Ammonium acetate (NH 4 Ac) is a widely used solvent additive in native electrospray ionization (ESI) mass spectrometry. NH 4 Ac can undergo proton transfer to form ammonia and acetic acid (NH 4 + + Ac − → NH 3 + HAc). The volatility of these products ensures that electrosprayed ions are free of undesired adducts. NH 4 Ac dissolution in water yields pH 7, providing "physiological" conditions. However, NH 4 Ac is not a buffer at pH 7 because NH 4 + and Ac − are not a conjugate acid/ base pair (Konermann, L.