Eversporting as a Function of the Y-Chromosome in Drosophila Melanogaster

Gowen, John W.

doi:10.1073/pnas.19.1.122

Cited by 37 publications

(10 citation statements)

References 5 publications

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“…PEV resulting from genomic rearrangements has been shown to be suppressed by additional heterochromatin (Gowen and Gay 1933;for review, see Spofford 1976). To test whether additional heterochromatin suppresses the PEV of the inserts, males of a given transgenic line showing PEV of hsp70--white were crossed to white m u t a n t females with an attached-X chromosome (X X^).…”

Section: A Screen For Pev Of White Expression Results In Recovery Of mentioning

confidence: 99%

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Position effect variegation in Drosophila is associated with an altered chromatin structure.

Wallrath¹,

Elgin²

1995

Genes Dev.

464

400

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A euchromatic gene placed in the vicinity of heterochromatin by a chromosomal rearrangement generally exhibits position effect variegation (PEV), a clonally inherited pattern showing gene expression in some somatic cells but not in others. The mechanism responsible for this loss of gene expression is investigated here using fly lines carrying a P element containing the Drosophila melanogaster white and hsp26 genes. Following mobilization of the P element, a screen for variegation of white expression recovered inserts at pericentric, telomeric, and fourth chromosome regions. Previously identified suppressors of PEV suppressed white variegation of pericentric and fourth chromosome inserts but not telomeric inserts on the second and third chromosomes. This implies a difference in the mechanism for gene repression at telomeres. Heat shock-induced hsp26 expression was reduced from pericentric and fourth chromosome inserts but not from telomeric inserts. Chromatin structure analysis revealed that the variegating inserts showed a reduction in accessibility to restriction enzyme digestion in the hsp26 regulatory region in isolated nuclei. Micrococcal nuclease digests showed that pericentric inserts were packaged in a more regular nucleosome array than that observed for euchromatic inserts. These data suggest that altered chromatin packaging plays a role in PEV.

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Section: A Screen For Pev Of White Expression Results In Recovery Of mentioning

confidence: 99%

“…In support of the mass-action model, suppression of PEV also occurs upon addition of heterochromatic material. Heterochromatic sequences such as those that make up most of the Y chromosome appear to titrate heterochromatic pro-teins, resulting in suppression of PEV (Gowen and Gay 1933).…”

Section: Possible Molecular Mechanisms Responsible For Pevmentioning

confidence: 99%

Position effect variegation in Drosophila is associated with an altered chromatin structure.

Wallrath¹,

Elgin²

1995

Genes Dev.

464

400

View full text Add to dashboard Cite

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“…Such protection operates in, for example, Cyclops, in which there is loss of heterochromatic segments during embryogenesis (Beermann, 1966). Addition of heterochromatin, such as an extra Y chromosome, usually reduces the amount of mutant tissue in a case of P-EV (Gowen & Gay, 19336) and this appears to favour the suppression rather than the mutation hypothesis. But even the former hypothesis faces difficulties; polarization and the cis-trans effect indicate a highly localized, intrachromosomal effect while, relatively speaking, the extra heterochromatin acts at a distance.…”

Section: Discussionmentioning

confidence: 99%

Mitotic non-conformity inAspergillus nidulans: the production of hypodiploid and hypohaploid nuclei

Roper

Nga

1969

Genet. Res.

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Strains of Aspergillus nidulans with a chromosome segment additional to the normal complement are vegetatively unstable. Previous work suggested that the deletions occurring at mitosis were confined to the unbalanced segments. It has been shown now that deletions, while probably always involving a duplicate segment, may extend beyond it to produce hypohaploids and hypodiploids, respectively, from unbalanced haploid and unbalanced diploid parents.Hypoploids have been proposed tentatively as an explanation for some cases of phenotypic variegation; on this basis it is possible to account for some of the diverse phenomena shown by, for example, position-effect variegation.

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“…The description of PEV is based on the mosaic cell to cell variation of white (w) gene expression [55]. PEV is characteristic to specific chromosomal rearrangements of the w locus, such as w m4 [56]. In flies containing the w m4 rearrangement, the normally euchromatic white gene is displaced into the vicinity of a heterochromatic region.…”

Section: Evolutionarily Conserved Domains In Pcg Proteinsmentioning

confidence: 99%

From Flies to Mice: The Emerging Role of Non-Canonical PRC1 Members in Mammalian Development

2018

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Originally two types of Polycomb Repressive Complexes (PRCs) were described, canonical PRC1 (cPRC1) and PRC2. Recently, a versatile set of complexes were identified and brought up several dilemmas in PRC mediated repression. These new class of complexes were named as non-canonical PRC1s (ncPRC1s). Both cPRC1s and ncPRC1s contain Ring finger protein (RING1, RNF2) and Polycomb group ring finger catalytic (PCGF) core, but in ncPRCs, RING and YY1 binding protein (RYBP), or YY1 associated factor 2 (YAF2), replaces the Chromobox (CBX) and Polyhomeotic (PHC) subunits found in cPRC1s. Additionally, ncPRC1 subunits can associate with versatile accessory proteins, which determine their functional specificity. Homozygous null mutations of the ncPRC members in mice are often lethal or cause infertility, which underlines their essential functions in mammalian development. In this review, we summarize the mouse knockout phenotypes of subunits of the six major ncPRCs. We highlight several aspects of their discovery from fly to mice and emerging role in target recognition, embryogenesis and cell-fate decision making. We gathered data from stem cell mediated in vitro differentiation assays and genetically engineered mouse models. Accumulating evidence suggests that ncPRC1s play profound role in mammalian embryogenesis by regulating gene expression during lineage specification of pluripotent stem cells.

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Eversporting as a Function of the Y-Chromosome in Drosophila Melanogaster

Cited by 37 publications

References 5 publications

Position effect variegation in Drosophila is associated with an altered chromatin structure.

Position effect variegation in Drosophila is associated with an altered chromatin structure.

Mitotic non-conformity inAspergillus nidulans: the production of hypodiploid and hypohaploid nuclei

From Flies to Mice: The Emerging Role of Non-Canonical PRC1 Members in Mammalian Development

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