Evenly Distributed Thin-Film Ag Coating on Stainless Plate by Tricomponent Ag/Silicate/PU with Antimicrobial and Biocompatible Properties

Abstract

A tricomponent nanohybrid dispersion in water comprising silver nanoparticles (AgNP), nanometer-thick silicate platelets (NSP), and water-based polyurethane (PU) was developed for surface coating on orthopedic metal plates. The previously developed AgNP-on-NSP nanohybrid was homogeneously blended into a selected waterborne PU dispersion at varied weight ratios from 1/0.1 to 1/10 (w/w). PU was used to adhere the Ag nanohybrid to the metal surface. The resultant dispersions were analyzed and found to contain AgNP 2-18 nm in diameter and characterized by using UV absorption and TEM micrograph. The subsequent coating of AgNP/NSP-PU dispersion generated a film of 1.5 μm thickness on the metal plate surface, further characterized by an energy dispersive spectroscope (EDS) to show the homogeneous distribution of Ag, Si, and C elements on the metal plates. The surface antimicrobial efficacy was proven for the coating composition of AgNP/NSP to PU ranging from 1/1 to 1/5 by weight ratio but irrelevant to the thickness of the coated materials. The metal plate coated with the high Ag content at 1/1 (w/w) ratio was shown to have very low cytotoxicity toward the contacted mammal fibroblasts. Overall, the optimized tricomponent Ag/silicate/PU in water dispersion from 1/2 to 1/3 (w/w) could generate a stable film on a metal surface exhibiting both antimicrobial and biocompatible properties. The facile coating technique of the AgNP/NSP in waterborne PU is proven to be viable for fabricating infection- and cytotoxicity-free medical devices.

3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity

“…The NCTC clone 929 (L-929) cell line was purchased from American Type Culture Collection (ATCC). All of the procedures were performed according to our previous research [ 25 ]. Cells were seeded in 24-well tissue culture plates at a density of 2 ×10 4 cells per well.…”

Composition of nanoclay supported silver nanoparticles in furtherance of mitigating cytotoxicity and genotoxicity

“…Nanomaterials with different physical properties and compositions [1][2][3] may give rise to new applications in biomedicines [4], drug-delivery agents [5,6], or biosensors [7][8][9]. Silver nanoparticles (Ag-NP) have been identified as effective antimicrobial agents and have been widely utilized as bactericides [10,11] in many medical fields such as wound dressings, catheters, implants coating, and bone cements [12][13][14]. The antimicrobial mechanisms of Ag-NP involve the process of adhesion onto the cell surface (impairment of membrane structure and permeability), penetration of the cell and dysfunction of intracellular structures (mitochondria and ribosomes), induction of reactive oxygen species (ROS) and denaturation of biomolecules (protein, lipid and DNA), and modulation of signal transduction pathways [15][16][17][18][19][20].…”

Microstructure and antibacterial performance of functionalized polyurethane based on polysiloxane tethered cationic biocides

“…The effective approach to reduce this bacteria-associated contamination is to endow the materials with active groups to kill or release the attached bacteria. [18][19][20] In recent years, various antibacterial polyurethanes have been designed by incorporation of a low molecular biocides, including organic antibiotics, [21][22][23][24] silver [25][26][27] and triclosan. 28,29 However, the duration and efficiency of antibacterial action were limited by loading and release kinetics of the biocides contained in materials.…”