedersen

Thomsen

Nielsen

1986

Allergy

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The natural killer (NK) cell activity of human peripheral blood mononuclear cells was found to be inhibited by precipitated tetanus toxoid anti-tetanus toxoid complexes (Te/aTe) as well as soluble Te/aTe. Preincubation of the immune complexes with protein A decreased the inhibition of NK cell activity. When mononuclear cells were preincubated with interferon (IF) or interleukin 2 (Il-2) before incubation with Te/aTe, the immune complex-induced inhibition was decreased, while IF or Il-2 added after incubation with the immune complexes had no effect. Using NK cell-enriched suspensions in a single cell agarose assay, the immune complexes were shown to inhibit NK cell activity by inhibiting the formation of effector/target cell conjugates.

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The Fc valency of an immune complex is the decisive factor for binding to low‐affinity Feγ receptors

et al. 1988

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Tetanus toxoid (TT) was complexed with two human monoclonal antibodies. The antibodies recognized different, nonrepeating epitopes. The complexes formed were characterized by gel filtration and isokinetic sucrose density gradient centrifugation. It was found that in antigenic excess the separate antibodies formed a complex of one antibody molecule and two TT molecules [IgG1-(TT)2 and IgG3-(TT)2]. In cases where equal amounts of TT and both antibodies were mixed, a dimeric complex [IgG1-(TT)2-IgG3] was formed. The binding of these immune complexes to human neutrophils and eosinophils was studied. Whereas the immune complexes containing one antibody did not bind to either cell type, the two-antibody complex bound to both. This indicates that not the sterical change in the Fc part of an antibody molecule after binding an antigen, but the Fc valency of an immune complex is the decisive factor in Fc receptor interaction with neutrophilic and eosinophilic granulocytes.

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Evidence against a Role for Neutrophils in the Clearance of Small Immune Complexes

Cited by 8 publications

References 10 publications

Erythrocyte CR1 Determination Using Monoclonal Antibody in a Microtiter Plate ELISA; Receptors Are Not Masked by Immune Complexes

Erythrocyte CR1 Determination Using Monoclonal Antibody in a Microtiter Plate ELISA; Receptors Are Not Masked by Immune Complexes

Inhibition of Natural Killer Cell Activity by Antigen‐Antibody Complexes

The Fc valency of an immune complex is the decisive factor for binding to low‐affinity Feγ receptors

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