Evenly tritium labeled peptides in study of peptide in vivo and in vitro biodegradation

Zolotarev, Yu. A.; Dadayan, A. K.; Долотов, О. В.; Kozik, V. S.; Кост, Н. В.; Sokolov, O. Yu.; Dorokhova, E. M.; Meshavkin, V. K.; Иноземцева, Л. С.; Gabaeva, M. V.; Andreeva, L. A.; Alfeeva, L. Yu.; Pavlov, Tengis S.; Badmaeva, K E; Бадмаева, С Э; Бакаева, З. В.; Kopylova, G. N.; Samonina, G. E.; Vaskovsky, B. V.; Гривенников, И. А.; Zozulya, A. A.; Myasoedov, N. F.

doi:10.1134/s1068162006020099

Cited by 14 publications

(14 citation statements)

References 16 publications

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“…However, incomplete or low degree of deuteration significantly complicates studies of various kinetic or biotransformation processes. In this context, deuterated peptides containing the isotope label in each amino acid residue provide new opportunities to obtain accurate and correct results …”

mentioning

confidence: 99%

Use of deuterium labeling by high‐temperature solid‐state hydrogen‐exchange reaction for mass spectrometric analysis of bradykinin biotransformation

Kopylov

Myasoedov

Dadayan

et al. 2016

Rapid Comm Mass Spectrometry

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mentioning

confidence: 99%

Use of deuterium labeling by high‐temperature solid‐state hydrogen‐exchange reaction for mass spectrometric analysis of bradykinin biotransformation

Kopylov

Myasoedov

Dadayan

et al. 2016

Rapid Comm Mass Spectrometry

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“…The sites reported to be most highly labeled in aliphatic amino acids are Ca, methyl groups and carbons adjacent to O or N; benzyl methylenes of aromatic amino acids, and aryl sites activated for electrophilic substitution. In five 3-7mer peptides the inter-residue tritium distribution (totalling 50-110 Ci/mmol) was not too dissimilar from that expected for individual amino acids 259 (1:10 coating of peptide on Al 2 O 3 mechanically mixed with 10 parts 5% Rh/Al 2 O 3 , 140-190 C, 10-60 min). In five 3-7mer peptides the inter-residue tritium distribution (totalling 50-110 Ci/mmol) was not too dissimilar from that expected for individual amino acids 259 (1:10 coating of peptide on Al 2 O 3 mechanically mixed with 10 parts 5% Rh/Al 2 O 3 , 140-190 C, 10-60 min).…”

Section: High-temperature Solid-state Catalytic Isotope Exchangementioning

confidence: 99%

Preparation of Tritium‐Labeled Compounds by Isotope Exchange Reactions

Voges¹,

Heys

Moenius

2009

Preparation of Compounds Labeled With Tritium and Carbon‐14

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“…Primary biodegradation of Selank occurs under the influence of the dipeptidyl carboxypeptidases, which are responsible for the formation of the pentapeptide Thr-Lys-Pro-Arg-Pro and the longest-lasting tripeptide Thr-Lys-Pro and dipeptides Arg-Pro and Gly-Pro; these peptides seem to have largely Selank-like effects. The subsequent decay continues until the degradation to individual amino acids, the fate of which in the body is no different to that of the naturally occurring amino acids [183].…”

Section: Pharmacokineticsmentioning

confidence: 99%

“…The content of Selank in the brain after intranasal administration was comparable to that observed in the blood, remained quite stable, and declined more slowly than it did in the blood and in the organs of the abdominal cavity. In vitro experiments revealed that the half-life of Selank is ~2 min [183].…”

Section: Pharmacokineticsmentioning

confidence: 99%

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

Kolomin¹,

Шадрина²,

Slominsky³

et al. 2013

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Natural regulatory peptides are biologically active compounds that are produced by various cells and provide a link among the main regulatory systems of the body. The field of research into the biologic activity of endogenous regulatory peptides is extremely vast. These peptides affect the cardiovascular, immune, reproductive, endocrine, digestive, and other systems, alter energy metabolism, and are especially effective in the regulation of the central nervous system. Despite of the wide range of preventive and therapeutic effects of natural regulatory peptides and proteins, their application in clinical practice is difficult. This is primarily because of their extreme instability, as they are rapidly degraded by proteases of the gastrointestinal tract, blood, cerebrospinal fluid, and other biologic media. Compounds with higher stability (i.e., a considerably longer half-life compared with that of natural molecules) and the ability to provide a directional effect on the various body systems were obtained from modifications of endogenous regulatory peptides. Synthetic analogs of regulatory peptides, as a rule, contain only natural amino acids in their composition, and their biodegradation does not lead to the formation of toxic products; thus, they have fewer side effects. This review focuses on the consideration of two synthetic regulatory peptides, Semax and Selank, which were the bases for the creation of new drugs that are used effectively in the treatment of various diseases of the nervous system. The synthetic analog of an adrenocorticotropic hormone 4-10 fragment (ACTH 4-10) Semax is a powerful neuroprotective agent that is particularly effective as a therapy for stroke. Selank was synthesized on the basis of the natural immunomodulator tuftsin. Selank is a powerful anxiolytic that is used as a therapy for generalized anxiety disorder and neurasthenia without sedative and muscle-relaxant effects. This review presents the results of research aimed at studying the influence of these peptides on the transcriptome of brain cells. The problems of drugs developed based on the clinical activities of Semax and Selank are discussed separately.

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Evenly tritium labeled peptides in study of peptide in vivo and in vitro biodegradation

Cited by 14 publications

References 16 publications

Use of deuterium labeling by high‐temperature solid‐state hydrogen‐exchange reaction for mass spectrometric analysis of bradykinin biotransformation

Use of deuterium labeling by high‐temperature solid‐state hydrogen‐exchange reaction for mass spectrometric analysis of bradykinin biotransformation

Preparation of Tritium‐Labeled Compounds by Isotope Exchange Reactions

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

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